Türkcan Kayhan, Ceren

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Profile URL
https://hdl.handle.net/20.500.14365/7279
Name Variants
Kayhan, Ceren Turkcan
Job Title
Email Address
ceren.turkcan@ieu.edu.tr
Main Affiliation
15.06. Medical Laboratory Techniques
Status
Former Staff
Website
ORCID ID
0000-0002-3354-5707
Scopus Author ID
55542492900
Turkish CoHE Profile ID
68F5824C197B2E03
Google Scholar ID
vjRBXnUAAAAJ
WoS Researcher ID
AAY-2517-2020

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Journal of Bıomaterıals Scıence-Polymer Edıtıon1
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    Article
    Citation - WoS: 6
    Citation - Scopus: 6
    Dna Isolation by Galactoacrylate-Based Nano-Poly(hema Nanopolymers
    (Taylor & Francis Ltd, 2017) Kayhan, Ceren Turkcan; Ural, Fulden Zeynep; Koruyucu, Meryem; Salman, Yesim Gul; Uygun, Murat; Uygun, Deniz Aktas; Akgol, Sinan
    Isolation of DNA is one of the important processes for biotechnological applications such as investigation of DNA structures and functions, recombinant DNA preparations, identification of genetic factors and diagnosis and treatment of genetic disorders. The aim of this study was to synthesis and characterizes the galactoacrylate based nanopolymers with high surface area and to investigate the usability of these synthesized nanopolymers for DNA isolation studies. Nanopolymers were synthesized by the surfactant free emulsion polymerization technique by using the monomers of 2-hydroxyl ethylmethacrylate and 6-O-(2'-hydroxy-3'-acryloyloxypropyl)-1,2:3,4-di-O-isopropylidene-alpha-D-galactopyranose. Galactoacrylate origin of these newly synthesized nanopolymers increased the interaction between DNA and nanopolymers. Prepared nanopolymers were characterized by SEM, FT-IR and ZETA sizer analysis. Synthesized nanopolymers were spherical, and their average particle size was about 246.8nm. Adsorption of DNA onto galactoacrylate based nanopolymers was investigated by using different pHs, temperatures, ionic strength, DNA concentrations and desorption studies and maximum DNA adsorption was found to be as 567.12 mg/g polymer at 25 degrees C, in pH 5.0 acetate buffer. Reusability was investigated for 5 successive reuse and DNA adsorption capacity decreased only about 10% at the end of the 5th reuse.