Kılıç, Yalın
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Kilic, Yalin
Job Title
Email Address
kilic.yalin@ieu.edu.tr
Main Affiliation
05.02. Biomedical Engineering
Status
Current Staff
Website
ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Sustainable Development Goals
Documents
9
Citations
144
h-index
6
Documents
0
Citations
0
Scholarly Output
4
Articles
4
Views / Downloads
4/0
Supervised MSc Theses
0
Supervised PhD Theses
0
WoS Citation Count
35
Scopus Citation Count
36
WoS h-index
4
Scopus h-index
4
Patents
0
Projects
0
WoS Citations per Publication
8.75
Scopus Citations per Publication
9.00
Open Access Source
2
Supervised Theses
0
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4 results
Scholarly Output Search Results
Now showing 1 - 4 of 4
Article Citation - WoS: 4Citation - Scopus: 4Low-Cost High-Resolution Potentiostat for Electrochemical Detection of Nucleic Acids and Biomolecular Interactions(Mdpi, 2022) Demirhan, Alper; Eksin, Ece; Kilic, Yalin; Erdem, ArzumA handheld USB-powered instrument developed for the electrochemical detection of nucleic acids and biomolecular interactions is presented. The proposed instrument is capable of scanning +/- 2.25 V while measuring currents up to +/- 10 mA, with a minimum current resolution of 6.87 pA. Therefore, it is suitable for nucleic acid sensors, which have high background currents. A low-cost microcontroller with an on-chip 16-bit analog-to-digital converter, 12-bit digital-to-analog converter, and a built-in USB controller were used to miniaturize the system. The offset voltages and gain errors of the analog peripherals were calibrated to obtain a superior performance. Thus, a similar performance to those of the market-leader potentiostats was achieved, but at a fraction of their cost and size. The performance of the application of this proposed architecture was tested successfully and was found to be similar to a leading commercial device through a clinical application in the aspects of the detection of nucleic acids, such as calf thymus ssDNA and dsDNA, and their interactions with a protein (BSA) by using single-use graphite electrodes in combination with the differential pulse voltammetry technique.Article Citation - WoS: 5Citation - Scopus: 6Brief Fine Polishing of Thin-Film Gold Electrode Sensors Leads To Better Reproducibility Than Electrochemical Pretreatment(Esg, 2020) Kilic, Yalin; Manickham, Pandiaraj; Bhansali, ShekharSurface roughness of the electrode and its activation is critical for improved reproducibility of biosensors. Electrochemical cycling has traditionally been the preferred approach for electrochemical activation with only a few reports of mechanical polishing as the surface activation technique. This study compares the efficiency of mechanical polishing and electrochemical activation of thin film gold electrode surfaces for electrochemical measurements. The effects of the approach on both the electrochemical activity and surface variations were studied. Our findings suggest that brief polishing with 50 nm alumina nanoparticles resulted in improved electrochemical activity and better electrochemical area and roughness control when compared to the electrochemically activated counterparts.Article Citation - WoS: 5Citation - Scopus: 4A Novel Smartphone-Based Nanozyme-Enhanced Electrochemical Immunosensor for Ultrasensitive Direct Detection of Staphylococcus Aureus in Milk and Blood Serum(Elsevier B.V., 2025) Savas, Sumeyra; Kilic, Yalin; Gharibzahedi, Seyed Mohammad Taghi; Altintas, ZeynepA graphene quantum dot (GQD)-based nanozyme-enhanced electrochemical sensor was developed for the ultra-sensitive and rapid Staphylococcus aureus detection in PBS, undiluted milk, and blood serum. The S. aureus concentration for immunosensor calibration was electrochemically determined to be 1.24x 10(9) CFU mL(-1) (R-2 = 0.99), utilizing an innovative smartphone-based electrochemical device. Transmission electron (TEM) and atomic force (AFM) microscopy analyses of GQDs revealed uniform nanoparticles (8-10 nm) with surface heights of 2 nm and 21 nm at different scan areas. Fluorescence and Fourier transform infrared spectroscopy spectra showed an emission at 530 nm with an excitation wavelength of 485 nm, along with the presence of hydroxyl, carboxyl, and aromatic groups on the GQD surfaces. The maximum current peak for GQDs was obtained by optimizing electrochemical properties through amperometry at +0.9 V. The optimal signal intensity- and limit of detection (LOD)-based GQD concentrations were 0.0125, 0.5, and 1 mg mL(-1) for PBS, undiluted milk, and blood serum, respectively. The optimal antibody concentration for S. aureus detection was 25 mu g mL(-1), resulting in a 91 % amperometric suppression and a 94 % fluorescence quenching. The developed biosensor effectively detected S. aureus, with calculated LODs of 1, 4, and 344 CFU mL(-1) in PBS, undiluted milk, and blood serum, respectively. TEM, AFM, and contact angle analyses confirmed S. aureus binding to the GQD-antibody bioconjugate, shifting surface height to 35 nm and contact angle from similar to 30 degrees to similar to 65 degrees. The immunosensor showed high specificity for S. aureus with minimal cross-reactivity to Listeria monocytogenes, Staphylococcus epidermidis, Enterococcus faecium, and Streptococcus pneumoniae.Article Citation - WoS: 21Citation - Scopus: 22Quantitative Determination of H2o2 for Detection of Alanine Aminotransferase Using Thin Film Electrodes(Academic Press Inc Elsevier Science, 2020) Saygili, Ecem; Orakci, Beyza; Koprulu, Melisa; Demirhan, Alper; Ilhan-Ayisigi, Esra; Kilic, Yalin; Yesil-Celiktas, OzlemThe abnormal concentrations or absence of biomolecules (e.g., proteins) in blood can further be used in diagnosis of a particular pathology at an early stage. Current studies are intensely focusing on the analysis of interaction and detection of biomolecules via point-of-care systems (POCs), allowing miniaturized and parallelized reactions, simultaneously. Recent developments have shown that the collaboration of electrochemical sensing techniques and POCs to overcome challenging problems in health-care settings provides new approaches in diagnosis and treatment of diseases. The aim of this study was to adapt the alanine aminotransferase (ALT) enzyme to the platinum (Pt) thin film electrode system and quantitatively determine the enzyme levels via enzymatically generated H2O2 with differential pulse voltammetry (DPV). A simple potentiostat architecture with expanded sweep range utilizing dual LMP91000 devices was developed and adapted to the needs of the biosensor. In order to calibrate the system, known concentrations of H2O2 were also tested. Moreover, signals associated with the other electroactive species coming from the ALT reaction were eliminated. Resulted potential range has been achieved between +500 mV and + 900 mV and the linear range was found to be 0.05 M-0.5 M for H2O2, whereas 5 UL-1 to 120 UL-1 for ALT enzyme.
