Kurul, F.Avci, M.B.Yaman, S.Topkaya, S.N.Cetin, A.E.2024-08-252024-08-252024978-151067334-20277-786Xhttps://doi.org/10.1117/12.3025745https://hdl.handle.net/20.500.14365/5478The Society of Photo-Optical Instrumentation Engineers (SPIE)Biophotonics in Point-of-Care III 2024 -- 10 April 2024 through 12 April 2024 -- Strasbourg -- 200984Compact and portable biosensing technologies play an important role in replacing traditional counterparts that require costly and heavy equipment, as well as complex infrastructure. The integration of these easy-to-use and cheap devices allows for the conducting of biosensing analyses in resource-limited settings. The study produced a portable optofluidic platform that is lightweight (260 g) and compact (16 cm×10 cm×11 cm). It combines subwavelength nanohole arrays, microfluidics technology, and on-chip computational imaging. It records plasmonic diffraction field images with a CMOS imager and an LED light, allowing for a large field of view for refractive index measurement. This LED source generates diffraction patterns on the imager. The microfluidic pump confirms accurate analyte delivery, allowing real-time analysis of diffraction field images to reveal time-dependent binding kinetics of biomolecules. It identifies biomolecular interactions without labelling, allowing for the detection and quantification of biomolecules. Our platform has an outstanding limit-of-detection (LOD) of 5ng/mL for label-free detection of protein IgG. We effectively determined the association and dissociation constants for protein A/G and IgG binding using real-time diffraction field images. The optofluidic biosensor platform is ideal for surface plasmon resonance (SPR) in field applications. It can monitor interactions in real-time, making it useful for studying the way various biological and chemical compounds bind in many areas. © 2024 SPIE.eninfo:eu-repo/semantics/closedAccessComputational imagingLabel-free biosensingMicrofluidicsPlasmonicsComputational ImagingDiffractionDissociationLight emitting diodesMicrofluidicsPlasmonicsProteinsRefractive indexSurface plasmon resonanceBiosensingComputational imagingDiffraction fieldsDynamic bindingField imagesIn-fieldLabel-free biosensingOptofluidic devicesPlasmonicsReal- timeBiomoleculesConference Object10.1117/12.30257452-s2.0-85199071516