Büyüktuna, S.A.Yerlitaş, S.İ.Zararsız, G.E.Doğan, K.Kablan, D.Bağcı, G.Özer, S.2024-06-012024-06-0120240146-66151096-9071https://doi.org/10.1002/jmv.29672https://hdl.handle.net/20.500.14365/5357This study investigated the intricate interplay between Crimean-Congo hemorrhagic fever virus (CCHFV) infection and alterations in amino acid metabolism. Our primary aim is to elucidate the impact of Crimean-Congo hemorrhagic fever (CCHF) on specific amino acid concentrations and identify potential metabolic markers associated with viral infection. One hundred ninety individuals participated in this study, comprising 115 CCHF patients, 30 CCHF negative patients, and 45 healthy controls. Liquid chromatography-tandem mass spectrometry techniques were employed to quantify amino acid concentrations. The amino acid metabolic profiles in CCHF patients exhibit substantial distinctions from those in the control group. Patients highlight distinct metabolic reprogramming, notably characterized by arginine, histidine, taurine, glutamic acid, and glutamine metabolism shifts. These changes have been associated with the underlying molecular mechanisms of the disease. Exploring novel therapeutic and diagnostic strategies addressing specific amino acids may offer potential means to mitigate the severity of the disease. © 2024 Wiley Periodicals LLC.eninfo:eu-repo/semantics/closedAccessamino acidCrimean-Congo hemorrhagic feverLC-MS/MSseverityalanine aminotransferasealkaline phosphataseamino acidamylasearginineaspartate aminotransferasecreatine kinasecreatinineethanolamineferritingamma glutamyltransferaseglutamic acidglutaminehistidineimmunoglobulin Minterleukin 6lactate dehydrogenasemethionineornithinephenylalanineribavirinsteroidtaurinetyrosineamino acidbiological markeractivated partial thromboplastin timeadultamino acid metabolismarea under the curveArticlebiochemical analysisblood clottingcontrolled studyCrimean Congo hemorrhagic feverdiagnostic test accuracy studydisease exacerbationdisease severityelectrochemiluminescenceelectrosprayenzyme linked immunosorbent assayfasting blood glucose levelfemalehematocrithemoglobin blood levelhepatomegalyhumanintensive care unitleukocyte countliquid chromatography-mass spectrometryliver dysfunctionliver function testlymphocyte countmajor clinical studymalemetabolic reprogrammingmultiple reaction monitoringneutrophil countplatelet countreal time polymerase chain reactionreceiver operating characteristicscoring systemseverity grading scorevirus infectionagedliquid chromatographymetabolismmiddle agedtandem mass spectrometryAdultAgedAmino AcidsBiomarkersChromatography, LiquidDisease ProgressionFemaleHumansMaleMiddle AgedTandem Mass SpectrometryArticle10.1002/jmv.296722-s2.0-85193380919