Sarigul, FigenDoluca, OsmanAkhan, SilaSayan, Murat2023-06-162023-06-1620201746-07941746-0808https://doi.org/10.2217/fvl-2020-0169https://hdl.handle.net/20.500.14365/2390Aim:In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.Materials & methods:Samples were tested by Bio-Speddy(R)(RdRpgene) and Diagnovital(R)(RdRp + Egenes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.Results:Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.Conclusion:We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.eninfo:eu-repo/semantics/openAccesscoronavirusCOVID-19RT-PCRSARS-CoV-2OutbreakArticle10.2217/fvl-2020-01692-s2.0-85091075065