Aydeniz, BurhanMetin, Safa CanTurkan, MehmetUnay, DevrimKarayannis, TheofanisArgunsah, Ali Ozgur2023-06-162023-06-162022978-1-6654-5432-2https://doi.org/10.1109/TIPTEKNO56568.2022.9960191https://hdl.handle.net/20.500.14365/1997Medical Technologies Congress (TIPTEKNO) -- OCT 31-NOV 02, 2022 -- Antalya, TURKEYTwo-photon Laser Scanning Microscopy (2P-LSM) is a technique used to image the living tissue with relatively high spatio-temporal resolution. However, the time-series images are often corrupted with Poisson-Gaussian noise and deteriorated with motion artifacts. This paper deals with the problem of enhancing 2P-LSM images to reconstruct high quality and high spatial-resolution outputs using the observed time-series stack of low-resolution images. The proposed technique consists of several components including noise filtering, image registration, cell detection and focus measurement, and clustering for a joint denoising and super-resolution. Extensive experiments demonstrate that the proposed method results in gratifying output images containing apparent and clear cell forms at different focus levels.eninfo:eu-repo/semantics/closedAccessTwo-photon laser scanning microscopydenoisingsuper-resolutionclusteringMicroscopyFocusShapeConference Object10.1109/TIPTEKNO56568.2022.99601912-s2.0-85144081696