Demir, Esra FeyziogluAtay, Nevra OzturkKoruyucu, MeryemKok, GokhanSalman, YesimAkgol, Sinan2023-06-162023-06-1620180149-63951520-5754https://doi.org/10.1080/01496395.2018.1452943https://hdl.handle.net/20.500.14365/1592The aim of this work is to synthesize the original, new polymeric nanoparticles for concanavalin A (Con A) purification. Nanoparticles were synthesized by surfactant free emulsion polymerization. In the polymerization prosedure, 1-O-(2'-hydroxy-3'-acryloyloxypropyl)-2,3:5,6-di-O-isopropylidene-alpha-D-mannofuranose (Man-OPA) was used as co-monomer and 2-hydroxyethylmethacrylate (HEMA) was used as a monomer. Man-OPA was characterized by Fourier Transform Infrared Spectroscopy (FTIR), nuclear magnetic resonance and elemental analysis techniques. Poly(HEMA-Man-OPA) nanoparticles were characterized by scanning electron microscopy, FTIR and Zeta Sizer. In adsorption-desorption experiments, maximum Con A adsorption capacity of poly(HEMA-Man-OPA) nanoparticles was found 630.6 mg/g nanoparticle (pH 7.5, 1.0 mg/mL). Adsorption-desorption experiments were repeated in four times. According to results, these nanoparticles could be used several times without significant decrease in Con A adsorption capacity.eninfo:eu-repo/semantics/closedAccessLectinconcanavalin Ananoparticlecarbohydrate acrylateadsorptionConcanavalin-AAffinity-ChromatographyAntibody PurificationReversible UseAdsorptionNanospheresMoleculesSurfaceLayerArticle10.1080/01496395.2018.14529432-s2.0-85049949214