Bulgurcu, Alihan

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Profile URL
https://hdl.handle.net/20.500.14365/6784
Name Variants
BULGURCU, ALIHAN
Job Title
Email Address
alihan.bulgurcu@ieu.edu.tr
Main Affiliation
15.06. Medical Laboratory Techniques
Status
Current Staff
Website
ORCID ID
0000-0001-7422-4494
Scopus Author ID
57225054896
Turkish CoHE Profile ID
5B7D6D1268F0AFDF
Google Scholar ID
WUvDZqAAAAAJ
WoS Researcher ID
GST-1402-2022
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Alihan_Bulgurcu.png (76.8 KB)

Sustainable Development Goals

5

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9

INDUSTRY, INNOVATION AND INFRASTRUCTURE
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13

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8

DECENT WORK AND ECONOMIC GROWTH
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14

LIFE BELOW WATER
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17

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1

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2

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4

QUALITY EDUCATION
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11

SUSTAINABLE CITIES AND COMMUNITIES
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16

PEACE, JUSTICE AND STRONG INSTITUTIONS
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GOOD HEALTH AND WELL-BEING
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6

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15

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4

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Scholarly Output

4

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3

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WoS Citation Count

9

Scopus Citation Count

13

WoS h-index

2

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2

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WoS Citations per Publication

2.25

Scopus Citations per Publication

3.25

Open Access Source

3

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BMC Microbiology1
FEBS Open Bio1
Mıkrobıyolojı Bultenı1
Turkısh Journal of Bıochemıstry-Turk Bıyokımya Dergısı1
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Now showing 1 - 4 of 4
  • Thumbnail Image
    Article
    Citation - WoS: 2
    Citation - Scopus: 2
    Analysis of Nucleotide Changes in Rt-Pcr Primer/Probe Binding Regions in Sars-Cov Isolates Reported From Turkey
    (Ankara Microbiology Soc, 2021) Demir, Ayse Banu; Bulgurcu, Alihan; Appak, Ozgur; Sayiner, Ayca Arzu
    The SARS-CoV-2 virus, which caused the COVID-19 epidemic, caused more than 55 million cases and nearly 1.5 million deaths worldwide. For the microbiological diagnosis of the disease, the most valid method is detecting the presence of the viral genome by real-time reverse transcription polymerase chain reaction (rRT-PCR). However, due to the nature of the RNA viruses, frequent mutations may affect the sensitivity of the analyses made on the genetic material of the virus, such as PCR. In this study, we aimed to investigate the mutations in the primer-probe binding regions of the rRT-PCR panels used in COVID-19 diagnosis. SARS-CoV-2 whole genome sequence data (n= 194) isolated from COVID-19 cases in Turkey and uploaded on GISAID database from the centers in Istanbul (n= 78), Ankara (n= 58), Kars (n= 47), Bursa (n= 2), Adiyaman (n= 2), Erciyes (n= 1) and Kocaeli (n= 1) between March 17-September 14, 2020 were analyzed. In order to determine the nucleotide changes, SARS-CoV-2 sequences from Turkey were compared to the reference genome sequence (NC_045512.1) present in GenBank website. The constructed data set was aligned using the MAFFT program and was checked manually if the sequences were in the same frame by using the AliView program. Primer-probe binding sites of the thirteen SARS-CoV-2 rRT-PCR panels from seven different institutes (US CDC, China CDC, Charite CDC, Pasteur, HKU, Thailand, NIID) that are being used in COVID-19 diagnosis were evaluated in terms of nucleotide changes within the corresponding regions compared to the reference genome. Sequence diversities in the viral genomes were determined via positional nucleotide numerical calculator and entropy calculator modules and nucleotide and entropy changes in primer-probe binding regions for each rRT-PCR panel were examined. Among thirteen different primer-probe panels, nucleotide changes in the target regions of the seven primer-probe panels were determined. When viral sequences with nucleotide changes in the primer-probe binding regions were examined, the most common changes were observed in the China CDC N-forward primer and US CDC N3-forward primer binding regions. It is important that the kits to be used as diagnostic tests are designed specific to the regions with less nucleotide changes. Nucleotide changes may not be critical for DNA amplification for most PCR panels, but should be carefully monitored as they may affect the sensitivity of the assay. If the risk of alteration of the designed region is high, the primer - probe binding sites should be checked frequently and updated when necessary.
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    Article
    Citation - WoS: 7
    Citation - Scopus: 11
    The Effect of Virtual Laboratory Simulations on Medical Laboratory Techniques Students' Knowledge and Vocational Laboratory Education
    (Walter De Gruyter Gmbh, 2022) Keles, Didem; Bulgurcu, Alihan; Demir, Esra Feyzioglu; Şemin, Makbule İlgi
    Objectives Virtual laboratory simulations (VLSs) are computer-based tools that offer unlimited application options in scientific, medical, and engineering fields. The aim of this study was to evaluate whether VLSs are efficient learning tools and how these simulations can be integrated into laboratory practice in medical laboratory education. Methods In this pre-test/post-test control group study, 32 volunteers were randomly assigned to either experimental or control groups. The experimental group performed laboratory simulations based on biochemistry and microbiology and then completed a self-report survey to evaluate their satisfaction and beliefs about simulations. Results In the experimental group, post-test scores of each simulation were significantly elevated compared to pre-test scores; however, pre- and post-test scores of control group were statistically the same. The experimental group agreed that these simulations should be applied before theoretical lectures and laboratory practices. They also highlighted that translating from English to their native language creates difficulties in applying and understanding the simulation. Conclusions We emphasized that VLSs are excellent learning tools that increase not only the knowledge but also the self-motivation and focus of the students. Based on feedbacks, native language options are necessary to enable the students to achieve equality of opportunity in education.
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    Conference Object
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    Article
    Validation of a Multiplex Qrt-PCR Assay for the Detection of RSV, Influenza A/B Virus and SARS-CoV
    (BMC, 2026) Bulgurcu, Alihan; Sayiner, Ayca Arzu
    The significant burden of viral respiratory diseases necessitates rapid detection of key pathogens. Simultaneous testing for SARS-CoV-2, RSV, and influenza A/B as an initial step, followed by broader panels as needed, offers a cost-effective diagnostic strategy. This study aimed to validate a new commercial multiplex qRT-PCR assay (Diagnovital (R) RTA Laboratories, Turkey) for the simultaneous detection of these viruses. Analytical sensitivity was determined using Probit regression analysis on serial dilutions (10(5)-10(1) copies/ml) for each target virus. Specificity was evaluated with 120 negative samples and 32 positive samples for non-target respiratory viruses. External quality control panels and clinical specimens positive for RSV (n = 39), influenza A/B (n = 71), SARS-CoV-2 (n = 64) were used for accuracy testing. Intra- and inter-assay precision were analyzed using samples near the limit of detection. The performance was compared to routine diagnostic tests. The assay's analytical sensitivity was 420.7, 296.7, 368.6, 1362.6, and 1459.7 copies/ml for SARS-CoV-2 Alpha and Omicron variants, influenza A, influenza B, and RSV, respectively. Analytical specificity was 100%, and precision showed CV% < 5. Detection rates for SARS-CoV-2, influenza A, influenza B, and RSV were 100%, 95.1%, 97.5%, and 94.8%, respectively, with false negatives occurring in samples with Ct > 33. Comparative analysis showed high correlations between assays, with strong agreement (Cohen's kappa ranging from 0.861 to 1). These findings demonstrate the clinical applicability of the Diagnovital (R) assay, though false negatives may occur in low-concentration samples.