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https://hdl.handle.net/20.500.14365/3643
Title: | Demonstration of G-Quadruplex Hybridization Chain Reaction for Nucleic Acid Detection | Authors: | Kanat B. Portakal, Hüseyin Saygın Doluca, Osman |
Keywords: | G-quadruplexes hybridization chain reaction nucleic acid detection signal amplification Biomedical engineering Diagnosis DNA DNA sequences Fluorescence Probes Amplex red Detection limits G-quadruplexes Hybridization chain reactions Nucleic acid detection Single-stranded DNA Polymerase chain reaction |
Publisher: | Institute of Electrical and Electronics Engineers Inc. | Abstract: | In recent years, the hybridization chain reaction (HCR) has been proposed as an alternative to polymerase chain reaction (PCR) for diagnosis. Unfortunately, the sensitivity of the HCR methods are still far below PCR and researchers focus on ways to improve it by investigating different HCR designs. While earlier designs exploit fluorescently labelled probes for detection, here we propose an HCR system that combines G-quadruplex formation and fluorescence for detection of single stranded DNA sequences with concentrations as low as 20 pM. We show that Gquadruplex-mediated oxidation of Amplex Red results in fluorescence increase and lowers the detection limit by about 10fold, in comparison to HCR using only fluorescently labelled HCR probes. © 2020 IEEE. | Description: | 2020 Medical Technologies Congress, TIPTEKNO 2020 -- 19 November 2020 through 20 November 2020 -- 166140 | URI: | https://doi.org/10.1109/TIPTEKNO50054.2020.9299304 https://hdl.handle.net/20.500.14365/3643 |
ISBN: | 9.78E+12 |
Appears in Collections: | Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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