Browsing by Author "Akhan, Sila"

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    Citation - WoS: 1
    Citation - Scopus: 1
    Evaluation of Lateral Flow and Elisa Techniques for Detecting Igg and Igm Antibodies in Covid-19 Cases in Turkiye
    (Who Eastern Mediterranean Regional Office, 2023) Arikan, Ayse; Doluca, Osman; Akhan, Sila; Sanlidag, Tamer; Sayan, Murat
    Background: Antibody testing can complement molecular assays for detecting COVID-19.Aims: We evaluated the concurrence between lateral flow assay and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).Methods: The study was conducted at Kocaeli University, Turkiye. We used a lateral flow assay and ELISA to test serum samples from COVID-19 cases, confirmed by polymerase chain reaction assays (study group) and pre-pandemic stored serum samples (control group). We used Deming regression to evaluate the antibody measurements.Results: The study group included 100 COVID-19 cases, and the control group included pre-pandemic samples from 156 individuals. The lateral flow assay detected immunoglobulin M (IgM) and G (IgG) antibodies in 35 and 37 study group samples. ELISA detected IgM nucleocapsid (N) antibodies in 18 samples, and IgG (N) and IgG spike 1 (S1) antibodies in 31 and 29 samples, respectively. None of the techniques detected antibodies in the control samples. Strong correlations were found between lateral flow IgG (N+ receptor-binding domain + S1) and ELISA IgG (S) (r = 0.93, P < 0.01) and ELISA IgG (N) (r = 0.81, P < 0.01). Weaker correlations were seen between ELISA IgG S and IgG N (r = 0.79, P < 0.01) and lateral flow assay and ELISA IgM (N) (r = 0.70, P < 0.01).Conclusion: Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult.
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    Citation - WoS: 3
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    Investigation of Compatibility of Severe Acute Respiratory Syndrome Coronavirus 2 Reverse Transcriptase-Pcr Kits Containing Different Gene Targets During Coronavirus Disease 2019 Pandemic
    (Future Medicine Ltd, 2020) Sarigul, Figen; Doluca, Osman; Akhan, Sila; Sayan, Murat
    Aim:In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.Materials & methods:Samples were tested by Bio-Speddy(R)(RdRpgene) and Diagnovital(R)(RdRp + Egenes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.Results:Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.Conclusion:We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.