Browsing by Author "Alpay, Suheda"

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Citation - WoS: 9
Citation - Scopus: 9
Classical Heart Rate Variability and Non-Linear Heart Rate Analysis in Mice Under Na-Pentobarbital and Ketamine/Xylazine Anesthesia
(Tubitak Scientific & Technical Research Council Turkey, 2022) Kazdagli, Hasan; Ozel, Hasan Fehmi; Ozbek, Mustafa; Alpay, Suheda; Alenbey, Muruvvet
Background/aim: Anesthetics are often used in animal experiments to achieve immobilization and relieve pain. However, many anesthetics can alter the dynamics of cardiovascular systems. We aimed to compare the effects of two frequently used anesthetics agents on heart rate variability (HRV) parameters in mice. Materials and methods: This observational study was performed between May and June 2014 in 21 male BALB/c mice aged 16-20 weeks. The animals were divided into three groups: pentobarbital (P), (n = 7); pentobarbital+fentanyl (P+ F), (n = 7); and ketamine+xylazine (K+X), (n = 7). Surface electrocardiography (ECG) electrodes were placed in lead II configuration. 'the tachogram of RR intervals was obtained after R waves were detected using the Pan-Tompkins real-time QRS recognition algorithm. Frequency-domain, time-domain, and nonlinear HRV analyses were performed. Results: The bradycardia effect was higher in the K+X group (p < 0.01). Time-domain indices were higher in group K+X compared to group P (p < 0.01) and group P+F (p < 0.001). Very low frequency (VLF) power was significantly lower in group K+X compared to group P and group P+F (p < 0.01). Low frequency (LF) power, low frequency/high frequency (LF/HF) ratio, and total power (TP) were higher in group K+X compared to group P (p < 0.01) and group P+F (p < 0.001). The detrended fluctuation analysis short-term parameter (DFA alpha(1)) was significantly higher in group K+X compared to group P+F (p < 0.05) and the long-term parameter (DFA alpha(2)) was lower in group K+X compared to group P (p < 0.05). Standard deviations SD1 and SD2 were higher in group K+X compared to group P (p < 0.001) and group P+F (p < 0.001), SD2/SD1 ratio was lower in group K+X compared to group P (p < 0.05) and group P+F (p < 0.05). Entropy measures did not differ between groups. Conclusion: HRV analyses, including nonlinear methods, indicated that a K+X combination reduces imbalance and disorder in the regulation of the autonomic nervous system (ANS) in comparison to both P and the P+F combination.
Evaluation of Differential Effects of Cdp-Choline and Choline on Parasympathetic Activity and Changes in Choline Levels With Heart Rate Variability
(Marmara univ, fac medicine, 2024) Kazdağlı, Hasan; Alpay, Suheda; Özel, Hasan Fehmi; Barış, Elif
Objective: Heart rate variability (HRV) is used to evaluate the autonomic activity of heartbeat. This study aimed to investigate the effects of cholinomimetic drugs cytidine diphosphate-choline (CDP-choline) and choline, on short-term HRV parameters. Materials and Methods: Animals were randomized into three groups; control (0.9% NaCl), choline (100 mg/kg), CDP-choline (400 mg/kg). Electrocardiography recordings were obtained for 45-minutes after treatments with 15-minutes intervals. HRV analyses and total choline level measurements in serum and heart tissues were performed. Results: High frequency power and total power increased in treatment groups, while heart rates were decreased. Low frequency was decreased with choline while very low frequency power decreased with CDP-choline. Choline affected most of the HRV parameters in the first 15 minutes, while the effect of CDP-choline started within 30 minutes. Total choline levels were higher in both treatment groups than in the control while the levels were also higher in the choline group compared to CDP-choline group. Conclusion: This study showed that CDP-choline and choline treatments produced a rapid response to short-term HRV parameters, while increasing tissue choline levels. Moreover, the differences in effects and onset time between the drugs on HRV might be related to tissue choline concentration.
Citation - WoS: 2
Citation - Scopus: 3
Sglt-2 Inhibitors on Cardiac Autonomic Function in Individuals With and Without Type 2 Diabetes Mellitus
(Elsevier Science inc, 2025) Ozel, Hasan Fehmi; Alpay, Suheda; Asker, Emre; Gultekin, Elif Sidal; Kazdagli, Hasan
Sodium-glucose cotransporter-2 (SGLT-2) inhibitors have emerged as key therapeutic agents in managing type 2 diabetes mellitus (T2DM) and obesity, offering benefits that extend beyond glycemic control. This review examines the role of SGLT-2 inhibitors in modulating cardiac autonomic function, with a particular focus on heart rate variability (HRV) as a biomarker of autonomic balance. These agents improve metabolic profiles through enhanced glucosuria, natriuresis, and weight loss, while concurrently reducing blood pressure. Importantly, they also attenuate sympathetic nervous system overactivity and promote parasympathetic modulation, which may lower the risk of adverse cardiovascular events. The underlying mechanisms include not only the metabolic effects but also anti-inflammatory and antioxidative actions, which together contribute to improved endothelial function and vascular health. Advanced HRV analyses, encompassing traditional time and frequency domain methods as well as nonlinear approaches, have proven valuable in detecting early autonomic dysfunction in high-risk populations. Some studies suggest that SGLT-2 inhibitors may be associated with improvements in HRV parameters, such as increased SDNN and RMSSD and a reduced LF/HF ratio. However, findings are inconsistent across studies, and further research is needed to determine the extent and mechanisms of these potential effects. Although these findings are promising, further standardized, long-term studies are essential to clarify the mechanisms and optimal therapeutic strategies involving SGLT-2 inhibitors in the management of autonomic dysfunction. Future research should also explore the synergistic potential of combining SGLT-2 inhibitors with other cardiometabolic therapies to enhance cardiovascular outcomes in individuals with and without T2DM.