Browsing by Author "Eroğlu, Hüseyin Avni"

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Demir Yükü Artmış Karaciğerde İnce İğne Aspirasyon Sitolojisi (iias) ve Tru-cut Biyopsisi: Deneysel Bir Çalışma
(2023) Adalı, Yasemen; Eroğlu, Hüseyin Avni; Makav, Mustafa; Karayol, Sunay Sibel; Fındık Güvendi, Gülname; Gök, Mustafa
Amaç: İnce iğne aspirasyon sitolojisi (İİAS) tru-cut biyopsiye göre daha güvenli, basit, hızlı ve uygun maliyetlidir ve hasta uyumu genellikle daha iyidir. Birçok organda kullanılan İİAS’nin karaciğerdeki parankimal hastalıklarda kullanımına ilişkin fazla veri yoktur. Bu nedenle bu çalışmada İİAS’nin demir birikimini ve karaciğer hasarını göstermedeki faydası değerlendirilmiştir. Yöntem: Çalışmada 2 kontrol grubu yer aldı. Çalışma sonunda herhangi bir uygulama yapılmayan bu gruplardan İİAS ve tru-cut biyopsi örnekleri alındı. Yirmi sekiz gün süreyle intraperitoneal demir dekstran (88 mg/kg) verilerek demir yüklenmesi oluşturulduktan sonra çalışma gruplarına İİAS ve tru-cut biyopsi işlemleri de yapıldı. Preparatlarda hepatosit hasarı, enflamasyon ve demir birikimi histopatolojik olarak değerlendirildi. Hepatektomi materyaline göre her iki yöntemin duyarlılığı, özgüllüğü, pozitif ve negatif prediktifliği ve tanısal doğruluğu hesaplandı. Bulgular: Hepatosit hasarında; sensitivite (%60), tanısal doğruluk (%66,7) ve negatif öngörü (%50) tru-cut grubunda anlamlı olarak daha yüksek bulundu. Enflamasyonun değerlendirilmesinde yöntemlerin birbirine üstün olmadığı ve İİAS’nin demir birikiminde %83,3 duyarlılık ve tanısal doğrulukla tru-cut biyopsi oranlarına (%100) neredeyse yaklaştığı görüldü. Sonuç: Demire bağlı karaciğer hasarında altın standart doku incelemesi olmalı, bu nedenle tru-cut biyopsi ilk seçenek olmalıdır. Ancak mevcut çalışma sonuçlarına dayanarak tru-cut biyopsinin kullanılamadığı kanamaya yatkınlık, cerrahi erişimin mümkün olmaması, eşlik eden farklı hastalıkların varlığı ya da hasta oryantasyonu ve kooperasyonunun zayıf olduğu durumlarda İİAS’nin daha invaziv seçeneklere göre tercih edilebileceği önerilmektedir.
Evaluation of Blood and Cerebrospinal Fluid Biochemistry, Cytology and Haematological Parameters in Head-And Form of Malignant Catarrhal Fever in Cattle
(2023) Uzlu, Erdoğan; Erkılıç, Ekin Emre; Adalı, Yasemen; Öğün, Metin; Can Şahna, Kezban; Eryeğen, Nilhan; Eroğlu, Hüseyin Avni
In this study, it was aimed to examine the biochemical changes, hematological changes and cerebrospinal fluid (CSF) cytology and blood serum of cattle with head-eye form of Malignant Catarrhal Fever (MCF). For this pur- pose, 22 cattle diagnosed with "head-eye form" of MCF and clinically healthy 10 cattle were evaluated. Blood and cere- brospinal fluid (CSF) were collected from all cattle. In sera, AST, urea, glucose, CK (P<0.05), LDH levels (P<0.01) were found be high, ALT, ALP, cholesterol (P<0.05), Ca, total protein (P<0.01) and Mg, albumine and Fe levels (P<0.001) were found to be low in MCF group when compared to the control group. In CSF, Ca (P<0.01) and total protein levels (P<0.001) were found high glucose level (P<0.05) was found low in MCF group when compared to the control group. In haematology, some parameters were determined to be different between the groups. In cytological results of CSF in MCF group, polymorphonuclear leucocytes, lymphocytes, erytrocytes, macrophages and plasma cells were determined. In conclusion, since there were a limited number of studies examining biochemical, cytologic and hematological results of MCF especially in CSF, the results from our study were thought to be important for future stud- ies in which viral diseases affects the nervous system of cattles.
Experimental Intravaginal and Intrauterine Endometritis Model: Which Model Is More Useful?
(2022) Beşeren, Hatice; Makav, Mustafa; Kuru, Mushap; Adalı, Yasemen; Coşkun, Mustafa Reha; Eroğlu, Hüseyin Avni
This study aims to compare the newly created intravaginal endometritis model (IVM) with the intrauterine endometritis model (IUM). E. coli infusion was used as intravaginally for IVM and intrauterinally for IUM model. The animals were exeuted on the 7th day. Histopathological and biochemical analyses [malondialdehyde (MDA), glutathione (GSH), Endocan, Endoglin] were performed. A significant inflammation was determined in IVM and IUM compared to the control. A significant decrease in GSH and a significant increase in MDA and Endoglin were determined in IVM and IUM compared to the control. There was a statistically significant increase in the IUM and a numerical increase in the IVM compared to the control. Endometritis was determined by histopathological and biochemical analyses in both IUM and IVM model. It is suggested that intravaginal administration, which is easier to perform, can be used in experimental endometritis model studies.
Investigation of the Effects of Dimethyl Sulfoxide in Experimental Gout With Comparison of Dexamethasone and Indomethacin
(Springer, 2024) Aydeğer, Cemre; Adalı, Yasemen; Makav, Mustafa; Eroğlu, Hüseyin Avni
Gout arthritis is an inflammatory arthritis characterized by increased serum uric acid and accumulation of monosodium urate (MSU) crystals in soft tissues. The treatment for gout arthritis is centered on reducing uric acid agents with long-term and anti-inflammatory agents during attack times. In recent studies, it is noteworthy that Indomethacin and Dexamethasone have positive effects in the treatment of gout. Dimethyl sulfoxide (DMSO) is a lipophilic solvent and has an anti-inflammatory effect at appropriate doses. Based on this information, for this study, the effects of these three agents were investigated in rats using a gut model to compare their efficacy. In the study, a total of 48 female 3-4-month rats were divided equally into 8 groups: Control, Indomethacin, DMSO, Dexamethasone, Gout, Gout+Indomethacin, Gout+DMSO, Gout +Dexamethasone. During the eight-week study, a gout arthritis model was used that included 10 mg MSU given intra-articularly in the right foot. Indomethacin 12.5 mg/kg intragastric, DMSO 0.1 ml intraperitoneally and dexamethasone 0.2 mg/kg were administered subcutaneously to the related groups once a day for seven days. At the end of the study, collected articular tissues were stained with haematoxylin and eosin after the fixation and decalcification processes were done. The findings obtained showed that inflammation was reduced in treatment groups compared to the Control groups (all p values 0.002). Also, synovial proliferation was remarkably decreased in the Gout+Dexamethasone group compared to the Gout group (p = 0.019). As a result of these findings, although the three agents all reduced inflammation in gout arthritis, DMSO was shown to be more advantageous due to its having fewer side-effects.