Browsing by Author "Gokahmetoglu, Selma"

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Evaluation of Alternative Clinical Samples for the Detection of SARS-CoV and Influenza Virus by Automated Multiplex RT-PCR
(J Infection Developing Countries, 2025) Ozarslan, Muhammed A.; Parkan, Omur M.; Soylu, Mehmet; Acet, Oguzhan; Gokahmetoglu, Selma; Yuce, Zeynep Ture; Erensoy, Selda
Introduction: The aim of this study was to compare the performance of different clinical specimens-nasopharyngeal (NP) swabs collected by healthcare professionals (HCP-NP), self-collected nasal swabs (Sc-N), and saliva samples (S)-in diagnostic tests for investigating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and influenza A/B RNA. Methodology: These clinical samples were collected from 404 symptomatic cases and tested with the SARS-CoV-2 and influenza A/B RNA tests on the cobas 6800 System of Roche Molecular Systems (Roche Molecular Systems, Pleasanton, USA). The SARS-CoV-2 or influenza virus infection status was determined for all patients based on the predefined criteria and corresponding algorithms. Positive and negative predictive values (PPV, NPV), sensitivity, specificity, coefficient of variation (CV), interrater reliability, correlation, ,and days of sample collection of these three sample types were analyzed. Results: There was almost perfect agreement between the these sample types for the diagnosis of SARS-CoV-2 and influenza A. The overall performance (PPV, NPV, sensitivity) and reproducibility (CV <= 6%) were favorable. Additionally, they showed similar trends for days of sample collection. Conclusions: Diagnostic detection of SARS-CoV-2 and influenza RNA from Sc-N and S samples was comparable to HCP-NP samples. Using these samples would provide an advantage in diagnosing SARS-CoV-2 and influenza A infection, as they can be easily collected without the need for viral transport media.
Citation - WoS: 1
Citation - Scopus: 1
Healthcare-Related Hcv Genotype 4d Infections in Kayseri, Turkey
(Aves, 2022) Gokahmetoglu, Selma; Polat, Ceylan; Atalay, Mustafa Altay; Sezgin, Gulten Can; Ergor, Gul; Aygen, Bilgehan; Gursoy, Sebnem; Abacıoğlu, Yusuf Hakan
Background: The frequency of genotype 4 hepatitis C virus infection is significantly higher in a city compared to other provinces in Turkey. In this study, we aimed to investigate the epidemiology and risk factors of hepatitis C virus genotype 4 infection in Kayseri province of Turkey. Methods: A case-control study was conducted with 61 hepatitis C virus genotype 4-infected patients and 71 controls. A questionnaire was administered to the patients and controls, asking for information about the risk factors of hepatitis C virus transmission. Core/E1 and NS5B regions of hepatitis C virus genome were amplified and sequenced by Sanger method. Phylogenetic analysis and molecular clock analysis were performed. The risk was determined by calculating the odds ratio and 95% CI. Logistic regression analysis was performed to determine the effect of risk factors by controlling for confounding variables. Results: Kayseri isolates were closely related to type 4d sequences but formed a separate cluster. According to the molecular clock analysis, hepatitis C virus genotype 4d entered Kayseri province probably between 1941 and 1988. Blood transfusion and surgical intervention were found to be significant risk factors for the infection. Conclusion: Epidemiological data showed that hepatitis C virus genotype 4d infections are significantly associated with unsafe medical procedures.