Browsing by Author "Hamurtekin, Emre"

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Cholinergic Receptor Binding Profile of Hypericum Perforatum L. and Its Active Constituents
(Asian Network Scientific Information-Ansinet, 2022) Hamurtekin, Emre; Hamurtekin, Y.; Matucci, R.; Dei, S.; Dayanç, Barış Emre; Kazdağlı, Hasan
Background andObjective: Hypericum perforatum L (HP) is a popular herbal medicine with different pharmacological effects. This study investigated the possible cholinergic receptor affinities of HP extract and its three active constituents: hyperforin, hypericin and pseudohypericin. Materials and Methods: Radioactive compounds [3H]-N-methyl scopolamine used for muscarinic receptor binding studies in Chinese hamster ovary cells expressing human muscarinic receptor subtypes and [3H]-cytisine used for nicotinic receptor binding tests performed with mouse brains without a cerebellum. Muscarinic binding inhibition was observed with HP extract considerably for hM2 and hM5. Results: Hyperforin, hypericin and pseudohypericin showed a much lower affinity for muscarinic receptors at higher concentrations. The HP extract and its constituents did not produce any nicotinic receptor binding inhibition. Conclusion:These results suggested that post-junctional direct muscarinic receptor interaction may modulate some effects of HP extract and its constituents however different mechanisms apart from direct cholinergic receptor interaction might be considered for the pharmacological actions of hyperforin, hypericin and pseudohypericin.
Effect of Choline and Cdp-Choline on Inflammation and Oxidative Stress in Burkitt's Lymphoma Cells
(Asian Network Scientific information-ansinet, 2025) Roshani, Shideh; Baris, Elif; Bosnak, Ahmet Sami; Gali-Muhtasib, Hala; Hamurtekin, Emre
Background and Objective: Burkitt's lymphoma (BL) is a specific type of non-Hodgkin lymphoma. The BL is characterized by rapid progression and a tendency to metastasize the bone marrow and central nervous system. This study aims to evaluate the anticancer potential of choline and CDP-choline on BL cells (Ramos cells), in vitro. Materials and Methods: Ramos cells were treated with increasing concentrations of doxorubicin, choline and CDP-choline for 24 hrs after which cell viability was assessed using the MTT assay. Cytokine levels (IL-6 and TNF-") and reactive oxygen species (ROS) production were measured using ELISA and fluorometric kits, respectively. One-way Analysis of Variance (ANOVA) with post hoc Tukey-Kramer multiple comparison tests were used for the statistical analysis, p<0.05 was accepted as a statistically significant level. Results: Choline and CDP-choline treatment for 24 hrs decreased Ramos cell viability, with IC50 values of 100, 02 and 5.45 M, respectively. Both treatments increased ROS levels, indicating induction of oxidative stress. However, treatment of Ramos cells with these agents for 24 hrs did not induce cytokines (IL-6 and TNF-") production. Choline treatment increased supernatant choline levels, whereas CDP-choline had no significant effect on intracellular choline in Ramos cells. Conclusion: Choline and CDP-choline reduced cell viability of Ramos cells probably via ROS dependent mechanism, but did not induce inflammatory responses at 24 hrs post-treatment.Thesefindings suggested the possible anticancer potential ofcholine and CDP-choline against BL. This warrants further investigation into their potential therapeutic implications.
Citation - WoS: 8
Effects of Cdp-Choline and Choline on Cox Pathway in Lps-Induced Inflammatory Response in Rats
(Asian Network Scientific Information-Ansinet, 2021) Barış, Elif; Simsek, O.; Efe, H.; Oncu, S.; Gelal, A.; Hamurtekin, Emre; Tosun, M.
Background and Objective: Cytidine-5-diphosphate-choline (CDP-choline) and choline activate the cholinergic anti-inflammatory pathway in case of inflammation. This study investigated the role of CDP-choline and choline along with the contribution of the cyclooxygenase (COX) pathway on the lipopolysaccharide (LPS)-induced endotoxemia model in rats. Materials and Methods: Endotoxemia model was induced by LPS administration. CDP-choline or choline 5 min before and 6 hrs after LPS injection. The sepsis severity, body weight changes, survival rate were evaluated. Serum prostaglandins, Tumour Necrosis Factor (TNF)-alpha, total choline levels were measured. COX-2 mRNA expression and protein levels were analyzed. Spleen tissues were evaluated histomorphological. One-way analysis of variance analysis (ANOVA) or Kruskal Wallis tests was used for statistical analysis. Results: COX-2 expressions in liver and brain tissues, serum prostaglandin E-2, 6-keto prostaglandin F-1 alpha, Thromboxane A(2) and TNF alpha levels were increased 24 hrs after LPS administration. Administrations of CDP-choline or choline were decreased COX-2 expression in the liver. Serum prostaglandin levels were decreased in the CDP-choline-treated group, whereas, only prostaglandin E-2 level was decreased in the choline-treated group. Total choline levels in serum and brain were increased after CDP-choline or choline administration. Accordingly, serum TNF alpha levels and TNF alpha expression in the liver were decreased in CDP-choline and choline-treated groups. TNF alpha expression in the brain was decreased in the choline-treated group, whereas, increased in the CDP-choline-treated group. Conclusion: CDP-choline and choline decreased LPS-induced COX-2 enzyme expression and prostaglandin levels in the periphery by increasing serum and brain total choline levels in the LPS-induced endotoxemia model in rat.
Citation - WoS: 1
The Role of Nicotinic Anti-Inflammatory Pathway in Prostaglandin Mediated Inflammatory Response in Sepsis: a Short Review
(Marmara Univ, Inst Health Sciences, 2019) Baris, Elif; Arici, Mualla Aylin; Hamurtekin, Emre
Sepsis is a severe and multifaceted condition of body in response to an infection, which affects multiple organs systems that makes it difficult to treat and enhances the mortality rates. Release of inflammatory cytokines can initiate an inflammatory response during sepsis. However, the response can be modified by the control mechanism inside the body that are essential for the keeping the balance and survival. The cholinergic anti-inflammatory pathway is defined as a comprehensive neurohumoral pathway that diminishes pro-inflammatory cytokine release through the vagus nerve and cholinergic receptors, predominantly alpha 7 nicotinic acetylcholine receptors (alpha 7nAChR) that expressed on inflammatory mononuclear cells. Thus, cholinergic agonists might be a part of prospective treatment approach in inflammatory diseases such as sepsis. This review covers the role of cholinergic system in prostaglandin mediated inflammatory response.
Citation - WoS: 2
Citation - Scopus: 3
Serum Choline, Leptin and Interleukin-6 Levels in Fibromyalgia Syndrome-Induced Pain: a Case-Control Study
(Bmc, 2025) Baris, Elif; Topaloglu, Izel; Akalin, Elif; Hamurtekin, Emre; Kabaran, Seray; Gelal, Ayse; Arici, Mualla Aylin
Background Fibromyalgia Syndrome (FMS) predominantly affects middle-aged women, characterized by musculoskeletal pain, fatigue, and cognitive issues. Choline, an endogenous molecule, may influence FMS due to its analgesic and anti-inflammatory properties. This study compared choline, leptin, and interleukin-6 (IL-6) levels in FMS patients and controls and examining their association with pain severity. Methods Volunteers with FMS were clinically diagnosed at a Physical Medicine and Rehabilitation Department. The control group included pain-free volunteers. Pain severity was gauged using a numeric scale, dietary choline intake through a questionnaire. Serum choline, leptin and (interleukin)IL-6 levels were measured from fasting blood samples of volunteers with enzyme-linked immunosorbent assays (ELISA). Results All FMS patients (n = 38) and healthy volunteers (n = 38) were female. Pain score in patients with FMS was 7.6 +/- 0.2. Dietary choline intake was lower in patients with FMS than the controls (p = 0.036). Serum choline and leptin levels were lower in the FMS group compared to control (p = 0.03). Serum IL-6 levels were higher in the FMS group than in the control (p < 0.001). There was weak positive correlation between IL-6 levels and pain scores and there were no correlation between leptin levels and pain scores in FMS. Conclusions This research highlights FMS's complex nature, involving neurochemical, immunological, and nutritional factors. It suggests the significance of choline's anti-inflammatory effect, leptin's metabolic function, and IL-6's role in FMS pathology. The results suggest that reduced dietary choline might influence serum choline, leptin, and IL-6 levels, potentially impacting FMS-related pain. This points to the potential of supplementary choline intake in FMS management. Trial registration Not applicable (Non-interventional study).