Browsing by Author "Koruyucu, Meryem"

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Citation - WoS: 6
Citation - Scopus: 6
Dna Isolation by Galactoacrylate-Based Nano-Poly(hema Nanopolymers
(Taylor & Francis Ltd, 2017) Kayhan, Ceren Turkcan; Ural, Fulden Zeynep; Koruyucu, Meryem; Salman, Yesim Gul; Uygun, Murat; Uygun, Deniz Aktas; Akgol, Sinan
Isolation of DNA is one of the important processes for biotechnological applications such as investigation of DNA structures and functions, recombinant DNA preparations, identification of genetic factors and diagnosis and treatment of genetic disorders. The aim of this study was to synthesis and characterizes the galactoacrylate based nanopolymers with high surface area and to investigate the usability of these synthesized nanopolymers for DNA isolation studies. Nanopolymers were synthesized by the surfactant free emulsion polymerization technique by using the monomers of 2-hydroxyl ethylmethacrylate and 6-O-(2'-hydroxy-3'-acryloyloxypropyl)-1,2:3,4-di-O-isopropylidene-alpha-D-galactopyranose. Galactoacrylate origin of these newly synthesized nanopolymers increased the interaction between DNA and nanopolymers. Prepared nanopolymers were characterized by SEM, FT-IR and ZETA sizer analysis. Synthesized nanopolymers were spherical, and their average particle size was about 246.8nm. Adsorption of DNA onto galactoacrylate based nanopolymers was investigated by using different pHs, temperatures, ionic strength, DNA concentrations and desorption studies and maximum DNA adsorption was found to be as 567.12 mg/g polymer at 25 degrees C, in pH 5.0 acetate buffer. Reusability was investigated for 5 successive reuse and DNA adsorption capacity decreased only about 10% at the end of the 5th reuse.
Citation - WoS: 11
Citation - Scopus: 13
Mannose Based Polymeric Nanoparticles for Lectin Separation
(Taylor & Francis Inc, 2018) Demir, Esra Feyzioglu; Atay, Nevra Ozturk; Koruyucu, Meryem; Kok, Gokhan; Salman, Yesim; Akgol, Sinan
The aim of this work is to synthesize the original, new polymeric nanoparticles for concanavalin A (Con A) purification. Nanoparticles were synthesized by surfactant free emulsion polymerization. In the polymerization prosedure, 1-O-(2'-hydroxy-3'-acryloyloxypropyl)-2,3:5,6-di-O-isopropylidene-alpha-D-mannofuranose (Man-OPA) was used as co-monomer and 2-hydroxyethylmethacrylate (HEMA) was used as a monomer. Man-OPA was characterized by Fourier Transform Infrared Spectroscopy (FTIR), nuclear magnetic resonance and elemental analysis techniques. Poly(HEMA-Man-OPA) nanoparticles were characterized by scanning electron microscopy, FTIR and Zeta Sizer. In adsorption-desorption experiments, maximum Con A adsorption capacity of poly(HEMA-Man-OPA) nanoparticles was found 630.6 mg/g nanoparticle (pH 7.5, 1.0 mg/mL). Adsorption-desorption experiments were repeated in four times. According to results, these nanoparticles could be used several times without significant decrease in Con A adsorption capacity.