Browsing by Author "Makav, Mustafa"

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Demir Yükü Artmış Karaciğerde İnce İğne Aspirasyon Sitolojisi (iias) ve Tru-cut Biyopsisi: Deneysel Bir Çalışma
(2023) Adalı, Yasemen; Eroğlu, Hüseyin Avni; Makav, Mustafa; Karayol, Sunay Sibel; Fındık Güvendi, Gülname; Gök, Mustafa
Amaç: İnce iğne aspirasyon sitolojisi (İİAS) tru-cut biyopsiye göre daha güvenli, basit, hızlı ve uygun maliyetlidir ve hasta uyumu genellikle daha iyidir. Birçok organda kullanılan İİAS’nin karaciğerdeki parankimal hastalıklarda kullanımına ilişkin fazla veri yoktur. Bu nedenle bu çalışmada İİAS’nin demir birikimini ve karaciğer hasarını göstermedeki faydası değerlendirilmiştir. Yöntem: Çalışmada 2 kontrol grubu yer aldı. Çalışma sonunda herhangi bir uygulama yapılmayan bu gruplardan İİAS ve tru-cut biyopsi örnekleri alındı. Yirmi sekiz gün süreyle intraperitoneal demir dekstran (88 mg/kg) verilerek demir yüklenmesi oluşturulduktan sonra çalışma gruplarına İİAS ve tru-cut biyopsi işlemleri de yapıldı. Preparatlarda hepatosit hasarı, enflamasyon ve demir birikimi histopatolojik olarak değerlendirildi. Hepatektomi materyaline göre her iki yöntemin duyarlılığı, özgüllüğü, pozitif ve negatif prediktifliği ve tanısal doğruluğu hesaplandı. Bulgular: Hepatosit hasarında; sensitivite (%60), tanısal doğruluk (%66,7) ve negatif öngörü (%50) tru-cut grubunda anlamlı olarak daha yüksek bulundu. Enflamasyonun değerlendirilmesinde yöntemlerin birbirine üstün olmadığı ve İİAS’nin demir birikiminde %83,3 duyarlılık ve tanısal doğrulukla tru-cut biyopsi oranlarına (%100) neredeyse yaklaştığı görüldü. Sonuç: Demire bağlı karaciğer hasarında altın standart doku incelemesi olmalı, bu nedenle tru-cut biyopsi ilk seçenek olmalıdır. Ancak mevcut çalışma sonuçlarına dayanarak tru-cut biyopsinin kullanılamadığı kanamaya yatkınlık, cerrahi erişimin mümkün olmaması, eşlik eden farklı hastalıkların varlığı ya da hasta oryantasyonu ve kooperasyonunun zayıf olduğu durumlarda İİAS’nin daha invaziv seçeneklere göre tercih edilebileceği önerilmektedir.
Experimental Intravaginal and Intrauterine Endometritis Model: Which Model Is More Useful?
(2022) Beşeren, Hatice; Makav, Mustafa; Kuru, Mushap; Adalı, Yasemen; Coşkun, Mustafa Reha; Eroğlu, Hüseyin Avni
This study aims to compare the newly created intravaginal endometritis model (IVM) with the intrauterine endometritis model (IUM). E. coli infusion was used as intravaginally for IVM and intrauterinally for IUM model. The animals were exeuted on the 7th day. Histopathological and biochemical analyses [malondialdehyde (MDA), glutathione (GSH), Endocan, Endoglin] were performed. A significant inflammation was determined in IVM and IUM compared to the control. A significant decrease in GSH and a significant increase in MDA and Endoglin were determined in IVM and IUM compared to the control. There was a statistically significant increase in the IUM and a numerical increase in the IVM compared to the control. Endometritis was determined by histopathological and biochemical analyses in both IUM and IVM model. It is suggested that intravaginal administration, which is easier to perform, can be used in experimental endometritis model studies.
Investigation of the Effects of Dimethyl Sulfoxide in Experimental Gout With Comparison of Dexamethasone and Indomethacin
(Springer, 2024) Aydeğer, Cemre; Adalı, Yasemen; Makav, Mustafa; Eroğlu, Hüseyin Avni
Gout arthritis is an inflammatory arthritis characterized by increased serum uric acid and accumulation of monosodium urate (MSU) crystals in soft tissues. The treatment for gout arthritis is centered on reducing uric acid agents with long-term and anti-inflammatory agents during attack times. In recent studies, it is noteworthy that Indomethacin and Dexamethasone have positive effects in the treatment of gout. Dimethyl sulfoxide (DMSO) is a lipophilic solvent and has an anti-inflammatory effect at appropriate doses. Based on this information, for this study, the effects of these three agents were investigated in rats using a gut model to compare their efficacy. In the study, a total of 48 female 3-4-month rats were divided equally into 8 groups: Control, Indomethacin, DMSO, Dexamethasone, Gout, Gout+Indomethacin, Gout+DMSO, Gout +Dexamethasone. During the eight-week study, a gout arthritis model was used that included 10 mg MSU given intra-articularly in the right foot. Indomethacin 12.5 mg/kg intragastric, DMSO 0.1 ml intraperitoneally and dexamethasone 0.2 mg/kg were administered subcutaneously to the related groups once a day for seven days. At the end of the study, collected articular tissues were stained with haematoxylin and eosin after the fixation and decalcification processes were done. The findings obtained showed that inflammation was reduced in treatment groups compared to the Control groups (all p values 0.002). Also, synovial proliferation was remarkably decreased in the Gout+Dexamethasone group compared to the Gout group (p = 0.019). As a result of these findings, although the three agents all reduced inflammation in gout arthritis, DMSO was shown to be more advantageous due to its having fewer side-effects.
Citation - WoS: 15
Citation - Scopus: 15
Selenium or Ozone: Effects on Liver Injury Caused by Experimental Iron Overload
(Pergamon-Elsevier Science Ltd, 2020) Guevendi, Guelname Frndrk; Eroglu, Hueseyin Avni; Makav, Mustafa; Guvendi, Buelent; Adalre, Yasemen
Aims: Iron is an important metal ion as a biocatalyst on the other hand iron overload causes various diseases. Iron overload can result in fibrosis and hepatocellular carcinoma with various pathophysiological mechanisms, including oxidative damage in the liver. Therefore; in this study the effects of ozone and selenium-whose antioxidant properties are known- were evaluated in liver injury induced by iron overload. Materials and methods: Iron overload model was provided by intraperitoneal administration of 88 mg/kg iron dextrate for 4 weeks. After iron dextran administration, ozone and selenium administrations were made for 3 weeks. From the obtained blood and tissue samples total oxidant status (TOS) and total antioxidant status (TAS) were determined and histopathological examination was performed in liver tissue samples. Key findings: In rats with iron overload, the lowest mean serum TOS was observed in the selenium administration group. The highest tissue TOS means and the lowest tissue TAS means were determined in the group in which ozone and selenium were administrated together. When histopathological data were evaluated, the presence of increased apoptosis in the ozone group compared to the iron group (p = 0.019) and selenium group (p= 0.019) was noted. Similarly, increased periportal inflammation (p = 0.001) and fibrosis (p = 0.005) were observed in the ozone group compared to the selenium group. Significance: In iron-induced liver damage, ozone was thought to be effective by decreasing ROS, but contrary to expectations, it was observed that it may negatively affect the picture by showing synergistic effect. However, the effects of selenium on both serum and tissue levels are promising.
Therapeutic Efects of Boric Acid in a Septic Arthritis Model Induced by Escherichia Coli in Rats (jan, 10.1007/S12011-021-03065-7, 2022)
(Springernature, 2023) Yildiz, Kadri; Makav, Mustafa; Adali, Yasemen; Bulut, Menekse
[Abstract Not Available]
Citation - WoS: 9
Citation - Scopus: 8
Therapeutic Effects of Boric Acid in a Septic Arthritis Model Induced by Escherichia Coli in Rats
(Springernature, 2022) Yildiz, Kadri; Makav, Mustafa; Adali, Yasemin; Bulut, Menekse
The study aimed to evaluate the therapeutic effect of boric acid (BA) in experimentally induced septic arthritis. A total of 30 rats, 6 rats in each group (5 groups), were used in the study. No treatment was applied to the rats in the control group. Only BA was administered intraperitoneally (IP) to the rats in the bor group. Escherichia coli was administered at a single dose of 25 mu L, 1 x 10(10) cfu/rat from the right foot pad of the rats, via intra-articular route, to the mice in the arthritis, arthritis-bor, and arthritis-antb groups. Then, BA at a dose of 50 mg/kg and cefazolin at a dose of 25 mg/kg were administered to the rats in the arthritis-bor and arthritis-antb groups, respectively, for 7 days via the IP route. At the end of the study, all animals were euthanized following the ethical rules. Blood and tissue samples were taken from the rats for biochemical and histopathological analyses. The levels of GSH, MDA, Endoglin, Endocan, and TNF-beta markers were measured in the blood samples taken. A significant decrease was observed in MDA and Endoglin levels in the boric acid-administered group compared with the arthritis group, while a significant increase was observed at the GSH level. Histopathologically, it was determined that the reactive surrounding tissue response in the bor group was significantly reduced. As a result, a significant decrease in inflammation was found biochemically and histopathologically in the groups treated with BA.