Browsing by Author "Micili, Serap Cilaker"

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Ionizing Radiation-Induced Testicular Oxidative Stress and Apoptosis: The Role of Small GTPase RhoA
(Taylor & Francis Ltd, 2025) Manisaligil, Yasar Aysun; Yurt, Aysegul; Ozkan, Cemre Ural; Micili, Serap Cilaker; Sisman, Gizem; Cavdar, Zahide; Gumustekin, Mukaddes
PurposeThe effects of ionizing radiation on living organisms are mainly known as the generation of reactive oxygen species (ROS), apoptosis, and DNA damage. Small GTPases (RhoA, Rac1, Cdc42) are known to have roles in the regulation of oxidative stress and apoptosis. The aim of this study was to investigate the role of the RhoA molecule in testicular tissue damage due to oxidative stress and apoptosis induced by ionizing radiation.Material and methodIn this study, testicular tissues and blood samples obtained from our previous study were examined. In that study, rats were exposed to ionizing radiation at three different doses (0.02 Gy, 0.1 Gy, 5 Gy). Then tissue and blood samples were taken at three different times (2 hours, 24 hours, and 7 days) after irradiation. Immunohistochemical staining was performed to evaluate RhoA and cleaved caspase-3 expressions, while RhoA activity was assessed by G-LISA assay in testicular tissues. Serum malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were analyzed to evaluate oxidative stress.ResultsThe expression and activation of RhoA demonstrated a time-dependent increase across all levels of radiation doses. Similarly, the expression of cleaved caspase-3 also exhibited a time-dependent increase, consistent with the effects of radiation-induced damage observed in all experimental groups. After exposure to radiation, serum levels of MDA increased, while the activity of SOD decreased.ConclusionOur findings suggest that RhoA may contribute to radiation-induced testicular tissue damage by increasing oxidative stress and apoptosis.
Citation - WoS: 16
Citation - Scopus: 18
The Role of Resveratrol on Full - Thickness Uterine Wound Healing in Rats
(Elsevier Taiwan, 2017) Sayin, Oya; Micili, Serap Cilaker; Goker, Asli; Kamaci, Gonca; Ergur, Bekir Ugur; Yilmaz, Osman; Akdoğan, Gül
Objective: Healing of the uterus after cesarean section and myomectomy operation is clinically important. In this study, we aimed to investigate the effects of resveratrol (3,5,4'-o-trihydroxystilbene) on the wound healing process of the uterus in rats treated with resveratrol following full thickness injury of the uterus. Materials and methods: Twenty-one female wistar albino rats were divided randomly into three groups (1) control group with no intervention (2) injury group with uterine full thickness injury (3) resveratrol group with uterine full thickness injury and treated with resveratrol. Resveratrol was injected by oral gavage at the doses of 0.5 mg/kg/day for 30 days following uterine full thickness injury. Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) distributions were assessed using the immunohistochemical methods in tissue and ELISA methods in the tissue homogenate. Glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were evaluated with colorimetric method and malondialdehyde (MDA) levels also were measured using high performance liquid chromatography in the tissue homogenate. The effects of resveratrol on the uterine histology also were evaluated histologically with the light microscopy. Results: Histological evaluation and immunohistochemical evaluations showed that treatment with a resveratrol significantly increased the thickness of the uterine wall and VEGF expression and decreased expression PDGF during wound healing. Biochemically, GPx and SOD activities were increased significantly after treatment with resveratrol. Additionally, resveratrol administration decreased MDA levels. Conclusion: These results showed that the antioxidant effects of resveratrol has been shown to have a positive influence on wound healing of the uterus. (C) 2017 Taiwan Association of Obstetrics & Gynecology. Publishing services by Elsevier B.V.