Browsing by Author "Mutaf, Tuğçe"

Now showing 1 - 4 of 4

Citation - WoS: 1
Citation - Scopus: 5
Biogenic Nanoparticle Synthesis Using Marine Alga Schizochytrium Sp.
(Springer, 2020) Mutaf, Tuğçe; Çalışkan, Gülizar; Meydan, Cafer; Oncel, Suphi Surisvan; Elibol, Murat
Nanotechnology is one of the most promising science and technology discipline that targets to bring new solutions for many applications in biotechnology, biomedical, energy and cosmetic industry by improving particles and devices scale of nanometers. Various sized and shaped nanoparticles can be synthesized by several methods. Up to now, scientists prefer physical and chemical fabrication of nanoparticles. But, these methods contain use of toxic, expensive and non-environmentalist solvents, reducing and stabilising agents. For a sustainable science, there is a necessity development of more eco-friendly, cost-effective and trustable alternative processes. In this context, using biological sources as reaction agent, have a strong potential. Plants, bacteria, fungi are essential biological sources for transformation of metals to nanoparticles. Many researchers focus on fungi and bacteriological potential in nanofabrication whereas algae are highly intriguing biological systems in nanotechnological approach. Some of cyanobacteria and algae have previously been used to synthesize intracellular or extracellular metal nanoparticles. Most of the research concentrate especially on gold and silver nanoparticle production from algae. In this work; bioreduction of silver, zinc and iron metals have been investigated using culture supernatant of marine algae Schizochytrium sp. For characterization of nanoparticles, UV visible spectroscopy, zeta sizer were used. Nanoparticle size was determined by zeta sizer and particles' surface plasmon resonance band detected by UV-Visible Spectroscopy.
Citation - WoS: 25
Citation - Scopus: 28
Green Synthesis and Characterization of Titanium Nanoparticles Using Microalga, Phaeodactylum Tricornutum
(Taylor & Francis Inc, 2022) Çalışkan, Gülizar; Mutaf, Tuğçe; Agba, Hasan Cenk; Elibol, Murat
Nanoparticles synthesized from microalgae offer a newly discovered process that is open to improvement. In this study supernatant of Phaeodactylum tricornutum (P. tricornutum) culture was used for this purpose. Firstly, the effects of some variables, namely titanium concentrations, titanium:supernatant ratio, pH, incubation time, and mixing speed on nanoparticle synthesis were investigated by using statistical design method in shaken culture as well as bioreactor. The average nanoparticle size synthesized in optimum conditions which were pH 7.5 with 300 rpm for 1 h was found as 50 nm. In the second part, nanoparticles were coated with a chitosan solution to protect their stability and increase their potential. Therefore, the antimicrobial activity showed a reasonable effect on these nanoparticles coated with chitosan. Nanoparticles produced had interestingly shown 99% antistatic properties. According to the cytotoxicity test, these nanoparticles showed a high cytotoxic effect on different cancer cell lines. The results obtained in the present study can be considered promising outcomes for possible future antimicrobial, biogenic and antistatic studies particularly in biomedical applications.
Citation - WoS: 13
Citation - Scopus: 20
Green Synthesis of Metal Nanoparticles Using Microalga Galdieria Sp.
(Springer, 2020) Çalışkan, Gülizar; Mutaf, Tuğçe; Oncel, Suphi Surisvan; Elibol, Murat
Green synthesis of nanoparticles has recently been a preferred method since it offers useful approaches such as non-toxic, biocompatible, environmentally friendly, cost-effective, stable product and trustable alternative processes compared to other methods. Various biological sources such as plant, algae, fungus and bacteria are widely used in biological synthesis of nanoparticles. Algae are more adapted organisms compared to the others for having high growth rate and biomass productivity, high heavy metal accumulation capacity, etc. Nanoparticles can be used in various area such as antimicrobial, antifungal, antioxidant agent, admixture of biosensor and drug transport/release, also diagnosis and treatment systems. Since each organism has different biochemical composition and metabolic pathways, their synthesized nanoparticles are going to show various characteristics and application area. In this study, silver, iron (II) and zinc nanoparticles were produced by using microalga Galdieria sp. The synthesized nanoparticles were characterized by using UV visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and zeta sizer. Antimicrobial activity against gram negative and gram-positive bacteria was also examined throughout the study. In conclusion, the potential of this biogenic nanoparticle was discussed.
Citation - WoS: 3
Metal Nanopartiküllerin Mikroalgler Aracılığı ile Yeşil Sentezi
(2023) Mutaf, Tuğçe; Çalışkan Bilgin, Gülizar; Öncel, Suphi; Elibol, Murat
Yeşil sentez olarak adlandırılan, nanopartiküllerin biyolojik kaynaklar aracılığı ile sentezlenmesine olan ilgi son yıllarda artış göstermiştir. Bunun temel nedeni geleneksel yöntemler olan fiziksel ve kimyasal yöntemlerde indirgeyici ve stabilize edici ajanlar olarak yüksek miktarlarda toksik kimyasala ihtiyaç duyuluyor olmasıdır. Daha çevre dostu ve insan sağlığı için tehdit oluşturmayan bitki, fungus, bakteri, alg gibi organizmalar yeşil nanopartikül sentezi için alternatif kaynaklardır. Sucul mikroorganizmalar olan mikroalgler üretmiş oldukları proteinler, vitaminler, pigmentler, yağ asitleri, hücre içi- hücre dışı polisakkaritler gibi fonksiyonel özelliğe sahip metabolitler sayesinde uzun yıllardır gıda, kozmetik ve ilaç endüstrilerinde formülasyonlara eklenmektedir. Bunların yanı sıra, son yıllarda yapılan çalışmalarla nanopartikül sentezinde de yüksek potansiyele sahip oldukları görülmüştür. Özellikle metal iyonlarının depolanmasını ve detoksifikasyonunu yapabildiklerinden ve metal iyonlarını elementel hale indirgeyen hücre içi ve hücre dışı metabolitlerce zengin olduklarından, metal nanopartiküllerin sentezi için yüksek potansiyele sahiptirler. Son yıllarda mikroalglerden nanopartikül sentezine odaklanmış olan yayın sayısı artmış ve pek çok mikroalg türünün gümüş, altın, titanyum, çinko, demir vb. metal nanopartikülleri hücre içi ve hücre dışı yollarla sentezleme potansiyeli araştırılmıştır. Bu derleme makale kapsamında, nanopartikül sentezi için çalışılmış olan mikroalg ve siyanobakteri türleri, kullanılan farklı sentez yöntemleri, nanopartiküllerin sentez mekanizması, temel karakterizasyon yöntemleri ve yeşil sentezle üretilen nanopartiküllerin antimikrobiyal aktivitelerine odaklanılmıştır