Browsing by Author "Ozbilgin, Kemal"

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Citation - WoS: 7
Citation - Scopus: 6
Distribution of Furin, Tnf-Alpha, and Tgf-Beta 2 in the Endometrium of Missed Abortion and Voluntary First Trimester Termination Cases
(Sci Printers & Publ Inc, 2015) Ozbilgin, Kemal; Turan, Afsin; Kahraman, Burcu; Atay, Coskun; Vatansever, Seda; Uluer, Elgin T.; Ozcakir, Tayfun
OBJECTIVE: To identify the role of furin, TNF-alpha, and TGF-beta 2 in human missed abortion pathogenesis. STUDY DESIGN: Decidual materials were collected from patients diagnosed with a missed abortion (n=10) (missed abortion group) and from legal voluntary termination cases at <10 gestational weeks (n=10) (normal pregnancy group). Tissue samples were collected from each group by dilation and curettage under mask anesthesia. For all tissue samples, furin, TNF-alpha, and TGF-beta 2 primary antibodies were performed by immunohistochemical staining. The number of stained cells was evaluated by using the H-score technique. RESULTS: In immunohistochemical examination, the immunoreactivities of furin, TNF-alpha, and TGF-beta 2 were found to be higher in syncytiotrophoblastic cells in the missed abortion group than in the normal pregnancy group (p < 0.005). Additionally, high immunoreactivity of TNF-alpha and TGF-beta 2 molecules was established only in cytotrophoblastic cells of missed abortions (p <0.005) in examination at decidual cells of the missed abortion group; furin immunoreactivities were detected higher in the missed abortion group than in the control group, but TNF-alpha and TGF-beta 2 immunoreactivity were increased in number in the normal pregnancy group (p < 0.005). CONCLUSION: It is considered that high levels of furin and the 2 furin-related proteins (TNF-alpha and TGF-beta 2), which play important roles in proliferation, invasion, migration, differentiation, and survival of cells, may be the reason of proceeding decidualization, placentation, and prevention from abortion, in spite of terminating the fetal life.
Citation - WoS: 2
Immunohistochemical Determination of Mtor Pathway Molecules in Ovaries and Uterus in Rat Estrous Cycle Stages
(F Hernandez, 2020) Ekizceli, Gulcin; Inan, Sevinc; Oktem, Gulperi; Onur, Ece; Ozbilgin, Kemal
mTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signaling molecules in rat ovaries and uteruses in stages of the estrous cycle. Young adult female rats were divided into four groups as proestrous, estrous, metestrous and diestrous according to vaginal smears. Immunohistochemical staining of mTORC1, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 was performed and pAKT1/2/3 and ERK1 were also analyzed using western blotting on ovarian and uterine tissue samples. According to our results, PI3K/Akt/ mTOR and ERK/pERK showed an increase in the rat ovulation period. When all the groups were evaluated the immunoreactivities for all of the antibodies were detected in the oocytes, granulosa and theca cells, corpus luteum and stroma of ovary and lamina propria, surface and glandular epithelium of uterus with the strongest observed with anti-ERK1 antibody and then with a decreasing trend with anti-mTORC1, anti-pAkt1/2/3, anti-IGF1, anti-PI3K and anti-pERK1/2 antibodies in the proestrus and estrus stages. Differently from other parts of the ovary, highest antibody expression in the corpus luteum was observed in the metestrous stage. Moreover, the existence of pAKT1/2/3 and ERK1 proteins was confirmed with the Western blotting technique. We suggest that mTOR and mTOR-related ERK signaling molecules may participate in the rat ovulation process.
Role of Proopiomelanocortin in Preventing Miscarriage
(Sci Printers & Publ Inc, 2017) Ozbilgin, Kemal; Kahraman, Burcu; Atay, Coskun; Vatansever, Seda; Uluer, Elgin T.; Ozcakir, Tayfun
OBJECTIVE: To compare the distribution of proopiomelanocortin (POMC) in decidua and placenta samples from missed abortion and voluntary termination cases in order to research the effects in the etiology of missed abortion. STUDY DESIGN: Decidual materials were collected from patients who were diagnosed with missed abortion (n=19) and legal voluntary termination cases (n=15) under 10 gestational weeks. Materials were divided into 2 groups for examination. For all samples, POMC primary antibody was performed by immunohistochemical staining. The number of stained cells was calculated by using the H-score technique. RESULTS: In the missed abortion group the mean age was 28.7 (18-41), and in the control group the mean age was 27.5 (21-37). POMC immunoreactivity was determined to be lower in the parenchyma and placenta of the missed abortion group than those of the control group. POMC immunoreactivities were found to be higher in both the syncytiotrophoblast and cytotrophoblast cells of the missed abortion group than those of the control group (p <0.005). CONCLUSION: POMC polypeptide precursor and process. Local production has become a paradigmatic has a role in the partiirition of POMC in placenta and decidua may influence pregnancy and may have a role in missed abortion pathogenesis.