Browsing by Author "Sarigul, Figen"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • Thumbnail Image
    Article
    Citation - WoS: 3
    Citation - Scopus: 4
    Investigation of Compatibility of Severe Acute Respiratory Syndrome Coronavirus 2 Reverse Transcriptase-Pcr Kits Containing Different Gene Targets During Coronavirus Disease 2019 Pandemic
    (Future Medicine Ltd, 2020) Sarigul, Figen; Doluca, Osman; Akhan, Sila; Sayan, Murat
    Aim:In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.Materials & methods:Samples were tested by Bio-Speddy(R)(RdRpgene) and Diagnovital(R)(RdRp + Egenes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.Results:Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.Conclusion:We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.