Browsing by Author "Simsek, Fatma"

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Citation - WoS: 59
Citation - Scopus: 65
Brain Regions Associated With Risk and Resistance for Bipolar I Disorder: a Voxel-Based Mri Study of Patients With Bipolar Disorder and Their Healthy Siblings
(Wiley-Blackwell, 2014) Eker, Cagdas; Simsek, Fatma; Yilmazer, Evrim Ebru; Kitis, Omer; Cinar, Cem; Eker, Ozlem Donat; Coburn, Kerry
ObjectiveBipolar I disorder is a highly heritable disorder but not all siblings manifest with the illness, even though they may share similar genetic and environmental risk factors. Thus, sibling studies may help to identify brain structural endophenotypes associated with risk and resistance for the disorder. MethodsStructural magnetic resonance imaging (MRI) scans were acquired for 28 euthymic patients with bipolar disorder, their healthy siblings, and 30 unrelated healthy controls. Statistical Parametric Mapping 8 (SPM8) was used to identify group differences in regional gray matter volume by voxel-based morphometry (VBM). ResultsUsing analysis of covariance, gray matter analysis of the groups revealed a group effect indicating that the left orbitofrontal cortex [Brodmann area (BA) 11] was smaller in patients with bipolar disorder than in unrelated healthy controls [F=14.83, p<0.05 (family-wise error); 7mm(3)]. Paired t-tests indicated that the orbitofrontal cortex of patients with bipolar disorder [t=5.19, p<0.05 (family-wise error); 37mm(3)] and their healthy siblings [t=3.89, p<0.001 (uncorrected); 63mm(3)] was smaller than in unrelated healthy controls, and that the left dorsolateral prefrontal cortex was larger in healthy siblings than in patients with bipolar disorder [t=4.28, p<0.001 (uncorrected); 323mm(3)] and unrelated healthy controls [t=4.36, p<0.001 (uncorrected); 245mm(3)]. Additional region-of-interest analyses also found volume deficits in the right cerebellum of patients with bipolar disorder [t=3.92, p<0.001 (uncorrected); 178mm(3)] and their healthy siblings [t=4.23, p<0.001 (uncorrected); 489mm(3)], and in the left precentral gyrus of patients with bipolar disorder [t=3.61, p<0.001 (uncorrected); 115mm(3)] compared to unrelated healthy controls. ConclusionsThe results of this study suggest that a reduction in the volume of the orbitofrontal cortex, which plays a role in the automatic regulation of emotions and is a part of the medial prefrontal network, is associated with the heritability of bipolar disorder. Conversely, increased dorsolateral prefrontal cortex volume may be a neural marker of a resistance factor as it is part of a network of voluntary emotion regulation and balances the effects of the disrupted automatic emotion regulation system.
Citation - WoS: 7
An in Vitro Study in Which New Boron Derivatives Maybe an Option for Breast Cancer Treatment
(Kare Publ, 2019) Simsek, Fatma; Inan, Sevinc; Korkmaz, Mehmet
Objectives: We aimed to investigate the distribution of immunoreactivities of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and inducible NOS (iNOS) on breast cancer cells in response to treatment with boron derivatives. Methods: We initially analyzed the cytotoxic effect and IC50 value of boron by MTT assay. For the evaluation of the angiogenesis, expression level of antibodies was detected to following boron derivatives such as boric acid, boron penta (BP), and T-Boron (DPD) in the absence of boron treatment using the indirect immunohistochemical method.The evaluation of these staining was done using the H-scoring system. Results: It was found that immunoreactivities of VEGF, eNOS, and iNOS increased on control compared to those of the cells of MDA-MB231 human breast cancer cell line. Following boron derivatives treatment, it was observed that they were inhibited the VEGF/NOS labeling in MDA-MB-231 breast cancer cells. Conclusion: The present data suggest that BP, especially DPD, inhibits the angiogenesis of breast cancer cells through VEGF pathway. From this point, these boron derivatives may provide a novel therapeutic approach for breast cancer treatment.
Citation - WoS: 9
Citation - Scopus: 8
Molecular Mechanisms Involved in Pre-Eclampsia Through Expressional Regulation of Endothelin-1
(W B Saunders Co Ltd, 2022) Simsek, Fatma; Turunc, Ezgi; Keskin-Arslan, Elif; Erol, Hilal; Acar, Selin; Atakul, Bahar Konuralp; Aydogmus, Serpil
Introduction: Preeclampsia (PE) is a condition affecting 2-8% of all pregnancies and is a leading cause of perinatal morbidity and mortality. In our study; we aim to investigate the differences in endothelin-1 (ET-1) at both tissue and blood level in the placenta, umbilical cord, and maternal blood obtained from different experimental groups and the changes in the contraction response of umbilical arteries in order to explain how PE affects mother and fetus. Methods: Umbilical cord and placenta samples were obtained from normotensive controls (n = 10) and patients with preeclampsia (n = 10), aged 20-39 years, who delivered by cesarean section at term (between 37 and 39 weeks). All samples were investigated with isolated tissue bath, histopathological, immunohistochemical and real-time PCR methods. Results: ET-1 messenger RNA expression levels and immunoreactivity were found significantly higher in the PE group while microRNA-1 and microRNA-125b (miR-125b) levels were significantly decreased in placenta compared to control. miR-125b levels were found significantly higher in maternal and umbilical cord blood samples of the PE group. The enlargement in intervillous space, decrease in villous branching, increase in syncytial knots and smaller lumen areas in umblicard cord vessels were also observed. In tissue bath experiments, there were no significant differences in ET-1 responses between groups. Discussion: We tried to evaluate molecular mechanisms of PE pathogenesis through expressional regulation and contraction response of ET-1. Although quite abundant work in this field has previously highlighted the importance of ET-1 system, further work is needed to determine the molecular mechanisms underlying expressional regulation of ET-1 in PE.