Browsing by Author "Soylu, Mehmet"

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    Covid-19 Antibody Levels Among Various Vaccination Groups, One-Year Antibody Follow-Up in Two University Hospitals From Western and Central Turkey
    (Mdpi, 2024) Soylu, Mehmet; Sağıroğlu, Pınar; Ozarslan, Muhammed Alper; Acet, Oguzhan; Yüce, Zeynep Tuere; İzci Çetinkaya, Feyza; Durmaz, Seyfi; Türkeş, Ayşın Zeytinoğlu
    Various clinical outcomes, reinfections, vaccination programs, and antibody responses resulted from the COVID-19 pandemic. This study investigated the time-dependent changes in SARS-CoV-2 antibody responses in infected and/or vaccinated and unvaccinated individuals and to provide insights into spike and nucleocapsid antibodies, which fluctuate during infectious and non-infectious states. This cohort study was carried out at the Ege University Faculty of Medicine hospital in Izmir (western Turkey) and the Erciyes University Faculty of Medicine hospital in Kayseri (central Turkey) between December 2021 and January 2023, which coincided with the second half of COVID-19 pandemic. The study included 100 COVID-19 PCR-positive patients and 190 healthcare workers (HCWs). Antibody levels were followed up via quantitative anti-SARS-CoV-2 spike and qualitative anti-nucleocapsid immunoassays (Elecsys (TM)). Antibody levels declined after infection but persisted for at least 6-8 months. Individuals who had received only CoronaVac had higher anti-nucleocapsid antibody levels in the early months than those who received mixed vaccination. However, anti-spike antibodies persisted longer and at higher levels in individuals who had received mixed vaccinations. This suggests that combining two different vaccine platforms may provide a synergistic effect, resulting in more durable and broad-spectrum immunity against SARS-CoV-2. The study provides information about the vaccination and antibody status of healthcare workers in the second half of the pandemic and provides valuable insights into the dynamics of antibody responses to COVID-19 infection and vaccination.
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    Evaluation of Alternative Clinical Samples for the Detection of SARS-CoV and Influenza Virus by Automated Multiplex RT-PCR
    (J Infection Developing Countries, 2025) Ozarslan, Muhammed A.; Parkan, Omur M.; Soylu, Mehmet; Acet, Oguzhan; Gokahmetoglu, Selma; Yuce, Zeynep Ture; Erensoy, Selda
    Introduction: The aim of this study was to compare the performance of different clinical specimens-nasopharyngeal (NP) swabs collected by healthcare professionals (HCP-NP), self-collected nasal swabs (Sc-N), and saliva samples (S)-in diagnostic tests for investigating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and influenza A/B RNA. Methodology: These clinical samples were collected from 404 symptomatic cases and tested with the SARS-CoV-2 and influenza A/B RNA tests on the cobas 6800 System of Roche Molecular Systems (Roche Molecular Systems, Pleasanton, USA). The SARS-CoV-2 or influenza virus infection status was determined for all patients based on the predefined criteria and corresponding algorithms. Positive and negative predictive values (PPV, NPV), sensitivity, specificity, coefficient of variation (CV), interrater reliability, correlation, ,and days of sample collection of these three sample types were analyzed. Results: There was almost perfect agreement between the these sample types for the diagnosis of SARS-CoV-2 and influenza A. The overall performance (PPV, NPV, sensitivity) and reproducibility (CV <= 6%) were favorable. Additionally, they showed similar trends for days of sample collection. Conclusions: Diagnostic detection of SARS-CoV-2 and influenza RNA from Sc-N and S samples was comparable to HCP-NP samples. Using these samples would provide an advantage in diagnosing SARS-CoV-2 and influenza A infection, as they can be easily collected without the need for viral transport media.
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    Hepatit C Serolojik Tanısında Anti-HCV Antikoru Düşük Pozitif ve Sınır Değerlerin İki Farklı Enzim İmmunoassay Yöntemiyle Değerlendirilmesi
    (2024) Zeytınoglu, Aysın; Soylu, Mehmet; Uysal, Ayça Aydın
    Amaç: Hepatit C virüsü (HCV) akut ve kronik hepatit tablolarında rol oynayan bir viral etkendir. HCV enfeksiyonunun tanısında en sık başvurulan yöntemler anti-HCV antikorlarının saptanması, alanin transaminaz düzeylerinin (ALT) ölçülmesi ve HCV-RNA pozitifliği değerlendirilmesidir. Bu çalışmada kanda iki farklı enzim immünoassay tabanlı test olan enzim bağlı floresan test (ELFA) ve kemilüminesans immün assay (CLIA) yöntemlerinin en az birinde sınır değer ve zayıf pozitiflik olarak değerlendirilen hastaların HCV antikor indeks değerleri ile olguların ALT ve HCV-RNA sonuçları karşılaştırılmıştır. Yöntem: Ekim 2018-Kasım 2019 tarihleri arasında hastanemize başvuran 59 hastanın serum örneklerinin CLIA sistemindeki sinyal/cut-off (S/CO) ve ELFA sistemindeki Test Value (TV) değerlerine göre zayıf pozitif ve sınır değer sonuçlar çalışmaya dahil edildi. Bulgular: Serum örneklerinden elde edilen HCV antikorları için S/CO ve TV ortalamaları; ELFA testi için 2.78 TV, CLIA testi için ortalama 2.32 S/CO olarak saptandı. Tüm serum örneklerinin ALT düzeyi ortalaması 28.7 U/L olarak saptandı. ELFA testi ile negatif sonuç verilen 17 hastanın (11 Kadın- 6 Erkek, yaş ortalamaları: 42.2) serum örneklerinde CLIA testi ile zayıf pozitif sonuç elde edildi ve ELFA testi ortalaması 0.24 TV, CLIA testi ortalaması 1.74 S/CO olarak saptandı. ELFA testinde sınır değer olguların ortalaması 0.82 TV; aynı grupta CLIA ortalaması 1.97 S/CO olarak saptandı. Sonuç: Bir sistemle elde edilen HCV antikoru sonuçlarının; zayıf pozitif ve sınır değer sonuçların olanak varsa farklı bir sistemle tekrarı ve HCV-RNA testi ile doğrulanması önerilmekle birlikte pozitiflik için baz alınan “1 S/ CO” ve “1 TV” değerlerinin daha yüksek seviyelere çekilmesinin ileri çalışmalarla desteklenmesi gerekmektedir.