Browsing by Author "Yuce, Zeynep"

Now showing 1 - 3 of 3

Citation - WoS: 6
Citation - Scopus: 5
Acrylamide-Encapsulated Glucose Oxidase Inhibits Breast Cancer Cell Viability
(Walter De Gruyter Gmbh, 2020) Rrustemi, Trendelina; Geyik, Oyku Gonul; Ozkaya, Ali Burak; Ozturk, Taylan Kurtulus; Yuce, Zeynep; Kilinc, Ali
Objectives: Cancer cells modulate metabolic pathways to ensure continuity of energy, macromolecules and redoxhomeostasis. Although these vulnerabilities are often targeted individually, targeting all with an enzyme may prove a novel approach. However, therapeutic enzymes are prone to proteolytic degradation and neutralizing antibodies leading to a reduced half-life and effectiveness. We hypothesized that glucose oxidase (GOX) enzyme that catalyzes oxidation of glucose and production of hydrogen peroxide, may hit all these targets by depleting glucose; crippling anabolic pathways and producing reactive oxygen species (ROS); unbalancing redox homeostasis. Methods: We encapsulated GOX in an acrylamide layer and then performed activity assays in denaturizing settings to determine protection provided by encapsulation. Afterwards, we tested the effects of encapsulated (enGOX) and free (fGOX) enzyme on MCF-7 breast cancer cells. Results: GOX preserved 70% of its activity following encapsulation. When fGOX and enGOX treated with guanidinium chloride, fGOX lost approximately 72% of its activity, while enGOX only lost 30%. Both forms demonstrated remarkable resilience against degradation by proteinase K and inhibited viability of MCF-7 cells in an activity-dependent manner. Conclusions: Encapsulation provided protection to GOX against denaturation without reducing its activity, which would prolong half-life of the enzyme when administered intravenously.
Investigation of the Effect of Repeated Acetaminophen Usage on Hippocampus, Prefrontal Cortex and Liver Igf-1 and Mmp-2 Levels in Rats
(Wiley, 2019) Karakilic, Ash; Kizildag, Servet; Yuce, Zeynep; Seval Çelik, Yasemin; Kandis, Sevim; Bozan, Hemdem Rodi; Koc, Bazar; İnan, Sevinç
[Abstract Not Available]
Repeated Acetaminophen Administration Damaged Hippocampal Tissue but Did Not Affect Prefrontal Cortex or Anxiety Behaviors
(Nencki Inst Experimental Biology, 2022) Karakilic, Asli; Kizildag, Servet; Yuce, Zeynep; Seval Çelik, Yasemin; Kandis, Sevim; Bozan, Hemdem Rodi; Koc, Basar
Acetaminophen is one of the most widely used over-the-counter drugs worldwide for the treatment of pain and fever. Although acetaminophen use is known to impair hippocampus-related learning and memory, its effect on anxiety is not clear. Insulin-like growth factor-1 (IGF-1) and matrix metalloproteinase-2 (MMP2) are important for cellular survival, maintenance and tissue integrity. The aim of this study was to investigate the dose-dependent effects of acetaminophen on anxiety levels as well as on hippocampus, prefrontal cortex and liver tissue. Doses of 100, 200 and 400 mg/kg acetaminophen were administered to male Sprague Dawley rats for 11 days and anxiety tests were conducted on the last day. Twenty-four hours after the last acetaminophen administration, all animals were sacrificed and hippocampus, prefrontal cortex and liver tissues were removed for analyses. Hippocampal IGF-1 and MMP2 levels were shown to decrease only at the highest dose of acetaminophen, which was accompanied by pathological changes in histology. The prefrontal cortex was not affected. Behavioral analyses also did not indicate changes in anxiety levels in the rats. Liver IGF-1 and MMP2 levels decreased in all experimental groups. Serum alanine aminotransferase and aspartate aminotransferase levels increased in the 200 mg/kg and 400 mg/kg acetaminophen groups. Our findings showed that varying doses of acetaminophen did not affect the prefrontal cortex or anxiety levels. Further research is needed to elucidate the hippocampal and hepatic protective roles of IGF-1 and MMP2 in acetaminophen toxicity and their potential use in therapeutic approaches.