Please use this identifier to cite or link to this item:
https://hdl.handle.net/20.500.14365/1118
Title: | Effects of Simvastatin on Matrix Metalloproteinase Regulation in Il-1 Beta-Induced Sw1353 Cells | Authors: | Cecen, Berivan Keles, Didem Oktay, Gulgun Kozaci, Leyla Didem |
Keywords: | Chondrosarcoma Simvastatin MMP TIMP Articular Chondrocytes Inflammatory Response Intracerebral Hemorrhage Tissue Inhibitors Autophagy Cartilage Expression Cancer Degradation Mechanisms |
Publisher: | Elsevier Ireland Ltd | Abstract: | The present study shows the basis for the anti-inflammatory effects of statins in interleukin 1 beta (IL-15) induced SW1353 chondrosarcoma cell-line. The cells were pre-treated with simvastatin (5 mu M, 10 mu M, and 50 mu M), followed by IL-1 beta (5 ng/mL) stimulation. Effects of simvastatin on cell viability and cytotoxicity of chondrocytes were measured with WST-1 and lactate dehydrogenase (LDH) assays, respectively. Under inflammatory conditions, in the absence/presence of simvastatin, the changes in matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expression levels were examined. Expression levels of MMP-1, -2, -3, -9, -13, and TIMP-1 and -2 were examined by qPCR. MMP-1, -9, -13, TIMP-1, and -2 levels were also determined by Western blotting. Gelatin zymography was performed to analyze the released and intracellular MMP-2 and MMP-9 activity levels. The results showed that simvastatin downregulated the degradation related genes MMP3, MMP-13, MMP-2, MMP-9 and TIMP-2 in a dose-dependent manner. | URI: | https://doi.org/10.1016/j.cbi.2019.108730 https://hdl.handle.net/20.500.14365/1118 |
ISSN: | 0009-2797 1872-7786 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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