Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14365/2390
Title: Investigation of Compatibility of Severe Acute Respiratory Syndrome Coronavirus 2 Reverse Transcriptase-Pcr Kits Containing Different Gene Targets During Coronavirus Disease 2019 Pandemic
Authors: Sarigul, Figen
Doluca, Osman
Akhan, Sila
Sayan, Murat
Keywords: coronavirus
COVID-19
RT-PCR
SARS-CoV-2
Outbreak
Publisher: Future Medicine Ltd
Abstract: Aim:In the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), reverse transcriptase-PCR (RT-PCR) technique is often used. We evaluated the compatibility of SARS-CoV-2 RT-PCR kits containing different gene targets during the pandemic.Materials & methods:Samples were tested by Bio-Speddy(R)(RdRpgene) and Diagnovital(R)(RdRp + Egenes). The correlation between two assays were determined by Deming regression analysis and chi-square analyses.Results:Diagnovital PCR kit showed amplification in a narrow Ct range and conveniently sharper exponential amplification curves than Bio-Speedy PCR kit. While the correlation between the findings of the two kits was apparent even with single gene target, this correlation increased when a secondary biomarker was added to the correlation calculations.Conclusion:We have observed high correlation between different PCR kits, however, using different PCR kits during the pandemic may provide a more accurate diagnosis of SARS-CoV-2, since despite correlation there are a number of patients showing contradicting diagnosis.
URI: https://doi.org/10.2217/fvl-2020-0169
https://hdl.handle.net/20.500.14365/2390
ISSN: 1746-0794
1746-0808
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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