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https://hdl.handle.net/20.500.14365/2431
Title: | Evaluation of Lateral Flow and Elisa Techniques for Detecting Igg and Igm Antibodies in Covid-19 Cases in Turkiye | Other Titles: | Évaluation du flux latéral et des méthodes ELISA pour la détection des anticorps IgG et IgM parmi les cas de COVID-19 en Türkiye | Authors: | Arikan, Ayse Doluca, Osman Akhan, Sila Sanlidag, Tamer Sayan, Murat |
Keywords: | COVID-19 SARS-CoV-2 lateral flow assay enzyme-linked immunosorbent assay antibody |
Publisher: | Who Eastern Mediterranean Regional Office | Abstract: | Background: Antibody testing can complement molecular assays for detecting COVID-19.Aims: We evaluated the concurrence between lateral flow assay and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).Methods: The study was conducted at Kocaeli University, Turkiye. We used a lateral flow assay and ELISA to test serum samples from COVID-19 cases, confirmed by polymerase chain reaction assays (study group) and pre-pandemic stored serum samples (control group). We used Deming regression to evaluate the antibody measurements.Results: The study group included 100 COVID-19 cases, and the control group included pre-pandemic samples from 156 individuals. The lateral flow assay detected immunoglobulin M (IgM) and G (IgG) antibodies in 35 and 37 study group samples. ELISA detected IgM nucleocapsid (N) antibodies in 18 samples, and IgG (N) and IgG spike 1 (S1) antibodies in 31 and 29 samples, respectively. None of the techniques detected antibodies in the control samples. Strong correlations were found between lateral flow IgG (N+ receptor-binding domain + S1) and ELISA IgG (S) (r = 0.93, P < 0.01) and ELISA IgG (N) (r = 0.81, P < 0.01). Weaker correlations were seen between ELISA IgG S and IgG N (r = 0.79, P < 0.01) and lateral flow assay and ELISA IgM (N) (r = 0.70, P < 0.01).Conclusion: Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult. | URI: | https://doi.org/10.26719/emhj.23.011 https://hdl.handle.net/20.500.14365/2431 |
ISSN: | 1020-3397 1687-1634 |
Appears in Collections: | PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection |
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