Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.14365/4935
Title: Tetracaine Downregulates Matrix Metalloproteinase Activity and Inhibits Invasiveness of Strongly Metastatic Mda-Mb Human Breast Cancer Cells
Authors: Keleş Bartık, Didem
Sipahi, M.
İnanç-Sürer
Djamgoz, M.B.
Oktay, G.
Keywords: Breast cancer
Invasion
Matrix metalloproteinases
Tetracaine
Voltage-gated sodium channels
Publisher: Elsevier Ireland Ltd
Abstract: Tetracaine, a long-acting amino ester-type local anesthetic, prevents the initiation and propagation of action potentials by reversibly blocking voltage-gated sodium channels (VGSCs). These channels, which are highly expressed in several carcinomas (e.g. breast, prostate, colon and lung cancers) have been implicated in promoting metastatic behaviours. Recent evidence suggests that local anesthetics can suppress cancer progression. In this paper, we aimed to explore whether tetracaine would reduce the invasive characteristics of breast cancer cells. In a comparative approach, we used two cell lines of contracting metastatic potential: MDA-MB-231 (strongly metastatic) and MCF-7 (weakly metastatic). Tetracaine (50 μM and 75 μM) did not affect the proliferation of both MDA-MB-231 and MCF-7 cells. Importantly, tetracaine suppressed the migratory, invasive, and adhesive capacities of MDA-MB-231 cells; there was no effect on the motility of MCF-7 cells. Tetracaine treatment also significantly decreased the expression and activity levels of MMP-2 and MMP-9, whilst increasing TIMP-2 expression in MDA-MB-231 cells. On the other hand, VGSC α/Nav1.5 and VGSC-β1 mRNA and protein expression levels were not affected. We conclude that tetracaine has anti-invasive effects on breast cancer cells and may be exploited clinically, for example, in surgery and/or in combination therapies. © 2023 Elsevier B.V.
URI: https://doi.org/10.1016/j.cbi.2023.110730
https://hdl.handle.net/20.500.14365/4935
ISSN: 0009-2797
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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