Browsing by Author "Elibol, Murat"

Now showing 1 - 7 of 7

Citation - WoS: 1
Citation - Scopus: 5
Biogenic Nanoparticle Synthesis Using Marine Alga Schizochytrium Sp.
(Springer, 2020) Mutaf, Tuğçe; Çalışkan, Gülizar; Meydan, Cafer; Oncel, Suphi Surisvan; Elibol, Murat
Nanotechnology is one of the most promising science and technology discipline that targets to bring new solutions for many applications in biotechnology, biomedical, energy and cosmetic industry by improving particles and devices scale of nanometers. Various sized and shaped nanoparticles can be synthesized by several methods. Up to now, scientists prefer physical and chemical fabrication of nanoparticles. But, these methods contain use of toxic, expensive and non-environmentalist solvents, reducing and stabilising agents. For a sustainable science, there is a necessity development of more eco-friendly, cost-effective and trustable alternative processes. In this context, using biological sources as reaction agent, have a strong potential. Plants, bacteria, fungi are essential biological sources for transformation of metals to nanoparticles. Many researchers focus on fungi and bacteriological potential in nanofabrication whereas algae are highly intriguing biological systems in nanotechnological approach. Some of cyanobacteria and algae have previously been used to synthesize intracellular or extracellular metal nanoparticles. Most of the research concentrate especially on gold and silver nanoparticle production from algae. In this work; bioreduction of silver, zinc and iron metals have been investigated using culture supernatant of marine algae Schizochytrium sp. For characterization of nanoparticles, UV visible spectroscopy, zeta sizer were used. Nanoparticle size was determined by zeta sizer and particles' surface plasmon resonance band detected by UV-Visible Spectroscopy.
Citation - WoS: 25
Citation - Scopus: 28
Green Synthesis and Characterization of Titanium Nanoparticles Using Microalga, Phaeodactylum Tricornutum
(Taylor & Francis Inc, 2022) Çalışkan, Gülizar; Mutaf, Tuğçe; Agba, Hasan Cenk; Elibol, Murat
Nanoparticles synthesized from microalgae offer a newly discovered process that is open to improvement. In this study supernatant of Phaeodactylum tricornutum (P. tricornutum) culture was used for this purpose. Firstly, the effects of some variables, namely titanium concentrations, titanium:supernatant ratio, pH, incubation time, and mixing speed on nanoparticle synthesis were investigated by using statistical design method in shaken culture as well as bioreactor. The average nanoparticle size synthesized in optimum conditions which were pH 7.5 with 300 rpm for 1 h was found as 50 nm. In the second part, nanoparticles were coated with a chitosan solution to protect their stability and increase their potential. Therefore, the antimicrobial activity showed a reasonable effect on these nanoparticles coated with chitosan. Nanoparticles produced had interestingly shown 99% antistatic properties. According to the cytotoxicity test, these nanoparticles showed a high cytotoxic effect on different cancer cell lines. The results obtained in the present study can be considered promising outcomes for possible future antimicrobial, biogenic and antistatic studies particularly in biomedical applications.
Citation - WoS: 13
Citation - Scopus: 20
Green Synthesis of Metal Nanoparticles Using Microalga Galdieria Sp.
(Springer, 2020) Çalışkan, Gülizar; Mutaf, Tuğçe; Oncel, Suphi Surisvan; Elibol, Murat
Green synthesis of nanoparticles has recently been a preferred method since it offers useful approaches such as non-toxic, biocompatible, environmentally friendly, cost-effective, stable product and trustable alternative processes compared to other methods. Various biological sources such as plant, algae, fungus and bacteria are widely used in biological synthesis of nanoparticles. Algae are more adapted organisms compared to the others for having high growth rate and biomass productivity, high heavy metal accumulation capacity, etc. Nanoparticles can be used in various area such as antimicrobial, antifungal, antioxidant agent, admixture of biosensor and drug transport/release, also diagnosis and treatment systems. Since each organism has different biochemical composition and metabolic pathways, their synthesized nanoparticles are going to show various characteristics and application area. In this study, silver, iron (II) and zinc nanoparticles were produced by using microalga Galdieria sp. The synthesized nanoparticles were characterized by using UV visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and zeta sizer. Antimicrobial activity against gram negative and gram-positive bacteria was also examined throughout the study. In conclusion, the potential of this biogenic nanoparticle was discussed.
Citation - WoS: 4
Citation - Scopus: 7
Green Synthesis of Titanium Nanoparticles Using a Sustainable Microalgal Metabolite Solution for Potential Biotechnological Activities
(Wiley, 2023) Mutaf, Tugce; Çalışkan, Gülizar; Özel, Hulki; Akağaç, Gülşah; Öncel, Suphi S.; Elibol, Murat
In this study, green synthesis of titanium nanoparticles using liquids metabolites of microalgae, Porphyridium cruentum, was performed to evaluate potential biotechnological activity. The rising rates of multidrug-resistant bacteria and the number of cancer patients are driving the search for novel antimicrobial and anticancer agents to combat this threat. In recent years, with the increasing number of studies, nanomaterials are starting to be better understood and are emerging as a solution to this problem. Especially, green synthesized nanoparticles with anticancer, antioxidant, and antimicrobial activities have potential in biomedical applications because of their eco-friendly and biocompatible nature. Scanning electron microscopy (SEM) images revealed that spherical shaped Ti-NPs' size ranged from 62 to 133 nm. This study aimed to assess the effectiveness of antibacterial activity of Ti-NPs and chitosan-coated Ti-NPs against Escherichia coli and Staphylococcus aureus using disc diffusion assay. It demonstrated the concentration-dependent cytotoxic effect of Ti-NPs of human prostate adenocarcinoma (PC-3), human alveolar adenocarcinoma (A549), and human mammary gland adenocarcinoma (MDA-MB) cancer cell lines. This present study shows promising outcomes for possible future applications of synthesized Ti-NPs as a novel antibacterial and cytotoxic agent for biomedical applications such as drug delivery, biosensor, and hyperthermia.
Citation - WoS: 3
Metal Nanopartiküllerin Mikroalgler Aracılığı ile Yeşil Sentezi
(2023) Mutaf, Tuğçe; Çalışkan Bilgin, Gülizar; Öncel, Suphi; Elibol, Murat
Yeşil sentez olarak adlandırılan, nanopartiküllerin biyolojik kaynaklar aracılığı ile sentezlenmesine olan ilgi son yıllarda artış göstermiştir. Bunun temel nedeni geleneksel yöntemler olan fiziksel ve kimyasal yöntemlerde indirgeyici ve stabilize edici ajanlar olarak yüksek miktarlarda toksik kimyasala ihtiyaç duyuluyor olmasıdır. Daha çevre dostu ve insan sağlığı için tehdit oluşturmayan bitki, fungus, bakteri, alg gibi organizmalar yeşil nanopartikül sentezi için alternatif kaynaklardır. Sucul mikroorganizmalar olan mikroalgler üretmiş oldukları proteinler, vitaminler, pigmentler, yağ asitleri, hücre içi- hücre dışı polisakkaritler gibi fonksiyonel özelliğe sahip metabolitler sayesinde uzun yıllardır gıda, kozmetik ve ilaç endüstrilerinde formülasyonlara eklenmektedir. Bunların yanı sıra, son yıllarda yapılan çalışmalarla nanopartikül sentezinde de yüksek potansiyele sahip oldukları görülmüştür. Özellikle metal iyonlarının depolanmasını ve detoksifikasyonunu yapabildiklerinden ve metal iyonlarını elementel hale indirgeyen hücre içi ve hücre dışı metabolitlerce zengin olduklarından, metal nanopartiküllerin sentezi için yüksek potansiyele sahiptirler. Son yıllarda mikroalglerden nanopartikül sentezine odaklanmış olan yayın sayısı artmış ve pek çok mikroalg türünün gümüş, altın, titanyum, çinko, demir vb. metal nanopartikülleri hücre içi ve hücre dışı yollarla sentezleme potansiyeli araştırılmıştır. Bu derleme makale kapsamında, nanopartikül sentezi için çalışılmış olan mikroalg ve siyanobakteri türleri, kullanılan farklı sentez yöntemleri, nanopartiküllerin sentez mekanizması, temel karakterizasyon yöntemleri ve yeşil sentezle üretilen nanopartiküllerin antimikrobiyal aktivitelerine odaklanılmıştır
Nanopartikül Biyosentezinde Ortalama Çap Tahmini ve Optimizasyonu için Yanıt Yüzeyi Yöntemi ve Makine Öğreniminin Birleştirilmesi
(IEEE, 2024) Çalışkan Bilgin, Gülizar; Topalli, Ayca Kumluca; Kilic, Tugce Mutaf; Elibol, Murat
The synthesis of nanoparticles from biological sources by green synthesis method and production optimization studies are increasing in popularity today. However, the variability of biological source and environmental effects in such processes leads to different morphology and functionality in the final product. In this study, microalgae was used as a bioreduction agent in nanoparticle synthesis and analyses of the harmonic mean particle diameter of FeSO4 concentration and its ratio with microalgae medium were carried out in particle synthesis. In this two-stage study, the experimental design was carried out first, and the particle diameters obtained by data generation were developed by machine learning. The error rates at both stages were compared and improvements were recorded. As a result, a new low-cost, fast, simple and environmentally friendly approach was introduced to solve the data insufficiency problem and used in particle diameter estimation. The results obtained showed that the proposed combined strategy provides better nanoparticle size estimates than the statistical approach alone. The proposed method is applicable to a wide range of biotechnology and bioengineering applications with significant advanced knowledge.
Porphyridium Cruentum as a Biological Component for the Green Synthesis of Metal Nanoparticles and for the Evaluation of Their Antimicrobial Activity
(Field Crops Central Research Institute, 2025) Mutaf, Tugce; Çalışkan, Gülizar; Oncel, Suphi; Elibol, Murat
Silver nanoparticles are an alternative to new-generation antimicrobial agents with their antimicrobial activity. Iron and zinc nanoparticles can potentially be used as UV protection in various applications. Nowadays, green synthesis of nanoparticles as a sustainable alternative attracts attention. Microalgae are promising in nanoparticle synthesis among biological sources due to their high biomass productivity and heavy metal accumulation ability. The present study aimed to investigate the potential of synthesizing intracellular silver, zinc and iron nanoparticles from Porphyridium cruentum microalgae. For nanoparticle synthesis, the effects of metal solution concentration and amount of biomass on particle size were investigated. The nanoparticles were characterized by dynamic light scattering, UV-vis spectrophotometry, and antimicrobial activity test. Silver nanoparticles of 169.7 nm, zinc nanoparticles of 189 nm, and iron nanoparticles of 356.7 nm were characterized by DLS. 169.7 nm silver nanoparticles were synthesized with 9.83 mM AgNO3 concentration and 0.19 mg/ml biomass: metal solution mixing ratio. The surface plasmon resonance band of silver nanoparticles was observed in the 300-350 nm wavelength range. According to the antibacterial activity results of silver nanoparticles, inhibition zone diameters were obtained as 10.83±0.76 mm and 11.33±0.57 mm against Escherichia coli and Staphylococcus aureus bacteria, respectively.