TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14365/4
Browse
5 results
Search Results
Article Histomorphometric Analysis of the Effects of Grape Seed Extract (vitis Vinifera) and Low-Level Laser Therapy (lllt) on Fracture Healing(2020) Kara, Muhammed İsa; İnan, Sevinç; Aydemir, Işıl; Taysı, Seyithan; Aras, Mutan Hamdi; Altundağ Kahraman, Sevil; Ege, Bilal; Kahraman, Sevil Altundag; Kara, İsa; Akgül, HasanAim: In this study, we aim to investigate the effects of supplementary Grape Seed Extract (GSE) and Low-Level Laser Therapy (LLLT)on fracture healing, oxidant and anti-oxidant system in experimental mandible fractures.Materials and Methods: 48 Wistar Albino rats (adult male, n=48) were used in our study. For all the subjects, a vertical fracture linethrough molar teeth in right mandibles was created and internally fixed using a four-hole microplate and four micro screws. Firstly,these subjects were randomly divided into 4 main groups (Control, GSE, LLLT, GSE+LLLLT) of 12 animals each, and then these wereindividually separated into two sub-groups of 7th and 21st days. The number of groups (n=6) was 8 in total. GSE of 300 mg/kg/day were provided to the subjects before they were sacrificed. LLLT of 23 J/cm2 was administered to two different points alongwith the fracture line at intervals of 48 hours for 7 days in the 7th day sub-groups and for 14 days in the 21st day sub-groups. Afterthe procedure, while biochemical values such as TAS, TOS and OSI are measured; histopathologically it was examined in termsof capillary number and width, inflammatory cell, fibroblast count, collagen fibers, osteoblast count, ossification and mature boneformations.Results: For biochemical analyses, there was statistically significant difference only in TAS values on either the 7th or 21st day forthe groups. Histological analyses showed that mandibular fracture healing were significantly better in the GSE and GSE+LLLT groupscompared to the control group. The group of only LLLT had limited recovery while the combination of GSE+LLLT was the best forossification.Conclusions: It is concluded that GSE may be one of the potential methods to accelerate fracture healings in mandibular fracturescommon in oral and maxillofacial surgery clinic and thus help patients recover in shorter time, and however LLLT can have positiveeffects on the process of ossification and recovery only when combined with the extract, which may result in better outcomes inclinical use.Other Evaluation of Jak/Stat Signaling Pathway-Associated Protein Expression at Implantation Period: an Immunohistochemical Study in Rats(2023-10-20) Gürel, Çevik; Kuşçu, Gökçe Ceren; Vatansever, H. Seda; Köse, Can; İnan, Sevinç; Özbilgin, Kemal; Kırmaz, Cengiz; Vatansever, SedaObjective: The implantation period of gestation is a complex process in which numerous molecular pathways play a role. The Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway is one of the evolutionarily conserved cascades used to transduce a multitude signals for several biological events such as implantation and uterine receptivity. Previous studies have indicated that the implantation process is disrupted by the lack of proteins involved in this pathway. However, our literature knowledge showed that there is no study evaluated the expression of JAK/STAT signaling pathwayassociated proteins during the implantation period. This study investigated the expression patterns of JAK/STAT signaling pathway-associated proteins in rats by immunohistochemical (IHC) staining according to gestational days. Methods: In this study, thirty Wistar Albino rats weighing 250-300 g, without any problems in their menstrual cycles, were used. The pregnant animals were sacrificed on the 4th, 5th, and 6th days and histochemical and IHC analysis were performed on the uterine tissues taken from these animals. Results: In this study, protein expression of JAK1, JAK2, JAK3, STAT2, STAT3, STAT4, STAT5, and STAT6 belonging to the JAK/STAT pathway was evaluated in the uterine surface epithelium, gland structures, antimesometrial region, cells of the immune system, myometrium, mesometrial region and decidual cells, which are associated with the implantation process. The result of this evaluation reveals that the expression levels of these proteins in the JAK/STAT pathway vary in different days of the implantation period in implantation-related structures. Conclusion: This study indicates that JAK/STAT signaling pathway-associated proteins can function actively in the regulation of the immunological response of the uterus and embryo-uterus interaction during the implantation period in rats. However, the findings obtained from advanced research on JAK/STAT pathway can be used for treating recurrent pregnancy failures and in enhancing assisted reproductive technology.Article The Effects of Ovarian Vitrification on Mitochondrial Fusion (mfn-1, Mfn 2 and Opa-1), Fission (dnm-1), Mitophagy (parkin, Pink-1) Andtransport (miro-1, Milton) Proteins(2020-12-31) Vatansever, H. Seda; Özbilgin, Kemal; Öztatlıcı, Mustafa; Temel, Merve; İnan, Sevinç; Vatansever, Seda; Özden, Merve TemelObjective: Ovarian cryopreservation is a useful alternative for fertility preservation in assisted reproductivetechnologies. In spite of many advances in the vitrification procedure, this technique is still considered experimental. Therefore in this study, we aimed to investigate the expressions of mitochondrial fusion (MFN1, MFN2 and OPA1), fission (DRP1), mitophagy (PARKIN, PINK1) and transport (MIRO-1, MILTON) proteins in ovarian tissues by qPCR technique after vitrification.Materials and Methods: To investigate the mitochondrial dynamics after vitrification, the ovaries were recoveredfrom 6-8 week old healthy female mice (No: 12) and were divided into vitrification and control groups. Vitrification carried out using ethylene glycol, dimethylsulfoxide and sucrose. After total RNA isolation from ovaries in control and vitrification groups, qPCR technique was performed to determine the expression rate of target genes. The relative gene expressions of the target genes were evaluated according to 2???Ct method.Results: Histological evaluation revealed that ovaries in the control group were shown normal morphology while the tissue integrity of the ovaries in the vitrification group is disrupted, some follicles are degenerated and granulosa cells were shed into antrum. According to our qPCR results, outer membran fusion proteins MFN1 gene expression decreased 1.12 fold and inner membran protein OPA-1 increased 1,36 fold in the vitrification group compared the control group. The mitochondrial fission protein DRP-1 gene expression increased 1.20 fold in the vitrification group. The mitophagy proteins PINK-1 and PARKIN genes expressions decreased 1.34 and 3.75 fold respectively in the vitrification group. The transport proteins; MIRO-1 gene expression decreased 1.16 fold but MILTON (TRAK-1) gene expression sharply increased 2,28 fold compared the control group.Conclusion: The alternation of the mitochondrial dynamics related gene expressions may lead a decrease in themitochondrial function during the ovarian vitrification and may reduce the potential of oocyte maturation and embryo development.Article Meme Kanseri Hücre Hatlarında Propranolol ve Paklitakselin Anjiyogenez Üzerine Etkisi(2019-03-31) Vatansever, H. Seda; Tuğlu, İbrahim; Özbilgin, Kemal; İnan, Sevinç; Şimşek, Fatma; Müftüoğlu, Sevda; Tuğlu, Mehmet İbrahim; Vatansever, SedaAmaç: Bu çalışmanın amacı infantil hemanjiyomvakalarında kullanılan propranolol (PR) ile kemoterapötikbir ajan olarak yaygın kullanılan Paklitakselin (PX) kanserhücreleri üzerine etkisini incelenmesidir.Gereç ve Yöntem: Tripan mavisi ile hücre sayımıyapılarak hücrelerin ikilenme zamanları belirlendi. MTTtesti ile de ilaçların sitotoksik etkisi ve IC50 değerleri analizedildi. İnvazyon yönünden farklı iki meme kanseri hücrehattında (MDA-MB-231 ve MCF-7) anti-VEGF, antieNOS, anti-iNOS ve anti-ERK1/2 primer antikorlarıindirek immunohistokimyasal yöntemle incelendi.İmmunoreaktivitenin değerlendirilmesi için H skorlamasistemi kullanıldı.Bulgular: MTT testi ile hücrelere uygulanacak ilaçdozlarının IC50 değerleri MDA için; PX: 5 nmol, PR: 50µm ve MCF- 7 için; PX: 3,7 nmol, PR: 50 µm olarakbulundu. İmmunohistokimyasal uygulamada kanserhücrelerinde kontrol gruplarının immunoreaktivitesişiddetli ve/veya çok şiddetli artmış iken PX, PR vekombine uygulanan ilaç gruplarında boyanma şiddetianlamlı veya çok anlamlı olarak azaldı.Sonuç: Bu çalışma ile kemoterapötik olarak uygulananpaklitaksele ek olarak anti anjiyogenik ilaç uygulamalarınındamarlarda vazodilatasyon, hücre çoğalması, göçü veyaşam süresini etkilemesi sonucunda anjiyogeneziazaltması veya önlemesi açısından meme kanserinintedavisinde önemli olduğu düşünülmüştür.Article Multidisciplinary Case-Based Small Group Discussions To Integrate Basic Medical Sciences With Clinical Situations(Walter De Gruyter Gmbh, 2020-09-18) Şemin, Makbule İlgi; Ersil Soysal, Dilek; Seval Çelik, Yasemin; Hayran, Murvet; Demir, Ayse Banu; Ozkaya, Ali Burak; İnan, Sevinç; Akdoğan, Gül; Semin, Ilgi; Soysal, Dilek Ersil; Seval-Celik, Yasemin; Güner, Gül AkdoganObjectives Integration of the basic medical sciences with clinical medicine motivates medical students by showing how the fundamental concepts they have learned will come into their future practice. In this context, we created clinical integration sessions (CIS) in our first-year medical curriculum. Methods The instructors of different disciplines wrote the clinical scenarios together. The scenarios were discussed in five sessions with 39 first-year students. The first session's scenario consisted of four brief anemia cases. The next four sessions included a single case, according to the feedback of the students. Students formed groups of 7-8 participants. In the first 2 h, the scenarios were discussed in the groups and questions were answered by the students. In the third hour, the instructors answered the questions together with the students. After the first CIS, written feedback obtained from the students via a survey. Results The survey provided positive feedback on the benefits of active learning within small group discussions, and most of the students thought that their background was sufficient to solve the cases, with some literature search. Conclusion The scenarios, which provide multidisciplinary integration of basic medical sciences and clinical medicine, can be useful educational materials.