TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14365/4
Browse
9 results
Search Results
Article Investigation of Neuraminidase 1 Gene Association in Henoch-Schönlein Purpura (hsp) With Renal Involvement(Pamukkale University, 2022-07-01) Yılmaz, N.B.; Ertan, P.; Yüksel, S.; Neşe, N.; Dinç Horasan, Gönül; Berdeli, A.H.; Horasan, Gönül Dinç; Bahçeci, Nezihe BilgePurpose: HSP is a common small vessel vasculitis. It is the most common cause of non-thrombocytopenic purpura in childhood. The role of genes in etiopathogenesis of the disease, which has not yet been clearly elucidated, is being emphasized. Many genes called sialidases are being studied and is thought that the NEU1 gene may be particularly important in the etiopathogenesis of HSP. The aim of this study is to investigate the role of the NEU1 gene in the etiopathogenesis of HSP and its relation to renal involvement. Materials and methods: Fifty patients followed in the Celal Bayar University Hafsa Sultan Hospital Pediatric Nephrology Department, with the diagnosis of HSP renal involvement were included into the study. For the control group, age and gender matched 50 cases were accepted among the outpatients admitted to Pediatric Department without any chronic diseases. NEU1 gene mutation analysis was performed in blood samples of both patient and control groups by using the Sanger DNA sequencing method. Results: NEU1 genetic mutation was not detected in any HSP patient with renal involvement and control group. Conclusion: In our study, the NEU 1 gene was not found to be associated with HSP nephritis. No changes were detected in the investigated regions of the NEU1 gene. © 2022, Pamukkale University. All rights reserved.Article Absence of Costal Element of the Foramen Transversarium of Atlas Vertebrae: a Case Report(2018) Hayran, Mürvet; Yonguç, Gökşin Nilüfer; Baylan, Hüseyin; Yonguc, NiluferWe detected an atlas vertebra of which costal elements of its foramen transversarium were not developed bilaterally. The costal elementis an arch of bone that form the anterior portion of foramen transversarium. The vertebral arteries running towards cranial cavity aresurrounded and protected by foramina transversarium of upper six cervical vertebrae. Surgeons should be aware of this variation beforeoperations to neck region, because the vertebral arteries may easily be injured due to lack of anterior bony protection of the costalelements. As well as the vertebral artery, the nerve plexus around vertebral artery may also be responsible of some complications due tofailure of this bony protection.Article Covıd-19 Tedavisinde Kullanılan İlaçların Güvenliliği(2021) Gümüştekin, Mukaddes; Barış, Elif; Arici, M. AylinCOVID 19 hastalığı etkeni SARS CoV 2, ateş, öksürük, nefes darlığı gibi semptomlara neden olan bir virüstür. Ülkemizi ve tüm dünyayı etkileyen COVID 19 pandemisinde, spesifik olarak COVID 19 enfeksiyonu tedavisi için geliştirilmemiş ve farklı endikasyonlarda kullanılan ilaçların yeniden konumlandırıldığı ve tedavi ile ilgili pek çok klinik araştırmanın yürütüldüğü bilinmektedir. Hastalığın tedavisinde halen klinik araştırmalarla etkililiği ve güvenliliği tanımlanmış bir ilaç bulunmamaktadır. Tedavi yönetimi, elde edilen klinik deneyime göre güncellenmekte ve farklı ülkelerde farklı tedaviler kullanılmaktadır. Şu an için hastalığın tedavisinde kullanılan ilaçlar arasında; antiviraller, antimalaryaller, antibiyotikler, immunomodülatör ilaçlar ve antikoagulan ilaçlar ön plandadır. Tüm bu bahsedilen ilaç gruplarında yer alan ilaçların, COVID 19 hastalığında kullanımında güvenlilikleri ile ilgili olarak da bilgiler gün geçtikçe artmaktadır. Antivirallerden remdesivire bağlı karaciğer fonksiyon testlerinde yükseklik, lopinavir ritonavire bağlı hiperlipidemi, antimalaryallerden hidroksiklorokin ve antibiyotiklerden azitromisine bağlı QT uzaması, immunomodülatörlerden tosilizumaba bağlı nötropeni, antikoagulanlara bağlı ise kanama riski dikkati çekmektedir. Bu derlemede, COVID 19 hastalığı tedavisinde kullanılan ilaçlara bağlı advers reaksiyonlar literatür ışığında sunulmaktadır.Article Histomorphometric Analysis of the Effects of Grape Seed Extract (vitis Vinifera) and Low-Level Laser Therapy (lllt) on Fracture Healing(2020) Kara, Muhammed İsa; İnan, Sevinç; Aydemir, Işıl; Taysı, Seyithan; Aras, Mutan Hamdi; Altundağ Kahraman, Sevil; Ege, Bilal; Kahraman, Sevil Altundag; Kara, İsa; Akgül, HasanAim: In this study, we aim to investigate the effects of supplementary Grape Seed Extract (GSE) and Low-Level Laser Therapy (LLLT)on fracture healing, oxidant and anti-oxidant system in experimental mandible fractures.Materials and Methods: 48 Wistar Albino rats (adult male, n=48) were used in our study. For all the subjects, a vertical fracture linethrough molar teeth in right mandibles was created and internally fixed using a four-hole microplate and four micro screws. Firstly,these subjects were randomly divided into 4 main groups (Control, GSE, LLLT, GSE+LLLLT) of 12 animals each, and then these wereindividually separated into two sub-groups of 7th and 21st days. The number of groups (n=6) was 8 in total. GSE of 300 mg/kg/day were provided to the subjects before they were sacrificed. LLLT of 23 J/cm2 was administered to two different points alongwith the fracture line at intervals of 48 hours for 7 days in the 7th day sub-groups and for 14 days in the 21st day sub-groups. Afterthe procedure, while biochemical values such as TAS, TOS and OSI are measured; histopathologically it was examined in termsof capillary number and width, inflammatory cell, fibroblast count, collagen fibers, osteoblast count, ossification and mature boneformations.Results: For biochemical analyses, there was statistically significant difference only in TAS values on either the 7th or 21st day forthe groups. Histological analyses showed that mandibular fracture healing were significantly better in the GSE and GSE+LLLT groupscompared to the control group. The group of only LLLT had limited recovery while the combination of GSE+LLLT was the best forossification.Conclusions: It is concluded that GSE may be one of the potential methods to accelerate fracture healings in mandibular fracturescommon in oral and maxillofacial surgery clinic and thus help patients recover in shorter time, and however LLLT can have positiveeffects on the process of ossification and recovery only when combined with the extract, which may result in better outcomes inclinical use.Article Citation - WoS: 1Citation - Scopus: 1Healthcare-Related Hcv Genotype 4d Infections in Kayseri, Turkey(Aves, 2022-11-15) Gokahmetoglu, Selma; Polat, Ceylan; Atalay, Mustafa Altay; Sezgin, Gulten Can; Ergor, Gul; Aygen, Bilgehan; Gursoy, Sebnem; Abacıoğlu, Yusuf Hakan; Gökahmetolu, Selma; Abacolu, Yusuf Hakan; Abacioglu, HakanBackground: The frequency of genotype 4 hepatitis C virus infection is significantly higher in a city compared to other provinces in Turkey. In this study, we aimed to investigate the epidemiology and risk factors of hepatitis C virus genotype 4 infection in Kayseri province of Turkey. Methods: A case-control study was conducted with 61 hepatitis C virus genotype 4-infected patients and 71 controls. A questionnaire was administered to the patients and controls, asking for information about the risk factors of hepatitis C virus transmission. Core/E1 and NS5B regions of hepatitis C virus genome were amplified and sequenced by Sanger method. Phylogenetic analysis and molecular clock analysis were performed. The risk was determined by calculating the odds ratio and 95% CI. Logistic regression analysis was performed to determine the effect of risk factors by controlling for confounding variables. Results: Kayseri isolates were closely related to type 4d sequences but formed a separate cluster. According to the molecular clock analysis, hepatitis C virus genotype 4d entered Kayseri province probably between 1941 and 1988. Blood transfusion and surgical intervention were found to be significant risk factors for the infection. Conclusion: Epidemiological data showed that hepatitis C virus genotype 4d infections are significantly associated with unsafe medical procedures.Article Citation - WoS: 15Citation - Scopus: 14Changes on Hepatitis C Virus Genotype Distribution in Western Turkey: Evaluation of Twelve-Year Data(Aves, 2020-02-15) Duran, Alev Cetin; Cetinkaya, Ozgul Kaya; Sayiner, Ayca Arzu; Seydaoglu, Gulsah; Ozkaratas, Emre; Abacioglu, HakanBackground/Aims: Hepatitis C virus (HCV) prevalence is 1% in Turkey with genotype 1 being the predominant type traditionally. However unique geographical location of Turkey and increasing human migration in the region influences the epidemiology of the infection. The aim of this study was to determine the changes in distribution of HCV genotypes and risk factors. Materials and Methods: In this retrospective single-center study, HCV genotyping results of 558 patients were evaluated in between 2005 and 2016.Three different HCV genotyping assays were used during the 12-year study period;restriction fragment length polymorphism (RFLP), Abbott Real Time HCV Genotype II and Bosphore HCV genotyping kit. Results: The most prevalent HCV genotype was genotype 1 detected in 88.4% of the patients followed by genotype 3 (5.2%),genotype 4 (2.9%),genotype 2 (2.1%), mixed genotypes (1.1%) and genotype 5 (0.3%). Genotype 1a showed an increasing prevalence. There were 19 patients (3.4%) either of foreign nationalities or Turkish citizens living abroad. Genotype 3 was the most common type among these patients which 10.3% had intravenous drug use history. Syrian migrant population differed in terms of HCV genotypes. Genotype 5 detected in two Syrian patients, which is the first report of HCV type 5 in Western Turkey. Among the HCV genotype 4 infected patients, 31.3% were Syrians. Conclusion: Our study showed that although genotype 1b dominance continues, the distribution and prevalence of HCV genotypes are changing in our region mainly due to migration and increase in the frequency of patients with non-traditional risk factors such as intravenous drug use. Monitoring the epidemiology of HCV genotypes may provide guidance in treatment decisions.Article Citation - WoS: 3Citation - Scopus: 3A Minimally Invasive Transfer Method of Mesenchymal Stem Cells To the Intact Periodontal Ligament of Rat Teeth: a Preliminary Study(Tubitak Scientific & Technical Research Council Turkey, 2018-10-25) Gul Amuk, Nisa; Kurt, Gokmen; Kartal Yandim, Melis; Adan, Aysun; Baran, Yusuf; Amuk, Nisa Gul; Yandım, Melis KartalThe aim of this study was to introduce a minimally invasive procedure for mesenchymal stem cell (MSC) transfer into the intact periodontal ligament (PDL) of the molar teeth in rats. Ten 12-week-old Wistar albino rats were used for this preliminary study. MSCs were obtained from bones of two animals and were labeled with green fluorescent protein (GFP). Four animals were randomly selected for MSC injection, while 4 animals served as a control group. Samples were prepared for histological analysis, Cox-2 mRNA expression polymerase chain reaction analysis, and fluorescent microscopy evaluation. The number of total cells, number of osteoclastic cells, and Cox-2 mRNA expression levels of the periodontal tissue of teeth were calculated. The number of total cells was increased with MSC injections in PDL significantly (P < 0.001). The number of osteoclastic cells and Cox-2 mRNA expression were found to be similar for the two groups. GFP-labeled MSCs were observed with an expected luminescence on the smear samples of the PDL with transferred MSCs. The results of this preliminary study demonstrate successful evidence of transferring MSCs to intact FIX in a nonsurgical way and offer a minimally invasive procedure for transfer of MSCs to periodontal tissues.Article Possible Therapeutic Role of Cholinergic Agonists on Covid-19 Related Inflammatory Response(Dokuz Eylul Univ Inst Health Sciences, 2021-02-26) Baris, Elif; Arici, M. AylinSevere acute respiratory syndrome-corona virus-2 (SARS-CoV-2) or coronavirus infectious disease (COVID-19) outbreak is continued to spread all over the world recently with the high mortality and morbidity rates. It is also known well COVID-19 is leading causes of acute lung injury and acute respiratory distress syndrome (ARDS), sepsis and multiorgan failure. Current treatment of COVID-19, includes different strategies targeting preventing viral replication or treating secondary infections and decreasing exaggerated immune response. Although antiviral, antimicrobial, immunomodulatory agents including anti-cytokines and glucocorticoids have been currently applied, there is lack of a specific treatment for COVID-19. In this review, possible therapeutic roles of cholinomimetic drugs in the control of COVID-19 related inflammation is discussed.Article Citation - WoS: 1Determination of a Sample-To Ratio To Predict True-Positivity in Blood Donor Samples Screened for Syphilis by a Chemiluminescent Immunoassay(Aves Press Ltd, 2018) Akcakanat, I. Ebru; Ozbek, Ozgen Alpay; Dogan, Yavuz; Abacioglu, Yusuf HakanPurpose: The use of Architect Syphilis TP (CMIA) in the blood bank raised the number of syphilis positive samples requiring confirmation. The aim of this study is to determine a sample-to-cutoff (s/co) ratio for CMIA predicting >= 95% of true-positive samples to reduce these samples. Methods: CMIA reactive samples (n=177) were evaluated by Western blot (WB) as the reference standard, as well as by Treponema pallidum hemagglutination (TPHA) and Rapid Plasma Reagin (RPR) tests. The s/co ratio predicting >= 95% of true-positive samples was defined as the threshold leaving >= 95% of WB confirmed samples greater than the particular value. The performances of TPHA and RPR tests were also evaluated with respect to s/co ratios of CMIA positive samples. Results: The s/co ratio 15.17 predicted a true-positive result for >= 95% of samples tested (95% confidence interval: 85.9-99.3) and reduced the number of samples requiring confirmation by 29.9%. Higher s/co ratios were correlated with the increasing number of bands on WB strips (p<0.0001, R=0.906). For the samples with s/co ratios between 3 and 15.17, the agreement of TPHA and WB test results were 90%. The lowest s/co ratio where TPHA was positive, was 3.1. Although RPR predicted > 95% of positive samples with s/co ratios > 15, its sensitivity was 47.7%. Conclusion: Higher s/co ratios can be used to define true-positivity and may indicate an active infection. TPHA may replace WB to confirm samples with s/co ratios between 3 and 15. RPR should not be used as a screening test in blood banks as it could miss almost half of the true-positive samples.