TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14365/4
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Article Expressions of the Satellite Repeat Hsat5 and Transposable Elements Are Implicated in Disease Progression and Survival in Glioma(Tubitak Scientific & Technological Research Council Turkey, 2024-08-23) Köse, Sıla Naz; Yaraş, Tutku; Bursalı, Ahmet; Oktay, Yavuz; Yandım, Cihangir; Karakulah, GökhanThe glioma genome encompasses a complex array of dysregulatory events, presenting a formidable challenge in managing this devastating disease. Despite the widespread distribution of repeat and transposable elements across the human genome, their involvement in glioma's molecular pathology and patient survival remains largely unexplored. In this study, we aimed to characterize the links between the expressions of repeat/transposable elements with disease progression and survival in glioma patients. Hence, we analyzed the expression levels of satellite repeats and transposons along with genes in low-grade glioma (LGG) and high-grade glioma (HGG). Endogenous transposable elements LTR5 and HERV_a-int exhibited higher expression in HGG patients, along with immune response-related genes. Altogether, 16 transposable elements were associated with slower progression of disease in LGG patients. Conversely, 22 transposons and the HSAT5 satellite repeat were linked to a shorter event-free survival in HGG patients. Intriguingly, our weighted gene coexpression network analysis (WGCNA) disclosed that the HSAT5 satellite repeat resided in the same module network with genes implicated in chromosome segregation and nuclear division; potentially hinting at its contribution to disease pathogenesis. Collectively, we report for the first time that repeat and/or transposon expression could be related to disease progression and survival in glioma. The expressions of these elements seem to exert a protective effect during LGG-to-HGG progression, whereas they could have a detrimental impact once HGG is established. The results presented herein could serve as a foundation for further experimental work aimed at elucidating the molecular regulation of glioma genome.Article Evaluation of Blood and Cerebrospinal Fluid Biochemistry, Cytology and Haematological Parameters in Head-And Form of Malignant Catarrhal Fever in Cattle(2023-08-01) Uzlu, Erdoğan; Erkılıç, Ekin Emre; Adalı, Yasemen; Öğün, Metin; Can Şahna, Kezban; Eryeğen, Nilhan; Eroğlu, Hüseyin Avni; Abayli, Hasan; Ermutlu, Celal ŞahinIn this study, it was aimed to examine the biochemical changes, hematological changes and cerebrospinal fluid (CSF) cytology and blood serum of cattle with head-eye form of Malignant Catarrhal Fever (MCF). For this pur- pose, 22 cattle diagnosed with "head-eye form" of MCF and clinically healthy 10 cattle were evaluated. Blood and cere- brospinal fluid (CSF) were collected from all cattle. In sera, AST, urea, glucose, CK (P<0.05), LDH levels (P<0.01) were found be high, ALT, ALP, cholesterol (P<0.05), Ca, total protein (P<0.01) and Mg, albumine and Fe levels (P<0.001) were found to be low in MCF group when compared to the control group. In CSF, Ca (P<0.01) and total protein levels (P<0.001) were found high glucose level (P<0.05) was found low in MCF group when compared to the control group. In haematology, some parameters were determined to be different between the groups. In cytological results of CSF in MCF group, polymorphonuclear leucocytes, lymphocytes, erytrocytes, macrophages and plasma cells were determined. In conclusion, since there were a limited number of studies examining biochemical, cytologic and hematological results of MCF especially in CSF, the results from our study were thought to be important for future stud- ies in which viral diseases affects the nervous system of cattles.Article Identification of the Role of Tg2 on the Expression of Tgf-Β, Timp-1 and Timp-2 in Aged Skin(Walter De Gruyter Gmbh, 2024-02-12) Ergülen, Elvan; Akdoğan, Gül; Guner, Gul AkdoganObjectives Transglutaminase 2 (TG2) is a unique protein having enzymatic and nonenzymatic functions that have been implicated in various biological and pathological processes such as cell survival and apoptosis, cell signaling, differentiation, adhesion and migration, wound healing and inflammation. As reported in previous studies, TG2 expression and activity increase by age suggesting that TG2 possibly has roles in cellular aging process. In this study, we aimed to explore the role of TG2 in chronological skin aging through its impact on the expression of some important extracellular matrix (ECM) proteins including TGF-beta, TIMP-1 and TIMP-2. Methods We have compared TG2 expression and activity in young and in vitro chronologically aged human dermal fibroblasts via Western blot and in situ TG2 activity assays. Afterwards, we inhibited TG2 expression via siRNA transfection and activity via active site inhibitor of TG2 separately in aged dermal fibroblasts and monitored the expression levels of TGF-beta, TIMP-1 and TIMP-2 in these cells by Western blot and compared to that of untreated control cells. Results We obtained evidence that both TG2 expression and activity increase in aged cells. However, protein levels of TGF-beta, TIMP-1 and TIMP-2 do not exhibit any significant difference in TG2 downregulated or TG2 activity inhibited aged cells compared to control cells. Conclusions Our results indicate that changes in the expression and activity of TG2 in (in vitro) chronologically aged human dermal fibroblasts do not impact the expression patterns of TGF-beta, TIMP-1 and TIMP-2 proteins.Article Experimental Intravaginal and Intrauterine Endometritis Model: Which Model Is More Useful?(2022-12-31) Beşeren, Hatice; Makav, Mustafa; Kuru, Mushap; Adalı, Yasemen; Coşkun, Mustafa Reha; Eroğlu, Hüseyin AvniThis study aims to compare the newly created intravaginal endometritis model (IVM) with the intrauterine endometritis model (IUM). E. coli infusion was used as intravaginally for IVM and intrauterinally for IUM model. The animals were exeuted on the 7th day. Histopathological and biochemical analyses [malondialdehyde (MDA), glutathione (GSH), Endocan, Endoglin] were performed. A significant inflammation was determined in IVM and IUM compared to the control. A significant decrease in GSH and a significant increase in MDA and Endoglin were determined in IVM and IUM compared to the control. There was a statistically significant increase in the IUM and a numerical increase in the IVM compared to the control. Endometritis was determined by histopathological and biochemical analyses in both IUM and IVM model. It is suggested that intravaginal administration, which is easier to perform, can be used in experimental endometritis model studies.Article Pathological and Biochemical Investigation of the Effects of L-Carnitine and Gemfibrozil on Peroxisome Proliferator Activated Receptors (ppars) and Lipidosis in Rabbits on a High-Fat Diet(2022-12-31) Erkılıc, Ekin Emre; Çitil, Mehmet; Tunca, Recai; Uzlu, Erdogan; Karapehlivan, Mahmut; Adalı, Yasemen; Yapar, Kürşad; Eroğlu, Huseyin Avni; Makav, MustafaObesity and fatty liver is a worldwide health problem in human with detrimental consequences where many investigations are undertaken to overcome this problem. In this study, gemfibrozil and L-carnitine were evaluated in prevention of obesity and lipidosis. The study involved 56 New-Zealand Albino rabbits, divided into 8 equal groups (n=7). The groups were as follow; group I (normal diet), II (normal diet +gemfibrozil), III (normal diet+L-carnitine) and IV (normal diet+gemfibrozil+L- carnitine), V (high fat diet), VI (high fat diet+gemfibrozil), VII (high fat diet+L- carnitine) and VIII (high fat diet+gemfibrozil+L-carnitine). Animals were blood sampled and wieght weekly during the experiment and at the end of the experiment for determination of biochemical parameters (glucose, total lipid). All rabbits were euthanised for histopathological examination and for distrubition of peroxisome proliferator activated receptors (PPARs) in tissues by immunohystochemistry. Gemfibrozil and L-carnitin treatment in rabbits given high fat diet resulted in statistically significant decrease in total lipid when compared to those only received high fat diet. Beta oxidation of high fat diet group was significantly higher than that of groups additionally received gemfibrozil and L-carnitine. Immunohistochemistry revealed an increase in PPAR, PPAR-α and β but not PPAR-γ expression in high fat diet group. On the contrary, L-carnitin administration had no effect on tissue PPAR expression. PPAR-α expression differed between groups received gemfibrozil and high fat diet and those did not. The most marked macroscopy finding was abdominal fat increase in high fat diet group (group V). On the other hand gemfibrozil administration resulted in significant abdominal fat decrease. Furthermore decreased abdominal fat was marked in gemfibrozil and L-carnitine given animals (group VIII) when compared to other groups. In conclusion, gemfibrozil and L-carnitine administration alleviated abdominal and hepatic fattening. Gemfibrozil also caused a significant increase in PPAR-α expression in the liver. It may be of use in avoiding abdominal fat (obesity) due to high fat diet by use of gemfibrozil, a synthetic PPAR-a ligand, and L-carnitine.Article Investigation of Cyclin-D1 Immunohistochemical Expression in Bladder Urethelial Carcinoma(2023-06-27) Adalı, Yasemen; Ezer, Mehmet; Yılmaz Ertürk, Fatma; Beşeren, Hatice; Ertürk, Fatma YılmazObjective: Cyclin D1 is a protein that is involved in the regulation of the cell cycle and is encoded by the CCND1 gene. There are few studies on Cyclin D1 in the literature, and the results differ in the studies. In this study, the relationship between Cyclin D1 expression and prognostic factors in bladder urothelial carcinomas was investigated. Materials and Methods: Forty-six patients who underwent TUR-M at the Kafkas University Health Research and Application Hospital were included in the study. General information about the cases and pathology reports were obtained from the hospital automation system. Tumor-containing sections were selected from the Hematoxylin and Eosin stained pathology slides, and immunohistochemical staining was performed manually with Cyclin D1 primary antibody on the blocks of the selected slides. Immunostained pathology slides were evaluated under light microscope by scoring 0-4 separately as nuclear and cytoplasmic scores. Results: The age range of the cases was 51-93, and the mean age was 69.2±11.7. Twelve (26.1%) cases were female and 34 (73.9%) were male. It was observed that 29 (63.0%) of the cases were low- grade and 17 (37.0%) were high-grade. Eighteen (39.1%) of the cases were invasive and 28 (60.9%) were noninvasive. In the statistical analyzes, it was noted that invasive tumors had a significantly higher grade compared to non-invasive tumors (pTa) (p= 0.007). Similarly, the presence of lymphovascular invasion in invasive tumors was statistically <0.005 higher than that of in non- invasive tumors. (p=0.001). It was observed that nuclear cyclin D1 expression (p=0.003) was significantly higher in invasive cases. In addition, nuclear cyclinD1 expression was found to be statistically significantly higher in low-grade tumors (p=0.044). Conclusion: As a result of the study, a relationship between Cyclin D1 expression and tumor grade and invasion status was observed in patients with bladder urothelial carcinoma, but studies with larger case series are needed to use Cyclin D1 as a biomarker.Article ?-Hydroxybutyrate Does Not Influence Viability and Clonogenicity of A549 Lung Cancer Cells(2023-03-15) Özkaya, Ali Burak; Malcanlı, Semanur; Geyik, Öykü GönülBackground/Purpose: The metabolic shift from catabolism of carbohydrates to lipids results in production of ketone bodies leading to a state called ketosis. Ketosis via ketone supplement or ketogenic diet has been proposed as a non-toxic therapeutic option for a broad range of malignancies. Although the clinical impact of ketogenic diet is well-documented, the effect of ketone bodies on cancer cell biology is not clear for some cancers including non-small-cell lung cancer (NSCLC). In this study, we aimed to demonstrate the effects of the most prominent ketone body, ?-hydroxybutyrate, on a NSCLC cell line, A549. Methods: A549 cell line was utilized as the in vitro model in this study. The effects of different ?-hydroxybutyrate concentrations on cell viability were measured via sulphorodamine-B (SRB) viability assay. Long term effects of ketosis were evaluated via colony formation assay. Finally, the effect of ?-hydroxybutyrate on cell migration was determined via scratch assay. Results: Our results suggest that introduction of ?-hydroxybutyrate in physiologically relevant concentrations into the cell culture media does not influence cell viability, clonogenicity or migration. Conclusion: ?-hydroxybutyrate has been previously demonstrated to induce, inhibit or does not influence the viability of different cell lines but there is no report regarding its effects on NSCLC cells. Here we report that physiologically relevant concentrations of ?-hydroxybutyrate have no effect on viability, clonogenicity and migration of A549 cells.