Keleş Bartık, Didem
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Bartık, Didem Keleş
Keles, Didem
Keles, D.
Keles Bartik, Didem
Keles, Didem
Keles, D.
Keles Bartik, Didem
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didem.keles@ieu.edu.tr
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15.06. Medical Laboratory Techniques
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Current Staff
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1NO POVERTY
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2ZERO HUNGER
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3GOOD HEALTH AND WELL-BEING
8
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4QUALITY EDUCATION
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5GENDER EQUALITY
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6CLEAN WATER AND SANITATION
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7AFFORDABLE AND CLEAN ENERGY
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8DECENT WORK AND ECONOMIC GROWTH
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9INDUSTRY, INNOVATION AND INFRASTRUCTURE
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10REDUCED INEQUALITIES
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11SUSTAINABLE CITIES AND COMMUNITIES
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12RESPONSIBLE CONSUMPTION AND PRODUCTION
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13CLIMATE ACTION
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14LIFE BELOW WATER
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15LIFE ON LAND
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16PEACE, JUSTICE AND STRONG INSTITUTIONS
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Documents
12
Citations
143
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7
Documents
29
Citations
133
Scholarly Output
12
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10
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66/89
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0
WoS Citation Count
23
Scopus Citation Count
28
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WoS Citations per Publication
1.92
Scopus Citations per Publication
2.33
Open Access Source
6
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0
| Journal | Count |
|---|---|
| Journal of Basic and Clinical Health Sciences | 3 |
| Chemico-Biological Interactions | 1 |
| Chemıco-Bıologıcal Interactıons | 1 |
| Ent Updates | 1 |
| FEBS Open Bio | 1 |
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12 results
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Now showing 1 - 10 of 12
Article 1,25-Dihydroxyvitamin D3 Induces N-Myc Downstream Regulated Gene-2 Expression In Papillary Thyroid Carcinoma Cells(2020) Sipahi, Murat; Keleş, Didem; Oktay, Gulgun; Bayraktar, Fırat; Doruk, MehmetPurpose: In addition to its role in serum calcium homeostasis, the anti-tumor function of 1,25-dihydroxyvitamin D3 (calcitriol) in cancer development is well established. N-myc Downstream Regulated Gene 2 which functions as a tumor suppressor gene has recently been shown to be downregulated in various cancer leading to increased tumor incidence, progression and metastasis. The goal of this study was to investigate the possible effects of calcitriol treatment on NDRG2 expression in BCPAP papillary thyroid carcinoma cells. Methods: The experiments were carried on human primary thyroid follicular epithelial cells (Nthy-ori-3-1), and human papillary thyroid carcinoma cells (BCPAP). The half maximal inhibitory concentration (IC50) of calcitriol on BCPAP cells was determined by WST-1 assay. BCPAP cells were treated with 15 and 30µM calcitriol for 24, 48, and 72 hours, respectively. Basal NDGR2 expression in Nthy-ori-3–1 and BCPAP cells as well as the alterations on NDRG2 expression in calcitriol treated BCPAP cells were evaluated with western blot. Results: A significant downregulation of NDRG2 was observed in BCPAP cells when compared to Nthy-ori-3–1 cells (p<0.01). IC50 dose of calcitriol was found to be 64, 54 and 43µM for 24, 48 and 72 hours, respectively. NDRG2 protein expression levels were significantly increased in 30µM calcitriol treated BCPAP cells after 48 hours (p<0.05). Conclusions: Calcitriol induced NDRG2 protein expression in BCPAP cells. We predict that calcitriol increased NDRG2 protein levels in BCPAP cells via c-Myc repression, which is upregulated by aberrant Wnt/β-catenin signaling. Further investigation is required to enlighten the possible effect mechanisms of calcitriol in BCPAP cells.Article 1,25-Dihydroxyvitamin D3 Induces N-Myc Downstream Regulated Gene-2 Expression in Papillary Thyroid Carcinoma Cells(Dokuz Eylul Univ Inst Health Sciences, 2020) Sipahi, Murat; Bartik, Didem Keles; Doruk, Mehmet; Bayraktar, Firat; Oktay, GulgunPurpose: In addition to its role in serum calcium homeostasis, the anti-tumor function of 1,25-dihydroxyvitamin D-3 (calcitriol) in cancer development is well established. N-myc Downstream Regulated Gene 2 which functions as a tumor suppressor gene has recently been shown to be downregulated in various cancer leading to increased tumor incidence, progression and metastasis. The goal of this study was to investigate the possible effects of calcitriol treatment on NDRG2 expression in BCPAP papillary thyroid carcinoma cells. Methods: The experiments were carried on human primary thyroid follicular epithelial cells (Nthy-ori-3-1), and human papillary thyroid carcinoma cells (BCPAP). The half maximal inhibitory concentration (IC 50) of calcitriol on BCPAP cells was determined by WST-1 assay. BCPAP cells were treated with 15 and 30 mu M calcitriol for 24, 48, and 72 hours, respectively. Basal NDGR2 expression in Nthy-ori-3-1 and BCPAP cells as well as the alterations on NDRG2 expression in calcitriol treated BCPAP cells were evaluated with western blot. Results: A significant downregulation of NDRG2 was observed in BCPAP cells when compared to Nthy-ori-3-1 cells (p<0.01). IC50 dose of calcitriol was found to be 64, 54 and 43 mu M for 24, 48 and 72 hours, respectively. NDRG2 protein expression levels were significantly increased in 30 mu M calcitriol treated BCPAP cells after 48 hours (p<0.05). Conclusions: Calcitriol induced NDRG2 protein expression in BCPAP cells. We predict that calcitriol increased NDRG2 protein levels in BCPAP cells via c-Myc repression, which is upregulated by aberrant Wnt/beta-catenin signaling. Further investigation is required to enlighten the possible effect mechanisms of calcitriol in BCPAP cells.Article Citation - WoS: 2Citation - Scopus: 3Effects of Vertebral Fusion on Levels of Pro-Inflammatory and Catabolic Mediators in a Rabbit Model of Intervertebral Disc Degeneration(Turkish Assoc Orthopaedics Traumatology, 2021-05-27) Dumanlidag, Davut; Keles, Didem; Oktay, Gulgun; Kosay, CanObjective: The aim of this study was to explore the alterations in levels of pro-inflammatory and catabolic mediators following vertebral fusion in a rabbit model of intervertebral disc degeneration. Methods: In this study, 24 female New Zealand albino rabbits (aged 4 to 5 months and weighing 3 to 3.5 kg) were used. All the animals were randomly categorized into four groups, and dorsal spinal exposure of all lumbar vertebrae was routinely performed in each group. While disc degeneration was created in groups B, C, and D, spinal fusion was added to disc degeneration in groups C and D. Disc degeneration was typically created by puncturing the discs with an 18-gauge needle under the guidance of C-arm imaging. Fusion was achieved with posterior/posterolateral decortication and iliac bone grafts. The rabbits in groups A, B, and C were euthanized, and the discs were removed in the first week after the surgery. The rabbits in Group D were sacrificed, and the discs were harvested at 5 weeks after the surgery. The levels of Interleukin (IL)-1 beta, IL-6, Nitric Oxide (NO), Matrix Metalloproteinase (MMP)-3, MMP-13, and Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) in the discs were analyzed using enzyme-linked immunosorbent assay kits. Results: Significant increase was observed in the protein levels of both pro-inflammatory and catabolic mediators in disc degeneration groups (Group B, C, and D) compared to Group A. In the fusion groups (Group C and D), these increased mediators decreased, compared to non-fusion group (Group B), (IL1-beta P = 0.017, TIMP-1 P = 0.03, NO P = 0.03). However, there was no statistically significant difference in mediator levels between the short- and long-term fusion (Group C versus D). Conclusion: The results of this study have shown that a significant decrease in pro-inflammatory and catabolic mediators may be expected after vertebral fusion whereas there may be no significant difference between the first and fourth week of fusion surgery. These findings may contribute to clarifying the mechanism of action of vertebral fusion in the treatment of low back pain.Conference Object The Effects of Metabolic Drugs on Tumor Behaviour in Oral Squamous Cell Carcinoma(Wiley, 2021) Inanc-Surer, S.; Keleş Bartık, Didem; Sipahi, M.; Oktay, G.; Keles, D.[Abstract Not Available]Article Aconitine Impedes Cell Motility in Mda-Mb Breast Cancer Cells(Dokuz Eylul Univ Inst Health Sciences, 2024-09-30) Keles, Didem; Sipahi, Murat; Surer, Seniz Inanc; Oktay, GulgunPurpose: Aconitine, a potent alkaloid from Aconitum plants, has shown promising anticancer properties. The aim of the study is to investigate the effects of aconitine on lateral migration, and matrix metalloproteinase (MMP) activity in MDA-MB-231 triple-negative breast cancer cells. Material and Methods: A WST-1 viability assay was conducted to determine the effect of aconitine on the viability of MDA-MB-231 cells. Following treatment with non-cytotoxic doses of aconitine, lateral migration was evaluated through wound healing assays. Additionally, gelatin zymography was conducted to analyze MMP-2 and MMP-9 activity and secretion levels. Results: Aconitine concentrations up to 200 mu M did not significantly affect cell viability for up to 72 hours, whereas higher doses (400-600 mu M) reduced viability in a time-dependent manner. Aconitine at 200 mu M showed a trend towards decreased lateral motility, with a significant reduction at 9 hours post-treatment. Gelatin zymography revealed no alterations in MMP-2 and MMP-9 activity or secretion levels following aconitine treatment. Conclusion: Aconitine demonstrates limited efficacy in modulating the migratory capacity of MDA-MB231 cells and does not affect gelatinase activity. Further investigation into underlying mechanisms is necessary, potentially leading to novel therapeutic strategies for triple-negative breast cancer.Article Citation - WoS: 2Metformin and Dichloroacetate Combination Exert a Synergistic Effect on Cell Viability of Oral Squamous Cell Carcinoma(Deomed Publ, Istanbul, 2019-05-29) Inanc, Seniz; Keles, Didem; Eskiizmir, Gorkem; Basbinar, Yasemin; Oktay, GulgunObjective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line. Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method. Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells. Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the drug repositioning of the treatment of oral cavity cancer.Article Citation - WoS: 8Citation - Scopus: 10Effects of Simvastatin on Matrix Metalloproteinase Regulation in Il-1 Beta-Induced Sw1353 Cells(Elsevier Ireland Ltd, 2019-09) Cecen, Berivan; Keles, Didem; Oktay, Gulgun; Kozaci, Leyla DidemThe present study shows the basis for the anti-inflammatory effects of statins in interleukin 1 beta (IL-15) induced SW1353 chondrosarcoma cell-line. The cells were pre-treated with simvastatin (5 mu M, 10 mu M, and 50 mu M), followed by IL-1 beta (5 ng/mL) stimulation. Effects of simvastatin on cell viability and cytotoxicity of chondrocytes were measured with WST-1 and lactate dehydrogenase (LDH) assays, respectively. Under inflammatory conditions, in the absence/presence of simvastatin, the changes in matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase-1 (TIMP-1) expression levels were examined. Expression levels of MMP-1, -2, -3, -9, -13, and TIMP-1 and -2 were examined by qPCR. MMP-1, -9, -13, TIMP-1, and -2 levels were also determined by Western blotting. Gelatin zymography was performed to analyze the released and intracellular MMP-2 and MMP-9 activity levels. The results showed that simvastatin downregulated the degradation related genes MMP3, MMP-13, MMP-2, MMP-9 and TIMP-2 in a dose-dependent manner.Article Citation - WoS: 4Citation - Scopus: 4Tetracaine Downregulates Matrix Metalloproteinase Activity and Inhibits Invasiveness of Strongly Metastatic Mda-Mb Human Breast Cancer Cells(Elsevier Ireland Ltd, 2023-11) Keleş Bartık, Didem; Sipahi, M.; İnanç-Sürer; Djamgoz, M.B.; Oktay, G.; Keleş, Didem; İnanç-Sürer, ŞenizTetracaine, a long-acting amino ester-type local anesthetic, prevents the initiation and propagation of action potentials by reversibly blocking voltage-gated sodium channels (VGSCs). These channels, which are highly expressed in several carcinomas (e.g. breast, prostate, colon and lung cancers) have been implicated in promoting metastatic behaviours. Recent evidence suggests that local anesthetics can suppress cancer progression. In this paper, we aimed to explore whether tetracaine would reduce the invasive characteristics of breast cancer cells. In a comparative approach, we used two cell lines of contracting metastatic potential: MDA-MB-231 (strongly metastatic) and MCF-7 (weakly metastatic). Tetracaine (50 μM and 75 μM) did not affect the proliferation of both MDA-MB-231 and MCF-7 cells. Importantly, tetracaine suppressed the migratory, invasive, and adhesive capacities of MDA-MB-231 cells; there was no effect on the motility of MCF-7 cells. Tetracaine treatment also significantly decreased the expression and activity levels of MMP-2 and MMP-9, whilst increasing TIMP-2 expression in MDA-MB-231 cells. On the other hand, VGSC α/Nav1.5 and VGSC-β1 mRNA and protein expression levels were not affected. We conclude that tetracaine has anti-invasive effects on breast cancer cells and may be exploited clinically, for example, in surgery and/or in combination therapies. © 2023 Elsevier B.V.Article A Simple and Efficient "Cell in Situ Collagen Zymography" Technique To Evaluate Cellular Collagenase Activities in Thyroid Cancer Cell Lines(Springer, 2024-12-14) Savas, Ege Gokce; Surer, Seniz Inanc; Sipahi, Murat; Keles, Didem; Oktay, GulgunBackgroundCollagenases, a subgroup of matrix metalloproteinases (MMPs), play crucial roles in local invasion and metastasis in cancer. While substrate zymography and in situ zymography are commonly used to analyze the collagenases, traditional techniques have limitations in determining their local activities in vitro.ObjectivesWe aimed to develop a new "cell in situ collagen zymography" technique to enhance the efficiency of studying local collagenase activities in vitro.MethodsWe utilized human thyroid cancer cell lines (8505 C, B-CPAP, FTC-133) and normal follicular thyroid cell line (Nhty-ori-3-1). We compared collagenase levels across these cell lines and selected 8505 C as a model due to its highest collagenase activity. We optimized factors including (i) fixation method (methanol, ethanol and zinc), (ii) dye-quenched (DQ) collagen concentration and (iii) collagen gel configuration. For gel configuration, cells were seeded under, on the top of, or between (sandwich) collagen gel layers. As controls, enzymatic activity was suppressed in the presence of EDTA, piroxicam and matrix metalloproteinase 8 inhibitor I. The optimized method was also applied to BCPAP, FTC-133, and Nthy-ori-3-1.ResultsOur optimization process revealed that that the best visualization of collagenase activity in 8505 C was provided by the "sandwich model" of gel, containing 25 mu g/mL of DQ-collagen with 100% cold methanol fixation. We confirmed the optimized method's applicability in other thyroid cell lines. The use of inhibitors validated the specificity of the fluorescent signal to MMP activity.ConclusionThe innovative "cell in situ collagen zymography" technique offers an efficient, cost-effective, and rapid method for analyzing local collagenase activities in vitro.Conference Object The Antidepressant Sertraline Impairs Proliferation and Invasiveness of Human Glioblastoma Cells(Wiley, 2025) Keles, D.; Inanc-Surer, S.; Sipahi, M.; Erkan, E. P.; Ates, H.; Erbayraktar, Z.; Oktay, G.
