Barış, Elif
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Baris, Elif
Baris, E.
Baris, E.
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elif.baris@ieu.edu.tr
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09.01. Basic Medical Sciences
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11
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8
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12
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15
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16
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3
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3
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16
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47
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3
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26
Citations
53
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30
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24
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70/157
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1
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53
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47
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4
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1.77
Scopus Citations per Publication
1.57
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16
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1
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| Journal | Count |
|---|---|
| Acta Physıologıca | 3 |
| Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | 3 |
| Bratislava Medical Journal | 2 |
| Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi | 1 |
| Clınıcal And Experımental Health Scıences | 1 |
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30 results
Scholarly Output Search Results
Now showing 1 - 10 of 30
Article Effect of Choline and Cdp-Choline on Inflammation and Oxidative Stress in Burkitt's Lymphoma Cells(Asian Network Scientific information-ansinet, 2025) Roshani, Shideh; Baris, Elif; Bosnak, Ahmet Sami; Gali-Muhtasib, Hala; Hamurtekin, EmreBackground and Objective: Burkitt's lymphoma (BL) is a specific type of non-Hodgkin lymphoma. The BL is characterized by rapid progression and a tendency to metastasize the bone marrow and central nervous system. This study aims to evaluate the anticancer potential of choline and CDP-choline on BL cells (Ramos cells), in vitro. Materials and Methods: Ramos cells were treated with increasing concentrations of doxorubicin, choline and CDP-choline for 24 hrs after which cell viability was assessed using the MTT assay. Cytokine levels (IL-6 and TNF-") and reactive oxygen species (ROS) production were measured using ELISA and fluorometric kits, respectively. One-way Analysis of Variance (ANOVA) with post hoc Tukey-Kramer multiple comparison tests were used for the statistical analysis, p<0.05 was accepted as a statistically significant level. Results: Choline and CDP-choline treatment for 24 hrs decreased Ramos cell viability, with IC50 values of 100, 02 and 5.45 M, respectively. Both treatments increased ROS levels, indicating induction of oxidative stress. However, treatment of Ramos cells with these agents for 24 hrs did not induce cytokines (IL-6 and TNF-") production. Choline treatment increased supernatant choline levels, whereas CDP-choline had no significant effect on intracellular choline in Ramos cells. Conclusion: Choline and CDP-choline reduced cell viability of Ramos cells probably via ROS dependent mechanism, but did not induce inflammatory responses at 24 hrs post-treatment.Thesefindings suggested the possible anticancer potential ofcholine and CDP-choline against BL. This warrants further investigation into their potential therapeutic implications.Article Citation - WoS: 2Citation - Scopus: 2Right Vagotomy Alters Heart Rate Variability Temporarily and Increases Total Choline Levels in Rats(Walter de Gruyter GmbH, 2024) Barış, Elif; Ozel, Hasan Fehmı; Kazdağlı, Hasan; Özbek, MustafaObjectives: The variability in the time intervals between heartbeats, known as heart rate variability (HRV), serves as a reflection of the intricate interplay between the sympathetic and parasympathetic neural systems. While the potential asymmetric effects of the left and right branches of the vagus nerve remain uncertain, this study aims to investigate the impact of unilateral, bilateral, and atropine interventions on HRV parameters and choline levels within cardiac tissue. Methods: 40 male adult Wistar albino rats were randomly assigned to the five groups (each n=8): sham-operated, atropine, right vagotomy, left vagotomy, and bilateral vagotomy. Heart rate variability (HRV) analyses were conducted, and the levels of total choline/acetylcholine in heart tissues were quantified. Statistical analyses were performed to assess the results. Results: The bilateral vagotomy and atropine groups exhibited higher heart rates and high frequency power (HF), along with reduced low frequency power (LF). Total power (TP) remained relatively unchanged. In the bilateral vagot- omy group, DFAα1 was significantly elevated while DFAα2 was reduced significantly. SD1 and SampEn were significantly lower in both the bilateral vagotomy and atropine groups. Notably, the right vagotomy group displayed significant changes primarily in the 15th minute, particularly in time- domain parameters, HF, TP, and SD1, with a significant in- crease observed in total choline levels. Conclusions: Our results revealed that asymmetrical vagal innervation induces distinct effects on heart rate variability parameters and total choline/acetylcholine levels in heart tissues. Our findings suggest that compensatory hemody- namic recovery, possibly driven by contralateral vagal overactivity, may contribute to these observed results.Master Thesis Α7 Nachr Aracılıklı Kolinerjik Anti-inflamatuar Yolak Aktivasyonunun Regorafenib Direncine Etkisinin Belirlenmesi(2025) Sezan, Sıla; Karagonlar, Zeynep Fırtına; Barış, ElifHepatoselüler karsinom (HCC), agresif doğası, yüksek metastatik potansiyeli ve sınırlı tedavi seçenekleri nedeniyle küresel bir sağlık sorunu olmaya devam etmektedir. İleri evre HCC için ikinci basamaktaki tedavi olan regorafenib, umut vaat etse de, direnç mekanizmaları nedeniyle etkinliği sınırlıdır. Ortaya çıkan kanıtlar, tümör mikroçevresinin (TME), inflamasyonun ve tümörle ilişkili makrofajların (TAM'ler) direnç mekanizmalarındaki kritik rolünü vurgulamaktadır. Bu çalışma, HCC'de regorafenib direncinin üstesinden gelmede kolinerjik anti-inflamatuar yolak (CAP) içindeki α7 nikotinik asetilkolin reseptörünün (α7 nAChR) ve aktivasyonunun rolünü araştırmaktadır. Regorafenib dirençli Huh7 (RRC) hücrelerini ve polarize M1 ve M2 makrofajlarını içeren 2D kültür ve 3D sferoid modelleri kullanarak, regorafenib ile kombine edilen bir α7 nAChR ligandı olan kolinin etkilerini inceledik. Bulgularımız, kolinin regorafenibin etkinliğini artırdığını, RRC hücrelerinin canlılığını, migrasyonunu ve çoğalmasını azalttığını, aynı zamanda M1 makrofaj polarizasyonunu teşvik ettiğini göstermektedir. RRC hücrelerinde artmış α7 nAChR ekspresyonu ve kalsiyum alışverişi gözlemlendi, bu da direnç mekanizmalarındaki kritik rolünü ortaya koymaktadır. Ek olarak, kombine tedavi, NF-κB dahil olmak üzere temel inflamatuar ve onkojenik yolları önemli ölçüde regüle etti. Bu sonuçlar, HCC'de regorafenib direncinin aşılması için α7 nAChR'yi hedef almanın terapötik potansiyelini vurgulamakta ve ileri evre HCC hastaları için klinik sonuçları iyileştirebilecek yeni bir kombinasyon stratejisi önermektedir.Article Enhancement of Corchorus olitorius L. on Osteogenic Differentiation of MC3T3-E1 Pre-Osteoblast Cells by Increasing Alkaline Phosphatase and Hydroxyproline(Taylor & Francis Ltd, 2025) Ertugruloglu, Pinar; Baris, Elif; Okkali, Gaye Sumer; Boke Sarikahya, NazliCorchorus olitorius L. (jute mallow or molehiya) belongs to the Malvaceae family valued for its nutritional and medicinal properties. In this study, the potential to enhance osteogenesis in MC3T3-E1(Murine Calvaria-derived 3T3 Subclone E1) pre-osteoblastic cells was investigated to support bone formation and mineralisation. Leaf ethanolic extract was prepared and applied to MC3T3-E1 cells. Osteogenic effects were evaluated through three methods: MTT assays for cell viability, Alizarin Red S staining for calcium deposition, enzymatic analyses for alkaline phosphatase (ALP) and hydroxyproline (HYP). A non-cytotoxic concentration of C. olitorius extract (0.5 mg/mL) significantly increased ALP and HYP levels, promoting osteogenic differentiation in both undifferentiated and differentiated cells. HYP levels were notably elevated in differentiated cells. The findings suggested that C. olitorius extract may be a promising natural agent for enhancing bone health, warranting further in vivo and clinical studies to confirm its therapeutic potential.Article Citation - WoS: 1Citation - Scopus: 1Toll-Interacting Protein May Affect Doxorubicin Resistance in Hepatocellular Carcinoma Cell Lines(Springer, 2023) Demir, Ayşe Banu; Barış, Elif; Kaner, Umay Bengi; Alotaibi, Hani; Atabey, Nese; Koc, AhmetBackgroundLiver cancer is the third leading cause of cancer-related deaths worldwide, and hepatocellular carcinoma (HCC) is the most common type of liver cancer. Transarterial interventions are among the chemotherapeutic approaches used in hardly operable regions prior to transplantation, and in electrochemotherapy, where doxorubicin is used. However, the efficacy of treatment is affected by resistance mechanisms. Previously, we showed that overexpression of the CUE5 gene results in doxorubicin resistance in Saccharomyces cerevisiae (S. cerevisiae). In this study, the effect of Toll-interacting protein (TOLLIP), the human ortholog of CUE5, on doxorubicin resistance was evaluated in HCC cells to identify its possible role in increasing the efficacy of transarterial interventions.Methods and resultsThe NIH Gene Expression Omnibus (GEO) and Oncomine datasets were analyzed for HCC cell lines with relatively low and high TOLLIP expression, and SNU449 and Hep3B cell lines were chosen, respectively. TOLLIP expression was increased by plasmid transfection and decreased by TOLLIP-siRNA in both cell lines and evaluated by RT-PCR and ELISA. Cell proliferation and viability were examined using xCELLigence and MTT assays after doxorubicin treatment, and growth inhibitory 50 (GI 50) concentrations were evaluated. Doxorubicin GI 50 concentrations decreased approximately 2-folds in both cell lines upon silencing TOLLIP after 48 h of drug treatment.ConclusionsOur results showed for the first time that silencing TOLLIP in hepatocellular carcinoma cells may help sensitize these cells to doxorubicin and increase the efficacy of chemotherapeutic regimens where doxorubicin is used.Article Effects of Varenicline as an Adjunct to Analgesic and Anti-Inflammatory Therapy in Acute Nerve Injury(Elsevier Sci Ltd, 2026) Ozturk, Volga; Rusen, Yasemen Adali; Ertener, Ozge; Seval-Celik, Yasemin; Dastan, Ali Engin; Ozgenc, Serhat; Baris, ElifIntroduction: Acute nerve injury (ANI) leads to significant neuropathic pain and functional impairment. Current treatments, including nonsteroidal anti-inflammatory drugs (NSAIDs) like meloxicam, provide symptomatic relief but have limited neuroregenerative effects. Varenicline, a nicotinic acetylcholine receptor (nAChR) agonist, has demonstrated neuroprotective and anti-inflammatory properties. Aim: This study evaluates the effects of varenicline as an add-on therapy to meloxicam in a rat model of ANI. Methods: Eighteen female Wistar rats were randomized into four groups: Control (CONT), Sham (SHAM), Acute Nerve Injury + Meloxicam (ANI+Melox), and Acute Nerve Injury + Meloxicam + Varenicline (ANI+Melox+VAR). Varenicline (2.5 mg/kg, s.c.) was administered alongside meloxicam (2 mg/kg, s.c.). Functional recovery, histopathological changes, and biochemical markers, including prostaglandins (PGE2, PGI2), substance P, IL-6, levels, were assessed after 30 days. Results: Varenicline and meloxicam co-treatment significantly reduced inflammatory and pain biomarkers including prostaglandins, interleukin-6 and substance P, compared to meloxicam alone. Histopathological evaluation revealed enhanced Schwann cell proliferation, reduced fibrosis, and increased Bands of B & uuml;ngner formation, suggesting nerve regeneration. Conclusion: Varenicline, as an adjunct to meloxicam, enhances neuroprotection, reduces inflammation, and promotes histological and biochemical indicators of regeneration in rats with acute sciatic nerve injury. Future studies should explore its long-term effects and potential as a monotherapy for peripheral nerve injuries.Article Physiological and Inflammatory Changes to Natural and Skin-Related Trypophobic Images(Akademiai Kiado Zrt, 2025) Kazdagli, Hasan; Baris, Elif; Kipcak, Arda; Ozturk, Suleyman; Ceylan, Deniz; Demir, Ayse Banu; Erdeniz, BurakIntroduction: The immune system's inflammatory response, driven by pro-inflammatory proteins, protects against external threats. Fear and disgust-inducing stimuli have been linked to immune responses, yet their specific physiological and inflammatory mechanisms in trypophobia remain unclear. This study aimed to elucidate the inflammatory and physiological responses in relation to natural (non-skin) and skin-related trypophobic images. Material and methods: Fifty participants (n = 50) were recruited for the study, and their sensitivity to trypophobia was measured using the trypophobia questionnaire. Then, participants were randomly assigned to either the skin related or non-skin related visual exposure group and viewed trypophobic images from a computer screen. Blood samples were collected pre- and post-exposure to trypophobic images and analyzed for IL-6 and TNF-alpha using ELISA and RT-qPCR methods. Results: IL-6 and TNF-alpha protein levels significantly increased post-exposure, with IL-6 changes varying by stimulus type. mRNA expression showed significant interaction with participants' trypophobia sensitivity scores, suggesting post-transcriptional mechanisms. Heart rate variability (HRV) and heart rate were measured before, during, and after exposure using photoplethysmography. Significant changes in HRV metrics, influenced by stimulus type and trypophobia sensitivity, indicated increased sympathetic and decreased parasympathetic nervous system activity during and after exposure. Conclusions: These findings highlight the role of physiological and inflammatory responses in trypophobia, suggesting immune activation and autonomic nervous system involvement based on stimulus type and individual sensitivity. These findings not only contribute to phobia literature but also shed light on the physiological and immunological changes that take place in the bodies of individuals with high sensitivity to trypophobia.Article Complications and Therapeutic Approaches in a Sciatic Nerve Injury Rat Model(2025) Adali, Yasemen; Barış, Elif; Daştan, Ali Engin; Öztürk, VolgaSciatic nerve injury (SNI) is a common model for studying peripheral nerve damage and regeneration. This study investigates the complications associated with acute nerve injury (ANI) by laceration of sciatic nerve in rats including infection, edema, and cannibalism, and evaluates the effectiveness of therapeutic interventions to modulate the observed complications. For this purpose eighteen female wistar albino rats were divided into three groups: control, sham-operated, and ANI. The ANI model induced with dissection and repair of the right sciatic nerve. Post-surgical care included the administration of diclofenac sodium for pain management. Observations were made for signs of infection, edema, hematoma, and survival rates within 10 days. The ANI group showed significant complications, including a 41.6% incidence of symptoms of pain (paraesthesia, allodynia, hyperalgesia, decreased activity, piloerection, excessive licking, un-groomed appearance) within 3 days, which increased to 60% by day 5. Edema was observed in 8.3% of the ANI rats, and 33.3% developed hematomas. Cannibalism rates also increased, particularly within 10 days post-injury. Survival rates in the ANI group decreased to 16.6% by day 10, indicating severe post-operative complications. The current study highlights the critical complications associated with ANI in rats, particularly the high rates of pain related symptoms (i.e. paresthesia and cannibalism). These findings suggest the need for improved post-operative care and highlight the importance of therapeutic interventions like opioid analgesics to mitigate these complications and enhance recovery outcomes in peripheral nerve injury models.Article Citation - WoS: 1Citation - Scopus: 1Differential Effects of Choline on TLR2/4 Mediated Signaling Through Possible Regulation of Toll-Interacting Protein in Hepatocellular Carcinoma Cell Lines(Walter de Gruyter GmbH, 2024) Barış, Elif; Demir, Ayse BanuObjectives: Toll-like receptor (TLR) mediated inflammatory status plays an important role in development and pro- gression of hepatocellular carcinoma (HCC). Toll-interacting protein (TOLLIP) has an inhibitory effect on TLR-mediated inflammatory signalling and expression profile of TOLLIP varies between malignancies including HCC. Cholinergic anti-inflammatory pathway (CAP) is an endogenous mech- anism that controls inflammatory status via α7nicotinic acetylcholine receptors (α7nAChR). This study aims to investigate the effect of CAP-acting agent choline on TOLLIP and its related TLR-mediated inflammatory response in HCC cells with distinct differentiation stages. Methods: The expression patterns of α7nAChR, TLR2/4, TOLLIP, IL6, NFkB genes were evaluated by RT-PCR and ELISA in the presence of choline, along with the real-time cell proliferation and migration in HEP3B and SNU449 HCC cell lines. The interaction between choline and TOLLIP assessed by using in-silico analyses. Results: Choline downregulated TOLLIP in Hep3B and SNU449 cells. However, the expressions of α7nAChR, NF-κB, IL-6, TLR2 and TLR4 showed a decreased pattern in well differentiated HEP3B cells, while an increased pattern in poorly differentiated SNU449 cells. Conclusions: Choline might exert differential effects in TLR2/4-dependent signalling based on the differentiation stages of the HCC cells, suggesting its potential therapeutic effects in earlier stages of HCC which might be result of its partial modulation of TOLLIP.Conference Object Changes in Total Choline Levels in Heart Tissues of Vagotomized Rats.(Wiley, 2022) Kazdağlı, Hasan; Ozel, H. F.; Barış, Elif; Ozbek, M.[Abstract Not Available]
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