Rapid Fibroblast Cell Culture Characterization With Impedimetric Label-Free Heat Shock Protein-47 Biosensor
| dc.contributor.author | Harmancı, Duygu | |
| dc.contributor.author | Uygun, Zihni Onur | |
| dc.contributor.author | Sezgin, Ayse Kocak | |
| dc.contributor.author | Demirdover, Cenk | |
| dc.contributor.author | Sagin, Ferhan Girgin | |
| dc.contributor.author | Akdogan, Gul | |
| dc.contributor.author | Koçak Sezgin, Ayşe | |
| dc.contributor.author | Onur Uygun, Zihni | |
| dc.contributor.author | Girgin Sagin, Ferhan | |
| dc.date.accessioned | 2023-06-16T12:47:33Z | |
| dc.date.available | 2023-06-16T12:47:33Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | In this study, for the first time in literature, an impedimetric biosensor technology was developed to determine Heat Shock Protein-47 (HSP-47) for the characterization of primary skin fibroblast cells. Gold nanoparticle electrodes (GNPE) were modified with amino-functionalized graphene oxide layers and anti-HSP-47 were immobilized on this layer. The performance of the biosensor was tested with real samples as cell lysates. The low detection limit (LOD) and the linear detection limit (LOQ) of the biosensor were calculated as 9.47 pg/mL and 3.23 pg/mL, respectively. Linear measurement range was obtained between 10-160 pg/mL HSP-47. The reproducibility of the biosensor was good. | en_US |
| dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TuBTAK) [118S595] | en_US |
| dc.description.sponsorship | We are grateful to thank The Scientific and Technological Research Council of Turkey (TuBTAK) for supporting our TuBTAK-1005 project with the project number of 118S595. | en_US |
| dc.description.sponsorship | TÜBİTAK; Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (118S595); Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK | |
| dc.identifier.doi | 10.1002/elan.202060264 | |
| dc.identifier.issn | 1040-0397 | |
| dc.identifier.issn | 1521-4109 | |
| dc.identifier.scopus | 2-s2.0-85088816714 | |
| dc.identifier.uri | https://doi.org/10.1002/elan.202060264 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14365/778 | |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley-V C H Verlag Gmbh | en_US |
| dc.relation.ispartof | Electroanalysıs | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | HSP-47 | en_US |
| dc.subject | impedance | en_US |
| dc.subject | gold nanoparticle | en_US |
| dc.subject | graphene | en_US |
| dc.subject | chronoimpedance | en_US |
| dc.subject | Hsp47 | en_US |
| dc.title | Rapid Fibroblast Cell Culture Characterization With Impedimetric Label-Free Heat Shock Protein-47 Biosensor | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.id | Kocak, Ayse/0000-0002-1510-2937 | |
| gdc.author.id | uygun, zihni onur/0000-0001-9045-7271 | |
| gdc.author.id | demirdover, cenk/0000-0001-8387-5096 | |
| gdc.author.scopusid | 55935858800 | |
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| gdc.author.scopusid | 55936538800 | |
| gdc.author.scopusid | 6603568496 | |
| gdc.author.wosid | Kocak, Ayse/AAZ-7831-2020 | |
| gdc.author.wosid | uygun, zihni onur/P-8783-2015 | |
| gdc.author.wosid | demirdover, cenk/J-6211-2013 | |
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| gdc.description.department | İEÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü | en_US |
| gdc.description.departmenttemp | [Harmancı, Duygu; Sezgin, Ayse Kocak] Dokuz Eylul Univ, Grad Sch Hlth Sci, Mol Med Dept, Izmir, Turkey; [Uygun, Zihni Onur; Sagin, Ferhan Girgin] Ege Univ, Fac Med, Med Biochem Dept, Izmir, Turkey; [Demirdover, Cenk] Dokuz Eylul Univ, Sch Med, Plast Reconstruct & Aesthet Surg Dept, Izmir, Turkey; [Akdogan, Gul] Izmir Univ Econ, Sch Med, Med Biochem Dept, Izmir, Turkey | en_US |
| gdc.description.endpage | 2315 | en_US |
| gdc.description.issue | 10 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q2 | |
| gdc.description.startpage | 2310 | en_US |
| gdc.description.volume | 32 | en_US |
| gdc.description.wosquality | Q3 | |
| gdc.identifier.openalex | W3046281449 | |
| gdc.identifier.wos | WOS:000553463300001 | |
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| gdc.oaire.keywords | gold nanopArticle | |
| gdc.oaire.keywords | graphene | |
| gdc.oaire.keywords | impedance | |
| gdc.oaire.keywords | chronoimpedance | |
| gdc.oaire.keywords | gold nanoparticle | |
| gdc.oaire.keywords | HSP-47 | |
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| gdc.oaire.sciencefields | 0301 basic medicine | |
| gdc.oaire.sciencefields | 0303 health sciences | |
| gdc.oaire.sciencefields | 03 medical and health sciences | |
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| gdc.virtual.author | Akdoğan, Gül | |
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