PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection

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Citation - WoS: 2
Citation - Scopus: 1
Adjuvant Effects of Chemotherapeutics and Metformin on Mfe-319 Endometrial Carcinoma Cell Line
(Editura Acad Romane, 2020) Aydemir, Isil; Uluer, Elgin Turkoz; Korkmaz, Oya; Tuglu, Mehmet Ibrahim; Inan, Sevinc
We aimed to investigate the cytotoxicity of Metformin, Cisplatin, and Paclitaxel on MFE-319 endometrial carcinoma cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and immunocytochemistry assays. Half maximal inhibitory concentration (IC50) doses of three drugs alone and in the dual combinations were applied to the cells. Immunocytochemical method was performed for the cell survival and for phosphatidylinositol 3-kinase (PI3K), phosphorylated extracellular regulated kinases (pErk)-1/2, Akt-1, phosphorylated Akt (pAkt)-1/2/3 cell growth markers and angiogenic vascular endothelial growth factor (VEGF). Immunoreactivities were evaluated using H-score and analyzed using the one-way analysis of variance (ANOVA) test for statistics. It was found that these drugs caused a decrease in the immunoreactivities of these markers. Particularly, dual combination of Paclitaxel and Cisplatin decreased the immunoreactivities of PI3K, pErk-1/2, Akt-1, and pAkt-1/2/3. Cisplatin and Paclitaxel were more effective than Metformin; on the other hand, Metformin has been shown to enhance the efficacy of these two drugs. In vitro or in vivo further studies are needed to investigate the efficacy of these three drugs via PI3K/Akt signal pathway.
Citation - WoS: 2
Citation - Scopus: 2
Analysis of Nucleotide Changes in Rt-Pcr Primer/Probe Binding Regions in Sars-Cov Isolates Reported From Turkey
(Ankara Microbiology Soc, 2021) Demir, Ayse Banu; Bulgurcu, Alihan; Appak, Ozgur; Sayiner, Ayca Arzu
The SARS-CoV-2 virus, which caused the COVID-19 epidemic, caused more than 55 million cases and nearly 1.5 million deaths worldwide. For the microbiological diagnosis of the disease, the most valid method is detecting the presence of the viral genome by real-time reverse transcription polymerase chain reaction (rRT-PCR). However, due to the nature of the RNA viruses, frequent mutations may affect the sensitivity of the analyses made on the genetic material of the virus, such as PCR. In this study, we aimed to investigate the mutations in the primer-probe binding regions of the rRT-PCR panels used in COVID-19 diagnosis. SARS-CoV-2 whole genome sequence data (n= 194) isolated from COVID-19 cases in Turkey and uploaded on GISAID database from the centers in Istanbul (n= 78), Ankara (n= 58), Kars (n= 47), Bursa (n= 2), Adiyaman (n= 2), Erciyes (n= 1) and Kocaeli (n= 1) between March 17-September 14, 2020 were analyzed. In order to determine the nucleotide changes, SARS-CoV-2 sequences from Turkey were compared to the reference genome sequence (NC_045512.1) present in GenBank website. The constructed data set was aligned using the MAFFT program and was checked manually if the sequences were in the same frame by using the AliView program. Primer-probe binding sites of the thirteen SARS-CoV-2 rRT-PCR panels from seven different institutes (US CDC, China CDC, Charite CDC, Pasteur, HKU, Thailand, NIID) that are being used in COVID-19 diagnosis were evaluated in terms of nucleotide changes within the corresponding regions compared to the reference genome. Sequence diversities in the viral genomes were determined via positional nucleotide numerical calculator and entropy calculator modules and nucleotide and entropy changes in primer-probe binding regions for each rRT-PCR panel were examined. Among thirteen different primer-probe panels, nucleotide changes in the target regions of the seven primer-probe panels were determined. When viral sequences with nucleotide changes in the primer-probe binding regions were examined, the most common changes were observed in the China CDC N-forward primer and US CDC N3-forward primer binding regions. It is important that the kits to be used as diagnostic tests are designed specific to the regions with less nucleotide changes. Nucleotide changes may not be critical for DNA amplification for most PCR panels, but should be carefully monitored as they may affect the sensitivity of the assay. If the risk of alteration of the designed region is high, the primer - probe binding sites should be checked frequently and updated when necessary.
Citation - WoS: 1
Case Report-Right Atrial Mass: a Very Rare Presentation of Endometrial Cancer Metastasis
(Oxford Univ Press, 2023) Ertürk, Emre; Söyler, Onur; Pehlivan, Fatma Seher; Arslan, Çagatay
Background We report a case of a 47-year-old woman with right atrial metastasis of endometrioid adenocarcinoma, which is an uncommon clinical presentation for patients with endometrial cancer (EC). The principal aim of this case is to demonstrate the possibility of distant metastasis, something rarely encountered among this group of patients.Case summary Our patient, diagnosed with EC and receiving chemotherapy and radiotherapy after surgery, was found to have enhanced 18-fluorodeoxyglucose uptake inside the right atrium on the repeat positron emission tomography-computed tomography scan at the ninth month after initial diagnosis. Following trans-oesophageal echocardiography, cardiac magnetic resonance imaging showed a hyper-vascular mass with right atrial lateral wall involvement likely to be malignant in nature. A right atrial tumour was successfully removed by cardiovascular surgeons, and a pericardial patch was placed at the site of the excised atrium. The pathological examination showed EC metastasis. Following surgery, systemic treatment was planned for recurrent EC. The patient had an uneventful recovery after the surgery.Discussion Endometrial cancer is the most common gynaecologic malignancy and the fourth most common cancer in women. The lymphatic pathway is the main metastatic behaviour of EC; however, haematogenous metastases are not uncommon, especially in patients with higher stages of the disease. Our patient did not show any signs and symptoms of cardiac involvement. Nevertheless, clinicians should be alert for symptoms of cardiac involvement like new-onset murmur, embolism, or dyspnoea. Having known the behavioural pattern of the primary tumour, timely utilization of diagnostic imaging methods in accordance with clinical suspicions in patients with rapidly growing tumours can be lifesaving.
Citation - Scopus: 3
Clinical Impact of Hepatitis C Virus Genomic Variations
(Ankara Microbiology Society, 2015) Ergünay K.; Abacioglu H.
Hepatitis C virus (HCV) is a globally-dispersed agent of chronic hepatitis with a significant public health threat, affecting over 110 million individuals throughout the world. The increased risk for chronicity after exposure and the lack of a protective vaccine make HCV is a leading infectious cause of cirrhosis, liver failure requiring transplantation and hepatocellular carcinoma. The replicative process and infection dynamics in the host enable HCV to generate an array of closely-related but non-identical genetic variants known as quasispecies in the infected individuals. Pathogenesis and outcome in HCV infections are directly affected by the virus genetic heterogeneity, reflected as the emergence of quasispecies in infected individuals. The evolution of these highly-diverse viral populations in the host directly influences the disease course, via providing a pool of variants capable of resuming viral replication under extrinsic and/or intrinsic selective pressures. Viral quasispecies go through several alterations during the course of the infection, and provide a background for the selection of escape mutants from the host humoral and cell-mediated immune responses and antiviral treatment. Supported by the robust next generation sequencing techniques, recent studies have provided significant insights on the genomic diversity and progression as well as on the origin and the epidemiology of HCV. This review provides an overview of the mechanisms of HCV genetic variability, and the interactions with the host, that affects clinical disease, covering viral and host determinants of humoral and cell-mediated immune responses, alterations during the early and late stages of the infection and disease progression leading to chronicity. In addition, current findings in virus evolution and epidemiology were briefly interpreted from the inter-species and population perspectives. The impact of viral genomic heterogeneity on antiviral treatment in the era of direct-acting agents is also discussed, along with an overview of current methods employed for the characterization of viral diversity.
Clinical Management Strategies of Cardiologists in Heart Failure with Reduced Ejection Fraction in Türkiye
(Kare Publ, 2024) Türk, Uğur Önsel; Kocabaş, Umut; Özçalık, Emre; Kıvrak, Tarık; Altın, Cihan
Amaç: Kalp yetersizliği (KY) kılavuz önerilerinin klinik pratikte uygulanması zaman almaktadır ve genellikle gerçek yaşam şartlarında optimal düzeyde uygulanmamaktadır. Bu zorluğun önemli nedenlerinden biri hekim ile ilişkili faktörlerdir. Bu anket çalışmasının amacı, Türkiye’deki kardiyoloji uzmanlarının düşük ejeksiyon fraksiyonlu kalp yetersizliği (DEF-KY) tedavi tercihlerini belirlemektir. Yöntem: Anket çalışması 22 sorudan oluşmaktadır. Bu sorular, SurveyMonkey üzerinden yayınlanmış ve anket katılım linki birçok sosyal medya aracı üzerinden hekimlere ulaştırılmıştır. Bulgular: Anket çalışmasına Türkiye’de görev yapmakta olan 177 kardiyoloji uzmanı ve asistanı katıldı. Katılımcıların ortalama yaşı 39.5, %73.3’ü erkek, %38.7’si eğitim ve araştırma hastanelerinde görev yapmakta idi ve sadece %10.2’si kendisini KY uzmanı olarak tanımlamaktaydı. Katılımcıların %80.1’i, DEF-KY tanısı için sınır ejeksiyon fraksiyonu değerini %40 olarak kabul ettiklerini belirttiler. Hekimlerin %52.6’sı anjiyotensin reseptör-neprilisin inhibitörü (ARNi) tedavisini ‘‘en etkili KY tedavisi’’ olarak kabul ettiklerini belirtmelerine karşın, %62.7’si ilacın geri ödeme kısıtlılıkları ve fiyatı nedeniyle, DEF-KY tedavisine ARNi yerine anjiyotensin dönüştürücü enzim inhibitörü (ADEi) ile başlamak zorunda kaldıklarını belirttiler. Katılımcıların %52.3’ü tedaviye farklı bir KY ilaç sınıfını eklemenin, halihazırda kullanılan ilaçların dozunu arttırmaktan daha önemli olduğunu belirttiler. Hekimlerin %69.5’i yeni tanı KY hastalarında, hastane yatışı sırasında dört KY ilaç sınıfını aynı anda başlamanın mümkün olduğunu belirttiler. Buna karşın günlük pratiklerinde, sırasıyla ADEi/ARNI, beta–bloker, mineralokortikoid reseptör antagonisti ve sodyum-glukoz ko-transporter 2 inhibitörlerini başladıkları sıralı yaklaşımı daha çok tercih ettiklerini belirttiler. Sonuç: Bu anket çalışması, kılavuz önerileri ile gerçek yaşam uygulamaları arasında önemli farklılıklar olduğunu ortaya koymaktadır. Kılavuz önerilerinin, klinik pratikte uygulanabilmesi için sağlık sağlayıcıların gerekli önlemleri almalarına ve düzenlemeleri yapmalarına ihtiyaç vardır.
Citation - WoS: 3
Citation - Scopus: 3
A Combination of Heart Rate-Corrected Qt Interval and Grace Risk Score Better Predict Early Mortality in Patients With Non-St Segment Elevation Acute Coronary Syndrome
(Aves, 2022) Inci, Saadet Demirtas; Tekindal, Mustafa Agah; Altinsoy, Meltem; Ozbeyaz, Nail Burak; Sunman, Hamza; Tas, Alperen; Yilmaz, Sabiye
Objective: This study aimed to evaluate whether the addition of heart rate-corrected QT interval prolongation to the Global Registry of Acute Coronary Events risk score improves the predictive value for early mortality in patients with non-ST segment elevation acute coronary syndrome. Methods: We retrospectively screened our database for consecutive non-ST-segment elevation acute coronary syndrome patients between January 2017 and July 2019. The demographic and clinical parameters were acquired via chart review. All electrocardiograms were reviewed by 2 physicians. QT interval was measured using the tangent method. Early mortality was defined as all-cause death observed during the hospital stay or within 30 days after discharge. Results: The final study population consisted of 283 patients, there were 17 early deaths. Ten of 59 patients with prolonged corrected QT intervals died (16.9%, P < .001). Both the Global Registry of Acute Coronary Events risk score (odds ratio: 1.032; 95% CI: 1.012-1.053; P = .002) and corrected QT interval (odds ratio: 1.026; 95% CI: 1.007-1.045; P = 0.007) independently predicted early mortality. The area under value was 0.769 (95% CI: 0.674-0.863, P < .001) for the corrected QT interval and 0.780 (95% CI:0.681-0.878; P < .001) for the Global Registry of Acute Coronary Events risk score alone. However, when the corrected QT interval and the Global Registry of Acute Coronary Events risk score were combined, it was found to be 0.808 (95% CI: 0.713-0.904, P < .001). Conclusion: This study is the first to report that prolonged corrected QT and the Global Registry of Acute Coronary Events risk score independently predict early mortality and a combination of these 2 factors may improve the predictive value for early mortality in patients with ST-segment elevation acute coronary syndrome.
Citation - WoS: 3
Citation - Scopus: 3
Comparison of Single- and Double-Plate Fixation Techniques in the Treatment of Nonunions of the Humeral Shaft
(Atha Comunicacao & Editora, 2022) Akdemir, Mehmet; Biçen, Ahmet Çağdaş; Ozkan, Mustafa
Introduction: Humerus shaft fractures may be treated conservatively or surgically. In 2.5% to 13% of cases, nonunion is observed, and it leads to severe pain and morbidity. Plate osteosynthesis has become popular in the treatment of nonunion of the humeral shaft. In this study, we compared the clinical outcomes of patients with humerus shaft nonunion whom we treated with single- or double-plate fixation. Materials and Methods: Fifty-three patients diagnosed with aseptic humeral shaft nonunion and treated with plate fixation were included in the study. Patients were evaluated according to the number of plates (single vs. double plates). The two groups were subjected to statistical evaluation according to their clinical and radiographical results. Results: The average age of the patients was 53 years (range: 1-86); 28 (52.8%) were female and 25 (47.2%) were male. The union rate was 90.32% for single plate and 90.91% for double plate fixation. There was no statistically significant difference between single and double plates in the clinical and radiographical results (union time, union rate, Q-DASH score) (p > 0.05). There was a significant correlation between age and union times / Q-DASH scores according to the Spearman correlation test (p < 0.05). Transient radial nerve neuropraxia developed in 2 patients and 1 patient suffered from an infection that was treated with debridement and antibiotic therapy. Conclusion: In our study, similar good results were obtained with single and double plates. In treatment of humeral shaft nonunions, a second plate is not needed if enough stability is provided with single plate fixation.
Citation - WoS: 3
Citation - Scopus: 2
A Comparison of the Molecular Distribution of Proangiogenic Factors in Endometrium of Missed Abortions and of Voluntary First Trimester Termination Cases
(7847050 Canada Inc, 2015) Ozcakir, T.; Turan, M. A.; Simsek, F.; Atay, C.; Vatansevers, S.; Ozbilgin, K.
Objective: The authors aimed to evaluate the angiogenic changes that occur in the cases with missed abortions compared with the voluntary termination of pregnancy as control group, with this controlled clinical study. Materials and Methods: The study included fifteen healthy volunteer women with unwanted pregnancy less than 10th gestational week in an academic research environment. The patients were 19 women between 6th and 11th gestational weeks diagnosed with missed abortion as the patient group. Immunohistochemistry was utilized to examine temporal and spatial expression of vascular endothelial growth factor (VEGF) and their two receptors: VEGF-Rl (Flt-1) and VEGF-R2 (Flk-1/KDR), and Trombospondin-1, eNOS, iNOS, and HIF-1 alpha in the both deciduas and placenta of the both groups. Results: This study discovered the significant difference (p < 0.005) between the groups of controlled and missed abortion in the decidual and placental cell components, and has put forward that thrombospondin and iNOS have an impact on abortion through antiangiogenic effect in cases of missed abortions. Conclusions: The potential role of molecules affecting angiogenesis in the etiology of missed abortion has been evaluated and the authors aimed for this to be a guide for studies on further treatments and on the prevention of the development of missed abortions.
Consistent Estimation of Complete Neuronal Connectivity in Large Neuronal Populations Using Sparse Shotgun Neuronal Activity Sampling
(Springer, 2016) Mishchenko, Yuriy
We investigate the properties of recently proposed shotgun sampling approach for the common inputs problem in the functional estimation of neuronal connectivity. We study the asymptotic correctness, the speed of convergence, and the data size requirements of such an approach. We show that the shotgun approach can be expected to allow the inference of complete connectivity matrix in large neuronal populations under some rather general conditions. However, we find that the posterior error of the shotgun connectivity estimator grows quickly with the size of unobserved neuronal populations, the square of average connectivity strength, and the square of observation sparseness. This implies that the shotgun connectivity estimation will require significantly larger amounts of neuronal activity data whenever the number of neurons in observed neuronal populations remains small. We present a numerical approach for solving the shotgun estimation problem in general settings and use it to demonstrate the shotgun connectivity inference in the examples of simulated synfire and weakly coupled cortical neuronal networks.
Detection of Hypokalemia, Hyponatremia, and Hyperkalemia in Heart Failure Patients Using Artificial Intelligence Techniques via Electrocardiography
(2026) Iyigun, U.; Kerkutluoglu, M.; Güneş, H.; Kahramanoğullari, Faris; Kivrak, T.; Murat, B.; Küçükler, N.
OBJECTIVE: Detection and monitoring of electrolyte imbalances are essential for the appropriate treatment of many metabolic diseases. However, no reliable and noninvasive tool currently exists for such detection. Electrolyte disorders, particularly in heart failure patients, can lead to life-threatening situations, which may often develop as a result of medications used in routine treatment. METHOD: In this study, we developed a deep learning model (DLM) using electrocardiography (ECG) to detect electrolyte imbalances in heart failure patients and evaluated its performance in a multicenter setting. Seventeen different centers participated in this study. Heart failure patients (ejection fraction ≤ 45%) who had blood electrolyte measurements and ECG taken on the same day were included. Patients were divided into four groups: those with normal electrolyte values, those with hypokalemia, those with hyperkalemia, and those with hyponatremia. Patients who developed electrolyte disorders due to medications used for heart failure were classified in the relevant group. Confidence intervals (CI): We computed 95% CIs for area under the receiver operating characteristic curve (AUROC) via stratified bootstrap (2,000 resamples at the patient level) and 95% CIs for accuracy using the Wilson score interval for binomial proportions. RESULTS: The accuracy rates of the DLM in detecting hyponatremia, hypokalemia, and hyperkalemia were 83.33%, 95.33%, and 95.77%, respectively. CONCLUSION: The proposed DLM demonstrated high performance in detecting electrolyte imbalances. These results suggest that a DLM can be used to detect and monitor electrolyte imbalances using ECG on a daily basis.
The Effects of Late-Onset Depression on Brain Activity During an Episodic Memory Task
(Turkish Neuropsychiatry Assoc-Turk Noropsikiyatri Dernegi, 2025) Gulec, Zeynep Naz; Ercan, Melis; Erdogan, Yigit; Oguz, Kaya; Uyar, Aslihan; Burhanoglu, Birce Begum; Gonul, Ali Saffet
Introduction: Late-onset depression (LOD) has been implicated in irreversible cognitive decline, potentially mirroring early Alzheimer's Disease (AD) pathology. This study aimed to investigate brain activity differences during an episodic memory (EM) task in LOD patients compared to healthy controls (HC). Methods: We recruited 15 LOD patients and 13 HC matched for age and gender. Participants completed a face-name association task during functional magnetic resonance imaging (fMRI) focusing on both the encoding and retrieval phases of EM. Results: The statistical contrast between the groups revealed that the HC group showed increased activity in the left visual association cortex (VAC) and left caudate compared to the LOD group during the encoding task. During the face recognition task, the HC group showed increased activity in the right caudate, and during the name recognition task, they showed increased activity in the right frontal eye field (FEF) compared to the LOD group. Conclusion: The differences observed between the HC and LOD groups in the VAC, caudate, and FEF suggest early changes in maintaining attention, goal-directed learning, EM formation, and coordination of information from storage to retrieval before apparent impairment develops in LOD. Although we did not find statistically significant activations in areas linked to increased vulnerability to AD, our findings of hypoactivation regions responsible for visual processing and attentional orienting in LOD patients are consistentwith hypoactivation patterns observed in AD patients in previous research. These results enhance our understanding of the neural mechanisms underlying memory impairments in LOD and their potential overlap with AD pathology.
Evaluation of Alternative Clinical Samples for the Detection of SARS-CoV and Influenza Virus by Automated Multiplex RT-PCR
(J Infection Developing Countries, 2025) Ozarslan, Muhammed A.; Parkan, Omur M.; Soylu, Mehmet; Acet, Oguzhan; Gokahmetoglu, Selma; Yuce, Zeynep Ture; Erensoy, Selda
Introduction: The aim of this study was to compare the performance of different clinical specimens-nasopharyngeal (NP) swabs collected by healthcare professionals (HCP-NP), self-collected nasal swabs (Sc-N), and saliva samples (S)-in diagnostic tests for investigating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and influenza A/B RNA. Methodology: These clinical samples were collected from 404 symptomatic cases and tested with the SARS-CoV-2 and influenza A/B RNA tests on the cobas 6800 System of Roche Molecular Systems (Roche Molecular Systems, Pleasanton, USA). The SARS-CoV-2 or influenza virus infection status was determined for all patients based on the predefined criteria and corresponding algorithms. Positive and negative predictive values (PPV, NPV), sensitivity, specificity, coefficient of variation (CV), interrater reliability, correlation, ,and days of sample collection of these three sample types were analyzed. Results: There was almost perfect agreement between the these sample types for the diagnosis of SARS-CoV-2 and influenza A. The overall performance (PPV, NPV, sensitivity) and reproducibility (CV <= 6%) were favorable. Additionally, they showed similar trends for days of sample collection. Conclusions: Diagnostic detection of SARS-CoV-2 and influenza RNA from Sc-N and S samples was comparable to HCP-NP samples. Using these samples would provide an advantage in diagnosing SARS-CoV-2 and influenza A infection, as they can be easily collected without the need for viral transport media.
Citation - WoS: 8
Citation - Scopus: 7
Evaluation of Demographic and Clinical Characteristics of Female Patients Presenting With Minoca and Differences Between Male Patients: a Subgroup Analysis of Minoca-Tr Registry
(Kare Publ, 2022) Gok, Gulay; Coner, Ali; Cinar, Tufan; Kilic, Salih; Yenercag, Mustafa; Oz, Ahmet; Ekmekci, Cenk
Objective: Although the prevalence and rate of myocardial infarction with non-obstructive coronary arteries (MINOCA) are higher in women than in men in previous cohorts, potential demographic and clinical differences between women who are diagnosed with MINOCA versus myocardial infarction with obstructive coronary arteries (MIOCA) have not been studied till date. In this study, we aimed to document these characteristics and to compare them between female patients with MINOCA and MIOCA. Methods: The study was a subgroup analysis of the MINO-CA-TR study. The study was a multi-center, observational cohort study that was conducted in Turkey between March 2018 and October 2018. In this study, 477 (29.3%) female patients who had been diagnosed with acute myocardial infarction were evaluated. Results: Of these women, 49 (10.3%) were diagnosed with MINOCA (mean age 58.9 +/- 12.9 years) and 428 (89.7%) had a final diagnosis of MIOCA (mean age 67.4 +/- 11.8 years). The prevalence of hypertension, hyperlipidemia, and diabetes mellitus was significantly lower in the MINOCA group than in the MIOCA group. In addition, the MINOCA group had higher rates of recent flu history and non-ST elevation myocardial infarction (NSTEMI) presentation than the MIOCA group. There were significant clinical differences in patients with MINOCA in terms of sex. The female patients were older, had higher systolic blood pressures, and lower hemoglobin levels than male patients. Conclusion: The study revealed that the prevalence of traditional coronary artery disease risk factors was lower in female patients with MINOCA than in those who had final diagnosis of MIOCA.
Citation - WoS: 1
Citation - Scopus: 2
Evaluation of Treatment Safety in Patients With Atrial Fibrillation on Edoxaban Therapy in Real-Life in Turkey Study: Design and Rationale
(Aves, 2022) Türk Uğur Önsel; Alioglu, Emin; Mavioglu, Zafer; Diker, Erdem; Ozpelit, Ebru; De Caterina, Raffaele
Objective: Safety and effectiveness of edoxaban was demonstrated in phase III, Effective Anticoagulation with Factor Xa Next Generation in Atrial Fibrillation-Thrombolysis in Myocardial Infarction 48 (ENGAGE-AF-TIMI 48) trial and is being confirmed in the post-authorization Edoxaban Treatment in routiNe clinical prActice for patients with Atrial Fibrillation in Europe (ETNA-AF-Europe) study in patients with atrial fibrillation. However. any post-authorization safety study focusing on the safety of edoxaban treatment in Turkey with a prospective design has not been performed yet. The Evaluation of Treatment Safety in Patients with Atrial Fibrillation on Edoxaban Therapy in Real-Life in Turkey (ETAF-TR) study is designed to evaluate the safety and effectiveness of edoxaban treatment in atrial fibrillation in routine practice. The present article describes the design and rationale for the ETAF-TR Study. Methods: The ETAF-TR (NCT04594915) is a national. multicenter. prospective, observational study that enrolled 858 patients from 32 centers. The primary outcome of the ETAF-TR study is any overt bleeding (consisting of major bleeding or clinically relevant nonmajor bleeding or any bleeding that does not meet this definition but is considered as overt bleeding by the participating physician). Effectiveness, treatment persistence. and posology will also be evaluated in an explorative manner. The overall duration of follow-up will be 1 year: the first patient was enrolled in August 2020. Conclusions: Results of ETAF-TR wilt add data from clinical practice to those from ENGAGE-AF trial and also ETNA-AF study. Comparing their results will enable to test the external validity of ENGAGE-AF trial in the country conditions.
Citation - WoS: 8
Citation - Scopus: 7
The Expression of Forkhead Transcription Factors in Decidua and Placenta in Patients With Missed Abortion
(I R O G Canada, Inc, 2015) Ozbilgin, K.; Kahraman, B.; Turan, A.; Atay, C.; Vatansever, S.; Inan, S.; Ozcakir, T.
Background: Forkhead transcription factors 3a (FOXO3a) has pleiotropic biological functions in the female reproductive tract. FOXO3a has a function in decidualization, in placental development, and also in inhibition of apoptosis. This study aims to investigate a possible role of FOXO3a in missed abortion. Materials and Methods: Decidual and placental tissue samples were obtained from the women with unwanted pregnancy as the control group and with missed abortion as the patient group. Immunohistochemistry technique was utilized to compare FOXO3a expression of the decidual cells in uterine decidual stroma and cytotrophoblast-syncytiotrophoblast cells in placental villous stroma. Immunohistochemistry was evaluated semi-quantitatively utilizing the H-score technique. Results: It was demonstrated that H-Scores of FOXO3a expression in both uterine decidual stroma were increased in the missed abortion group (255.83 +/- 12.41) than in the normal pregnancy group (133.33 +/- 17.43). It was also shown that there was no difference between non-decidual area of the endometrium of the normal pregnancy and the missed abortion group (30.33 +/- 4.32; 39.66 +/- 14.30, respectively) and placental villous stroma (13.00 +/- 1.89; 13.00 +/- 1.67, respectively). However, the immunoreactivity of cytotrophoblast and syncytiotrophoblast cells significantly increased in the missed abortion group (18.83 +/- 1.47; 322.00 +/- 6.06, respectively) than in the normal pregnancy group (11.00 +/- 1.26; 254.00 +/- 8.17, respectively) (p < 0.05). Conclusion: These data support the hypothesis that increased FOXO3a expression in missed abortion may prevent the discharge of dead fetus to maintain decidualization, prevention of oxidative stress, immunomodulation, and inhibition of apoptosis.
Fungal Sinusitis Due To Mucormycosis in a Diabetic Immunosuppressed Patient With Acute Myeloid Leukemia (AML)
(J infection Developing Countries, 2024) Ece, Gulfem; Ciger, Ejder; Acar, Celal; Cagirgan, Seckin; Cetin, Ogulcan
Introduction: Mucormycosis is an acute onset, invasive, fungal infection, characterized by organ involvement, and caused by Mucor, Rhizopus, or Absidia. Our aim was to present a case of mucormycotic infection and emphasize its importance in a diabetic immunosuppressed patient with acute myeloid leukemia (AML). Case presentation: A 68-year-old hypertensive and diabetic male patient with a diagnosis of AML developed respiratory failure and exhibited diffuse bilateral consolidation in high-resolution computed tomography (HRCT). The treatment plan involved chemotherapy with cytarabine (200 mg/m2/day for 7 days) and daunorubicin (60 mg/m2/day for 3 days) starting on 20 July 2022. Posaconazole prophylactic treatment was initiated on 23 July 2022, to prevent fungal infections. Five days later there was a black necrotic appearance on the left wing of the nose. The patient underwent excision of the left wing of the nose. Mucor was detected in the excision tissue both histopathologically and in culture. A culture under lactophenol cotton blue (LFCB) staining displaying hyphal structures of Mucor was obtained. The patient died of progressive pneumonia and sepsis. Conclusions: Mucormycosis is an infection with high mortality, and should be considered in the early stages of diagnosis when dealing with immunosuppression patients.
Fungal Sinusitis Due To Mucormycosis in a Diabetic Immunosuppressed Patient With Acute Myeloid Leukemia (aml)
(Journal of Infection in Developing Countries, 2024) Ece, G.; Ciger, E.; Acar, C.; Cagirgan, S.; Cetin, O.
Introduction: Mucormycosis is an acute onset, invasive, fungal infection, characterized by organ involvement, and caused by Mucor, Rhizopus, or Absidia. Our aim was to present a case of mucormycotic infection and emphasize its importance in a diabetic immunosuppressed patient with acute myeloid leukemia (AML). Case presentation: A 68-year-old hypertensive and diabetic male patient with a diagnosis of AML developed respiratory failure and exhibited diffuse bilateral consolidation in high-resolution computed tomography (HRCT). The treatment plan involved chemotherapy with cytarabine (200 mg/m2/day for 7 days) and daunorubicin (60 mg/m2/day for 3 days) starting on 20 July 2022. Posaconazole prophylactic treatment was initiated on 23 July 2022, to prevent fungal infections. Five days later there was a black necrotic appearance on the left wing of the nose. The patient underwent excision of the left wing of the nose. Mucor was detected in the excision tissue both histopathologically and in culture. A culture under lactophenol cotton blue (LFCB) staining displaying hyphal structures of Mucor was obtained. The patient died of progressive pneumonia and sepsis. Conclusions: Mucormycosis is an infection with high mortality, and should be considered in the early stages of diagnosis when dealing with immunosuppression patients. Copyright © 2024 Ece et al.
Citation - WoS: 4
Citation - Scopus: 5
Identification of Differentially Expressed Genomic Repeats in Primary Hepatocellular Carcinoma and Their Potential Links To Biological Processes and Survival
(Scientific And Technological Research Council Turkey, 2021) Karakulah, Gokhan; Yandim, Cihangir
Hepatocellular carcinoma (HCC) is one of the deadliest cancers. Research on HCC so far primarily focused on genes and provided limited information on genomic repeats, which constitute more than half of the human genome and contribute to genomic stability. In line with this, repeat dysregulation was significantly shown to be pathological in various cancers and other diseases. In this study, we aimed to determine the full repeat expression profile of HCC for the first time. We utilised two independent RNA-seq datasets obtained from primary HCC tumours with matched normal tissues of 20 and 17 HCC patients, respectively. We quantified repeat expressions and analysed their differential expression. We also identified repeats that are cooperatively expressed with genes by constructing a gene coexpression network. Our results indicated that HCC tumours in both datasets harbour 24 differentially expressed repeats and even more elements were coexpressed with genes involved in various metabolic pathways. We discovered that two L1 elements (L1M3b, L1M3de) were downregulated and a handful of HERV subfamily repeats (HERV-Fc1-int, HERV3-int, HERVE_a-int, HERVK11D-int, HERVK14C-int, HERVL18-int) were upregulated with the exception of HERV1_LTRc, which was downregulated. Various LTR elements (LTR32, LTR9, LTR4, LTR52-int, LTR70) and MER elements (MER11C, MER11D, MER57C1, MER9a1, MER74C) were implicated along with few other subtypes including Charlie12, MLT2A2, Tigger15a, Tigger 17b. The only satellite repeat differentially expressed in both datasets was GSATII, whose expression was upregulated in 33 (>90%) out of 37 patients. Notably, GSATII expression correlated with HCC survival genes. Elements discovered here promise future studies to be considered for biomarker and HCC therapy research. The coexpression patterns GSATII satellite with HCC survival genes and the fact that it has been upregulated in the vast majority of patients make this repeat particularly stand out for HCC.
Citation - WoS: 15
Citation - Scopus: 17
Identification of the Nucleotide Substitutions in 62 Sars-Cov Sequences From Turkey
(Tubitak Scientific & Technical Research Council Turkey, 2020) Demir, Ayse Banu; Benvenuto, Domenico; Abacioglu, Hakan; Angeletti, Silvia; Ciccozzi, Massimo
A previously unknown coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been shown to cause coronavirus disease 2019 (COVID-19) pandemic. The first case of COVID-19 in Turkey has been declared in March 11th, 2020 and from there on, more than 150,000 people in the country have been diagnosed with the disease. In this study, 62 viral sequences from Turkey, which have been uploaded to GISAID database, were analyzed by means of their nucleotide substitutions in comparison to the reference SARS-CoV-2 genome from Wuhan. Our results indicate that the viral isolates from Turkey harbor some common mutations with the viral strains from Europe, Oceania, North America and Asia. When the mutations were evaluated, C3037T, C14408T and A23403G were found to be the most common nucleotide substitutions among the viral isolates in Turkey, which are mostly seen as linked mutations and are part of a haplotype observed high in Europe.
İnfluenza A/B Virüs ve RSV Validasyon Standartlarının Dijital PCR ile Kantitasyonu
(Ankara Microbiology Soc, 2025) Sayıner, Ayca Arzu; Bulgurcu, Alıhan
Mikrobiyolojik tanı laboratuvarlarında kullanılacak tanı testleri için kantitatif standartların kullanıldığı yöntem doğrulama (verification) veya geçerli kılma (validation) çalışmaları gereklidir. Nükleik asit testlerinde sentetik nükleik asit veya plazmit yerine tam virüs içeren standartların kullanılması; ekstraksiyon, revers transkripsiyon ve amplifikasyonu içerecek şekilde tanı testinin tüm basamaklarının gerçek yaşam koşullarında değerlendirilmesini sağlar. Solunum yolu virüsleri için nükleik asit testlerine yönelik ticari kantitatif standart materyaller sınırlıdır. Bu çalışmada; influenza A virüs (infA), influenza B virüs (infB) ve respiratuvar sinsityal virüs (RSV) için dijital polimeraz zincir reaksiyonu [digital polymerase chain reaction (dPCR)] kullanılarak, kantitatif nükleik asit standartları geliştirilmesi amaçlanmıştır. Çalışmada; RSV, infA, infB RNA pozitif olduğu bilinen nazofarengeal sürüntü örneklerinin havuzlanmasıyla hazırlanan örneklerdeki viral nükleik asit miktarı, ticari primer/prob setleri (Qiagen, Almanya) kullanılarak dPCR (QIAcuity, Qiagen) yöntemiyle belirlenmiştir. Nükleik asit ekstraksiyonu, ticari bir kit (Xi’an Tianlong Science&Technology Co, Çin) kullanılarak yapılmıştır. dPCR yönteminin infA, infB ve RSV için analitik duyarlılık (LoD) ve kantitasyon alt sınırı (LoQ), çalışma içi ve çalışmalar arası tekrarlanabilirliği ve doğrusallığı belirlenmiştir. dPCR ile çalışılan örnekler, kantitatif revers transkripsiyon gerçek zamanlı [quantitative reverse transcription realtime (qrRT)] PCR (qRT-PCR) ile de çalışılarak Ct değerleri belirlenmiştir. Ct değerleri ile dPCR-kantitasyon sonuçları arasındaki ilişki lineer regresyon ile değerlendirilmiştir. İstatistiksel analiz GraphPad Prism 10.4.0 (GraphPad, ABD) ve Excel Analysis ToolPak kullanılarak yapılmıştır. İnfA, infB ve RSV için dPCR yönteminin LoD değerleri sırasıyla 93.75, 15.59 ve 26.23 kopya/mL olarak belirlenmiştir. dPCR yönteminin çalışma içi tekrarlanabilirliği (varyasyon katsayısı, %CV), düşük viral yükü olan örneklerde daha yüksek olmak üzere 0.06-7.97 arası saptanmıştır. Çalışmalar arası tekrarlanabilirlik 0.73-5.41 olarak bulunmuştur. İnfA ve infB için 3-4 log10, RSV için 7 log10 aralığında dilüsyonlar ile yapılan doğrusallık analizinde her üç virüs için de r 2≥ 0.99 olarak bulunmuştur. dPCR ile ölçülen konsantrasyonların, qRT-PCR Ct sonuçları ile korele olduğu saptanmıştır. dPCR ile qRT-PCR testlerinin çalışma içi ve çalışmalar arası tekrarlanabilirlik sonuçları karşılaştırıldığında, dPCR’nin %CV değerinin anlamlı olarak daha düşük olduğu saptanmıştır (p= 0.0312). Çalışma sonuçları dPCR yönteminin, kantitatif nükleik asit standartları elde etmede tekrarlanabilirliği yüksek ve güvenilir bir yöntem olduğunu göstermiştir. Elde edilen kantitatif standartlar ile viral yük belirlemeye yönelik tanı yöntemleri geliştirmek ve/veya bu tür testlerin yöntem onayı analizlerini yapmak mümkündür. Sonuç olarak çalışmada, havuzlanmış hasta örnekleri kullanılarak dPCR yöntemiyle infA, infB ve RSV için güvenilir kantitatif nükleik asit standartlar elde edilmiş ve dPCR yönteminin performans analizleri gerçekleştirilmiştir. Bu çalışma, dPCR ile kantitatif viral nükleik asit standartlarının üretimine bir örnek olmuştur.

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