TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14365/4
Browse
15 results
Search Results
Article A Preliminary Study of Possible Fibrotic Role of Meprin Metalloproteases in Scleroderma Patients(Turkish League Against Rheumatism (TLAR), 2021-12-31) Avşar, Aydan Köken; Merih Birlik, A.; Koçak, Ayşe; Harmancı, Duygu; Akdoğan, Gül; Birlik, Merih; Birlik, A. Merih; Güner, Gül AkdoganObjectives: This study aims to investigate the possible fibrotic role of meprin metalloproteases and possible fibrotic effects of activator protein-1 (AP-1) in scleroderma patients. Patients and methods: Between April 2018 and April 2019, a total of 85 scleroderma patients (9 males, 76 females; mean age: 54.9 +/- 12.1 years; range, 22 to 80 years) who met the 2013 American College of Rheumatology/European League Against Rheumatism criteria and 80 healthy control individuals (10 males, 70 females; mean age 42.9 +/- 10.2 years; range, 19 to 65 years) were included. Patients' data and blood samples were collected. Messenger ribonucleic acid expressions of interleukin (IL)-6, AP-1 subunits, and tumor necrosis factor-alpha (TNF-alpha) were analyzed by quantitative real-time polymerase chain reaction. Serum meprin alpha and beta protein levels were analyzed using the enzyme-linked immunosorbent assay. Results: Meprin alpha and meprin beta protein levels increased in scleroderma patients. The AP-1 subunits (c-Fos, c-Jun), IL-6, and TNF-alpha increased in scleroderma patients, compared to controls. Conclusion: Our results provide evidence showing that increased meprins levels may be related to AP-1 levels and increased meprins levels may responsible for increased inflammatory TNF-alpha and IL-6 levels. All these data suggest meprins as promising therapeutic targets to restore the balance between inflammation and extracellular matrix deposition in scleroderma.Article İnfluenza A/B Virüs ve RSV Validasyon Standartlarının Dijital PCR ile Kantitasyonu(Ankara Microbiology Soc, 2025-07-26) Sayıner, Ayca Arzu; Bulgurcu, AlıhanMikrobiyolojik tanı laboratuvarlarında kullanılacak tanı testleri için kantitatif standartların kullanıldığı yöntem doğrulama (verification) veya geçerli kılma (validation) çalışmaları gereklidir. Nükleik asit testlerinde sentetik nükleik asit veya plazmit yerine tam virüs içeren standartların kullanılması; ekstraksiyon, revers transkripsiyon ve amplifikasyonu içerecek şekilde tanı testinin tüm basamaklarının gerçek yaşam koşullarında değerlendirilmesini sağlar. Solunum yolu virüsleri için nükleik asit testlerine yönelik ticari kantitatif standart materyaller sınırlıdır. Bu çalışmada; influenza A virüs (infA), influenza B virüs (infB) ve respiratuvar sinsityal virüs (RSV) için dijital polimeraz zincir reaksiyonu [digital polymerase chain reaction (dPCR)] kullanılarak, kantitatif nükleik asit standartları geliştirilmesi amaçlanmıştır. Çalışmada; RSV, infA, infB RNA pozitif olduğu bilinen nazofarengeal sürüntü örneklerinin havuzlanmasıyla hazırlanan örneklerdeki viral nükleik asit miktarı, ticari primer/prob setleri (Qiagen, Almanya) kullanılarak dPCR (QIAcuity, Qiagen) yöntemiyle belirlenmiştir. Nükleik asit ekstraksiyonu, ticari bir kit (Xi’an Tianlong Science&Technology Co, Çin) kullanılarak yapılmıştır. dPCR yönteminin infA, infB ve RSV için analitik duyarlılık (LoD) ve kantitasyon alt sınırı (LoQ), çalışma içi ve çalışmalar arası tekrarlanabilirliği ve doğrusallığı belirlenmiştir. dPCR ile çalışılan örnekler, kantitatif revers transkripsiyon gerçek zamanlı [quantitative reverse transcription realtime (qrRT)] PCR (qRT-PCR) ile de çalışılarak Ct değerleri belirlenmiştir. Ct değerleri ile dPCR-kantitasyon sonuçları arasındaki ilişki lineer regresyon ile değerlendirilmiştir. İstatistiksel analiz GraphPad Prism 10.4.0 (GraphPad, ABD) ve Excel Analysis ToolPak kullanılarak yapılmıştır. İnfA, infB ve RSV için dPCR yönteminin LoD değerleri sırasıyla 93.75, 15.59 ve 26.23 kopya/mL olarak belirlenmiştir. dPCR yönteminin çalışma içi tekrarlanabilirliği (varyasyon katsayısı, %CV), düşük viral yükü olan örneklerde daha yüksek olmak üzere 0.06-7.97 arası saptanmıştır. Çalışmalar arası tekrarlanabilirlik 0.73-5.41 olarak bulunmuştur. İnfA ve infB için 3-4 log10, RSV için 7 log10 aralığında dilüsyonlar ile yapılan doğrusallık analizinde her üç virüs için de r 2≥ 0.99 olarak bulunmuştur. dPCR ile ölçülen konsantrasyonların, qRT-PCR Ct sonuçları ile korele olduğu saptanmıştır. dPCR ile qRT-PCR testlerinin çalışma içi ve çalışmalar arası tekrarlanabilirlik sonuçları karşılaştırıldığında, dPCR’nin %CV değerinin anlamlı olarak daha düşük olduğu saptanmıştır (p= 0.0312). Çalışma sonuçları dPCR yönteminin, kantitatif nükleik asit standartları elde etmede tekrarlanabilirliği yüksek ve güvenilir bir yöntem olduğunu göstermiştir. Elde edilen kantitatif standartlar ile viral yük belirlemeye yönelik tanı yöntemleri geliştirmek ve/veya bu tür testlerin yöntem onayı analizlerini yapmak mümkündür. Sonuç olarak çalışmada, havuzlanmış hasta örnekleri kullanılarak dPCR yöntemiyle infA, infB ve RSV için güvenilir kantitatif nükleik asit standartlar elde edilmiş ve dPCR yönteminin performans analizleri gerçekleştirilmiştir. Bu çalışma, dPCR ile kantitatif viral nükleik asit standartlarının üretimine bir örnek olmuştur.Article Expressions of the Satellite Repeat Hsat5 and Transposable Elements Are Implicated in Disease Progression and Survival in Glioma(Tubitak Scientific & Technological Research Council Turkey, 2024-08-23) Köse, Sıla Naz; Yaraş, Tutku; Bursalı, Ahmet; Oktay, Yavuz; Yandım, Cihangir; Karakulah, GökhanThe glioma genome encompasses a complex array of dysregulatory events, presenting a formidable challenge in managing this devastating disease. Despite the widespread distribution of repeat and transposable elements across the human genome, their involvement in glioma's molecular pathology and patient survival remains largely unexplored. In this study, we aimed to characterize the links between the expressions of repeat/transposable elements with disease progression and survival in glioma patients. Hence, we analyzed the expression levels of satellite repeats and transposons along with genes in low-grade glioma (LGG) and high-grade glioma (HGG). Endogenous transposable elements LTR5 and HERV_a-int exhibited higher expression in HGG patients, along with immune response-related genes. Altogether, 16 transposable elements were associated with slower progression of disease in LGG patients. Conversely, 22 transposons and the HSAT5 satellite repeat were linked to a shorter event-free survival in HGG patients. Intriguingly, our weighted gene coexpression network analysis (WGCNA) disclosed that the HSAT5 satellite repeat resided in the same module network with genes implicated in chromosome segregation and nuclear division; potentially hinting at its contribution to disease pathogenesis. Collectively, we report for the first time that repeat and/or transposon expression could be related to disease progression and survival in glioma. The expressions of these elements seem to exert a protective effect during LGG-to-HGG progression, whereas they could have a detrimental impact once HGG is established. The results presented herein could serve as a foundation for further experimental work aimed at elucidating the molecular regulation of glioma genome.Letter Citation - WoS: 1Citation - Scopus: 1About The Article Titled “a Different Scintigraphic Perspective On The Systolic Function Of The Left Ventricle-1” [“sol Ventrikül Sistolik Fonksiyonuna Sintigrafik Olarak Farklı Bir Bakış Açısı-1” Başlıklı Makale Hakkında](Galenos Publishing House, 2024-02-23) Taşçı, Cengiz; Tascs, Cengiz[No abstract available]Article Citation - WoS: 7Citation - Scopus: 7Effectiveness of a Program To Raise Awareness About Pneumococcal Vaccination Among Physicians and Patients With Chronic Respiratory Diseases: a Multicenter Cohort Study(AVES, 2024-08-27) Karakurt, Z.; Yalnız, E.; Altın, S.; Oruç, Ö.; Uslu, Ö.; Veske, N.Ş.; Kılınç, O.; Sayıner, Abdullah; Kul, Seval; Şimşek, NurdanOBJECTIVE: There is a need to increase patient and clinician awareness on the effectiveness of pneumococcal vaccination in at-risk groups. The aim of the study was to evaluate the effect of reminders for physicians and patients using the vaccination tracking system created in the hospital information management system (HIMS) on the vaccination rate, and the effect of pneumococcal vaccination on pneumonia-related hospitalization and mortality over a 12-month period. MATERIAL AND METHODS: This prospective observational cohort study was undertaken during a 2-year period in 3 tertiary care cen-ters. Patients were followed up for 12 months following vaccination, and hospital admissions and mortality were recorded via HIMS. During the campaign, HIMS transmitted pneumococcal immunization reminder messages in accordance with guideline recommendations to physicians’ computers and patients’ mobile phones. Educational posters on pneumococcal vaccination were posted in outpatient clinics. Regular seminars on the evidence for pneumococcal vaccination were organized. All patients who were hospitalized during the follow-up period for chronic obstructive pulmonary disease (COPD), asthma, lung cancer, or pneumonia were analyzed in relation to their vaccination history regarding clinical outcomes. RESULTS: A total of 29 530 patients were included in the study. During the study period, the annual vaccination rate increased by 74.4% and reached 4.8% in 3 hospitals (P = .001). The rates were 3.9% in patients older than 65 years without comorbidities and 5.2% in those with COPD and asthma (P = .002). In pneumococcal vaccine recipients, pneumonia-related hospital mortality was lower (relative risk (RR) = 0.19, CI 0.09-0.35, P < .001). CONCLUSION: It is possible to raise the rate of pneumococcal vaccination through awareness campaigns. Individuals with COPD and asthma are more willing to receive pneumococcal vaccination. Among patients hospitalized for pneumonia, prior pneumococcal vaccination is associated with lower mortality. © Author(s).Article Citation - WoS: 2Citation - Scopus: 2Adrenomedullin Has No Effect on Segmental Bone Defect Healing but Increases Bone Mineral Density in Rat Model(AVES, 2023-10-11) Kaymakoğlu, Mehmet; Ciftci, E.; Korkusuz, P.; Ozdemir, E.; Erden, M.E.; Turhan, E.Objective: This study aimed to investigate the effect of adrenomedullin on the healing of the segmental bone defect in a rat model. Methods: Thirty-six Wistar rats were randomly divided into 6 groups based on follow-up periods and administered a dose of adreno-medullin hormone. In each group, bilaterally, a 2-mm bone defect was created at the diaphysis of the radius. Sodium chloride solution was administered to sham groups 3 times a week for 4 and 8 weeks intraperitoneally. Adrenomedullin was administered to the study groups 3 times a week: 15 μg—4 weeks, 15 μg—8 weeks, 30 μg—4 weeks, and 30 μg—8 weeks, respectively. After euthanasia, the segmental defects were evaluated by histomorphometric [new bone area (NBA)] and microtomographic [bone volume (BV), bone surface (BS), and bone mineral density (BMD)] analyses. Results: Although the 4-and 8-week 15 μg administered study groups had higher NBA values than the other study and control groups, the histomorphometric analysis did not reveal any statistical difference between the control and study groups regarding NBA (P >.05). In microtomographic analysis, BV was higher in the 15 μg 4-week group than 30 μg 4-week group (296.9 vs. 208.5, P =.003), and BS was lower in the 30 μg 4-week group than in the 4-week control group (695.5 vs. 1334.7, P =.005), but overall, no significant difference was found between the control and study groups (P >.05). Despite these minor differences in histomorphometric and microtomographic criteria indicating new bone formation, the BMD values of the 15 μg 8-week study group showed a significant increase compared with the control group (P =.001, respectively). Conclusion: Adrenomedullin positively affected BMD at 15 μg, but this study could not show healing in the segmental defect site at different dose regimens. Further studies are needed to assess its effects on bone tissue trauma. © 2023, AVES. All rights reserved.Article Citation - WoS: 5Citation - Scopus: 5The Effects of Playing Digital Games on Children's Pain, Fear, and Anxiety Levels During Suturing: a Randomized Controlled Study(Wolters Kluwer Medknow Publications, 2023-07) Kavlakcı, Muhammet; Öğce, Filiz; Yavan, TulayOBJECTIVE: The aim was to determine the effects of digital game play on children's pain, fear, and anxiety levels during suturing. METHODS: Data were obtained from 84 children between the ages of 8 and 17 years at the pediatric emergency department between January 16 and March 19, 2020, using the Socio-Demographic and Clinical Characteristics Form, the Wong-Baker Faces Pain Rating Scale (WBFPS), the Visual Analogue Scale (VAS), the Fear of Medical Procedures Scale (FMPS), and the State-Trait Anxiety Inventory for Children (STAI-CH). A four-block randomization system was used. The study group (n = 42) played digital games during the suturing procedure, unlike the control group (n = 42). Ethical permissions were obtained from the ethical committee, hospital, and families. RESULTS: Before the suturing procedure, there was no statistically significant difference between the groups' mean scores. The intervention group was found to have statistically significantly lower WBFPS and VAS pain scores than the control group during the suturing procedure, and after the procedure, statistically significantly lower WBFPS, VAS, FMPS, and STAI-CH mean scores than the control group. CONCLUSIONS: The digital game-playing approach applied before and during the suture procedure was found to be effective in reducing children's pain, fear, and anxiety levels.Article Citation - Scopus: 3Clinical Impact of Hepatitis C Virus Genomic Variations(Ankara Microbiology Society, 2015-10-08) Ergünay K.; Abacioglu H.; Ergünay, Koray; Abacioglu, HakanHepatitis C virus (HCV) is a globally-dispersed agent of chronic hepatitis with a significant public health threat, affecting over 110 million individuals throughout the world. The increased risk for chronicity after exposure and the lack of a protective vaccine make HCV is a leading infectious cause of cirrhosis, liver failure requiring transplantation and hepatocellular carcinoma. The replicative process and infection dynamics in the host enable HCV to generate an array of closely-related but non-identical genetic variants known as quasispecies in the infected individuals. Pathogenesis and outcome in HCV infections are directly affected by the virus genetic heterogeneity, reflected as the emergence of quasispecies in infected individuals. The evolution of these highly-diverse viral populations in the host directly influences the disease course, via providing a pool of variants capable of resuming viral replication under extrinsic and/or intrinsic selective pressures. Viral quasispecies go through several alterations during the course of the infection, and provide a background for the selection of escape mutants from the host humoral and cell-mediated immune responses and antiviral treatment. Supported by the robust next generation sequencing techniques, recent studies have provided significant insights on the genomic diversity and progression as well as on the origin and the epidemiology of HCV. This review provides an overview of the mechanisms of HCV genetic variability, and the interactions with the host, that affects clinical disease, covering viral and host determinants of humoral and cell-mediated immune responses, alterations during the early and late stages of the infection and disease progression leading to chronicity. In addition, current findings in virus evolution and epidemiology were briefly interpreted from the inter-species and population perspectives. The impact of viral genomic heterogeneity on antiviral treatment in the era of direct-acting agents is also discussed, along with an overview of current methods employed for the characterization of viral diversity.Conference Object Real-World Evaluation of Anticoagulant Treatment Patterns in Patients With Atrial Fibrillation: Data From Multicenter Rota Study [2022](Kare Publ, 2023) Kocabas, Umut; Ergin, Isil; Yavuz, Veysel; Murat, Selda; Ozdemir, Ibrahim; Genc, Omer; Altin, Cihan; Özçalık, Emre; Şengör, Büşra Güvendi; Sen, Taner; Kaplan, Mehmet; Dal, Ahmet; Dogdus, Mustafa; Şahin, Şeyda[Abstract Not Available]Article Citation - WoS: 4Retrospective Evaluation of Candidemic Patients Among General Surgery Department in a Tertiary Care University Hospital(Turkish Surgical Assoc, 2019-09-23) Onal, Ugur; Metin, Dilek Yesim; Karaca, Can; Polat, Suleyha Hilmioglu; Ersin, Sinan; Tasbakan, Meltem IsikgozObjective: Candida species are among the most important causes of hospital acquired blood borne infections, and with high rates of mortality and morbidity, these infections are still a major problem today. History of gastrointestinal surgery, administration of total parenteral nutrition and/or wide spectrum antibiotics and immune suppression following organ transplantations are considered serious risk factors for these infections. This study aimed to evaluate the patients from our general surgery department with diagnosed candidemia; by means of strain, treatment and prognosis. Material and Methods: Patients with positive blood cultures for Candida species who were treated in the wards and Ege University Faculty of Medicine general surgery department of surgical intensive care units of our between 2012 and 2017 were retrospectively analyzed by means of strain, treatment and prognosis. Results: A total of 50 patients were enrolled in the study. Mean age was 58.96 years and 54% of the patients were female. There were nine patients with organ transplantation (four liver and five kidney transplantations), six with intestinal perforation and three with anastomotic leakage. Isolated strains were Candida albicans (36%; 18/50), Candida tropicalis (14%; 7/50), Candida glabrata (12%; 6/50), Candida parapsilosis (8%; 4/50), Candida kefyr (6%; 3/50), Candida krusei (4%; 2/50), Candida pulcherrima (2%; 1/50), Cryptococcus neoformans (2%, 1/50), Geotrichum capitatum (2%, 1/50), Candida spp. (unidentified, 14%; 7/50) with decreasing frequency. The highest antifungal sensitivity rates (> 90%) were measured for amphotericin B, voriconazole and echinocandins among all isolates. One-month mortality rate was 43.4% (20/46). Documented eradication was achieved among 24 of the 33 patients who had control blood culture samples (72.7%), and mean eradication time was 7.6 days. Echocardiography was performed in 14% (7/50) and ophthalmic examination in 8% (4/50). Conclusion: Although C. albicans appears to be the dominant strain in patients with candidemia, frequencies of other strains are increasing. Early diagnosis and treatment of patients with candidemia is of vital importance due to high mortality and morbidity rates.