TR Dizin İndeksli Yayınlar Koleksiyonu / TR Dizin Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/20.500.14365/4
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Article A Preliminary Study of Possible Fibrotic Role of Meprin Metalloproteases in Scleroderma Patients(Turkish League Against Rheumatism (TLAR), 2021-12-31) Avşar, Aydan Köken; Merih Birlik, A.; Koçak, Ayşe; Harmancı, Duygu; Akdoğan, Gül; Birlik, Merih; Birlik, A. Merih; Güner, Gül AkdoganObjectives: This study aims to investigate the possible fibrotic role of meprin metalloproteases and possible fibrotic effects of activator protein-1 (AP-1) in scleroderma patients. Patients and methods: Between April 2018 and April 2019, a total of 85 scleroderma patients (9 males, 76 females; mean age: 54.9 +/- 12.1 years; range, 22 to 80 years) who met the 2013 American College of Rheumatology/European League Against Rheumatism criteria and 80 healthy control individuals (10 males, 70 females; mean age 42.9 +/- 10.2 years; range, 19 to 65 years) were included. Patients' data and blood samples were collected. Messenger ribonucleic acid expressions of interleukin (IL)-6, AP-1 subunits, and tumor necrosis factor-alpha (TNF-alpha) were analyzed by quantitative real-time polymerase chain reaction. Serum meprin alpha and beta protein levels were analyzed using the enzyme-linked immunosorbent assay. Results: Meprin alpha and meprin beta protein levels increased in scleroderma patients. The AP-1 subunits (c-Fos, c-Jun), IL-6, and TNF-alpha increased in scleroderma patients, compared to controls. Conclusion: Our results provide evidence showing that increased meprins levels may be related to AP-1 levels and increased meprins levels may responsible for increased inflammatory TNF-alpha and IL-6 levels. All these data suggest meprins as promising therapeutic targets to restore the balance between inflammation and extracellular matrix deposition in scleroderma.Article İnfluenza A/B Virüs ve RSV Validasyon Standartlarının Dijital PCR ile Kantitasyonu(Ankara Microbiology Soc, 2025-07-26) Sayıner, Ayca Arzu; Bulgurcu, AlıhanMikrobiyolojik tanı laboratuvarlarında kullanılacak tanı testleri için kantitatif standartların kullanıldığı yöntem doğrulama (verification) veya geçerli kılma (validation) çalışmaları gereklidir. Nükleik asit testlerinde sentetik nükleik asit veya plazmit yerine tam virüs içeren standartların kullanılması; ekstraksiyon, revers transkripsiyon ve amplifikasyonu içerecek şekilde tanı testinin tüm basamaklarının gerçek yaşam koşullarında değerlendirilmesini sağlar. Solunum yolu virüsleri için nükleik asit testlerine yönelik ticari kantitatif standart materyaller sınırlıdır. Bu çalışmada; influenza A virüs (infA), influenza B virüs (infB) ve respiratuvar sinsityal virüs (RSV) için dijital polimeraz zincir reaksiyonu [digital polymerase chain reaction (dPCR)] kullanılarak, kantitatif nükleik asit standartları geliştirilmesi amaçlanmıştır. Çalışmada; RSV, infA, infB RNA pozitif olduğu bilinen nazofarengeal sürüntü örneklerinin havuzlanmasıyla hazırlanan örneklerdeki viral nükleik asit miktarı, ticari primer/prob setleri (Qiagen, Almanya) kullanılarak dPCR (QIAcuity, Qiagen) yöntemiyle belirlenmiştir. Nükleik asit ekstraksiyonu, ticari bir kit (Xi’an Tianlong Science&Technology Co, Çin) kullanılarak yapılmıştır. dPCR yönteminin infA, infB ve RSV için analitik duyarlılık (LoD) ve kantitasyon alt sınırı (LoQ), çalışma içi ve çalışmalar arası tekrarlanabilirliği ve doğrusallığı belirlenmiştir. dPCR ile çalışılan örnekler, kantitatif revers transkripsiyon gerçek zamanlı [quantitative reverse transcription realtime (qrRT)] PCR (qRT-PCR) ile de çalışılarak Ct değerleri belirlenmiştir. Ct değerleri ile dPCR-kantitasyon sonuçları arasındaki ilişki lineer regresyon ile değerlendirilmiştir. İstatistiksel analiz GraphPad Prism 10.4.0 (GraphPad, ABD) ve Excel Analysis ToolPak kullanılarak yapılmıştır. İnfA, infB ve RSV için dPCR yönteminin LoD değerleri sırasıyla 93.75, 15.59 ve 26.23 kopya/mL olarak belirlenmiştir. dPCR yönteminin çalışma içi tekrarlanabilirliği (varyasyon katsayısı, %CV), düşük viral yükü olan örneklerde daha yüksek olmak üzere 0.06-7.97 arası saptanmıştır. Çalışmalar arası tekrarlanabilirlik 0.73-5.41 olarak bulunmuştur. İnfA ve infB için 3-4 log10, RSV için 7 log10 aralığında dilüsyonlar ile yapılan doğrusallık analizinde her üç virüs için de r 2≥ 0.99 olarak bulunmuştur. dPCR ile ölçülen konsantrasyonların, qRT-PCR Ct sonuçları ile korele olduğu saptanmıştır. dPCR ile qRT-PCR testlerinin çalışma içi ve çalışmalar arası tekrarlanabilirlik sonuçları karşılaştırıldığında, dPCR’nin %CV değerinin anlamlı olarak daha düşük olduğu saptanmıştır (p= 0.0312). Çalışma sonuçları dPCR yönteminin, kantitatif nükleik asit standartları elde etmede tekrarlanabilirliği yüksek ve güvenilir bir yöntem olduğunu göstermiştir. Elde edilen kantitatif standartlar ile viral yük belirlemeye yönelik tanı yöntemleri geliştirmek ve/veya bu tür testlerin yöntem onayı analizlerini yapmak mümkündür. Sonuç olarak çalışmada, havuzlanmış hasta örnekleri kullanılarak dPCR yöntemiyle infA, infB ve RSV için güvenilir kantitatif nükleik asit standartlar elde edilmiş ve dPCR yönteminin performans analizleri gerçekleştirilmiştir. Bu çalışma, dPCR ile kantitatif viral nükleik asit standartlarının üretimine bir örnek olmuştur.Article Expressions of the Satellite Repeat Hsat5 and Transposable Elements Are Implicated in Disease Progression and Survival in Glioma(Tubitak Scientific & Technological Research Council Turkey, 2024-08-23) Köse, Sıla Naz; Yaraş, Tutku; Bursalı, Ahmet; Oktay, Yavuz; Yandım, Cihangir; Karakulah, GökhanThe glioma genome encompasses a complex array of dysregulatory events, presenting a formidable challenge in managing this devastating disease. Despite the widespread distribution of repeat and transposable elements across the human genome, their involvement in glioma's molecular pathology and patient survival remains largely unexplored. In this study, we aimed to characterize the links between the expressions of repeat/transposable elements with disease progression and survival in glioma patients. Hence, we analyzed the expression levels of satellite repeats and transposons along with genes in low-grade glioma (LGG) and high-grade glioma (HGG). Endogenous transposable elements LTR5 and HERV_a-int exhibited higher expression in HGG patients, along with immune response-related genes. Altogether, 16 transposable elements were associated with slower progression of disease in LGG patients. Conversely, 22 transposons and the HSAT5 satellite repeat were linked to a shorter event-free survival in HGG patients. Intriguingly, our weighted gene coexpression network analysis (WGCNA) disclosed that the HSAT5 satellite repeat resided in the same module network with genes implicated in chromosome segregation and nuclear division; potentially hinting at its contribution to disease pathogenesis. Collectively, we report for the first time that repeat and/or transposon expression could be related to disease progression and survival in glioma. The expressions of these elements seem to exert a protective effect during LGG-to-HGG progression, whereas they could have a detrimental impact once HGG is established. The results presented herein could serve as a foundation for further experimental work aimed at elucidating the molecular regulation of glioma genome.Letter Citation - WoS: 1Citation - Scopus: 1About The Article Titled “a Different Scintigraphic Perspective On The Systolic Function Of The Left Ventricle-1” [“sol Ventrikül Sistolik Fonksiyonuna Sintigrafik Olarak Farklı Bir Bakış Açısı-1” Başlıklı Makale Hakkında](Galenos Publishing House, 2024-02-23) Taşçı, Cengiz; Tascs, Cengiz[No abstract available]Article Citation - WoS: 7Citation - Scopus: 7Effectiveness of a Program To Raise Awareness About Pneumococcal Vaccination Among Physicians and Patients With Chronic Respiratory Diseases: a Multicenter Cohort Study(AVES, 2024-08-27) Karakurt, Z.; Yalnız, E.; Altın, S.; Oruç, Ö.; Uslu, Ö.; Veske, N.Ş.; Kılınç, O.; Sayıner, Abdullah; Kul, Seval; Şimşek, NurdanOBJECTIVE: There is a need to increase patient and clinician awareness on the effectiveness of pneumococcal vaccination in at-risk groups. The aim of the study was to evaluate the effect of reminders for physicians and patients using the vaccination tracking system created in the hospital information management system (HIMS) on the vaccination rate, and the effect of pneumococcal vaccination on pneumonia-related hospitalization and mortality over a 12-month period. MATERIAL AND METHODS: This prospective observational cohort study was undertaken during a 2-year period in 3 tertiary care cen-ters. Patients were followed up for 12 months following vaccination, and hospital admissions and mortality were recorded via HIMS. During the campaign, HIMS transmitted pneumococcal immunization reminder messages in accordance with guideline recommendations to physicians’ computers and patients’ mobile phones. Educational posters on pneumococcal vaccination were posted in outpatient clinics. Regular seminars on the evidence for pneumococcal vaccination were organized. All patients who were hospitalized during the follow-up period for chronic obstructive pulmonary disease (COPD), asthma, lung cancer, or pneumonia were analyzed in relation to their vaccination history regarding clinical outcomes. RESULTS: A total of 29 530 patients were included in the study. During the study period, the annual vaccination rate increased by 74.4% and reached 4.8% in 3 hospitals (P = .001). The rates were 3.9% in patients older than 65 years without comorbidities and 5.2% in those with COPD and asthma (P = .002). In pneumococcal vaccine recipients, pneumonia-related hospital mortality was lower (relative risk (RR) = 0.19, CI 0.09-0.35, P < .001). CONCLUSION: It is possible to raise the rate of pneumococcal vaccination through awareness campaigns. Individuals with COPD and asthma are more willing to receive pneumococcal vaccination. Among patients hospitalized for pneumonia, prior pneumococcal vaccination is associated with lower mortality. © Author(s).Article Citation - WoS: 2Citation - Scopus: 2Adrenomedullin Has No Effect on Segmental Bone Defect Healing but Increases Bone Mineral Density in Rat Model(AVES, 2023-10-11) Kaymakoğlu, Mehmet; Ciftci, E.; Korkusuz, P.; Ozdemir, E.; Erden, M.E.; Turhan, E.Objective: This study aimed to investigate the effect of adrenomedullin on the healing of the segmental bone defect in a rat model. Methods: Thirty-six Wistar rats were randomly divided into 6 groups based on follow-up periods and administered a dose of adreno-medullin hormone. In each group, bilaterally, a 2-mm bone defect was created at the diaphysis of the radius. Sodium chloride solution was administered to sham groups 3 times a week for 4 and 8 weeks intraperitoneally. Adrenomedullin was administered to the study groups 3 times a week: 15 μg—4 weeks, 15 μg—8 weeks, 30 μg—4 weeks, and 30 μg—8 weeks, respectively. After euthanasia, the segmental defects were evaluated by histomorphometric [new bone area (NBA)] and microtomographic [bone volume (BV), bone surface (BS), and bone mineral density (BMD)] analyses. Results: Although the 4-and 8-week 15 μg administered study groups had higher NBA values than the other study and control groups, the histomorphometric analysis did not reveal any statistical difference between the control and study groups regarding NBA (P >.05). In microtomographic analysis, BV was higher in the 15 μg 4-week group than 30 μg 4-week group (296.9 vs. 208.5, P =.003), and BS was lower in the 30 μg 4-week group than in the 4-week control group (695.5 vs. 1334.7, P =.005), but overall, no significant difference was found between the control and study groups (P >.05). Despite these minor differences in histomorphometric and microtomographic criteria indicating new bone formation, the BMD values of the 15 μg 8-week study group showed a significant increase compared with the control group (P =.001, respectively). Conclusion: Adrenomedullin positively affected BMD at 15 μg, but this study could not show healing in the segmental defect site at different dose regimens. Further studies are needed to assess its effects on bone tissue trauma. © 2023, AVES. All rights reserved.Article Citation - Scopus: 1Course of Treatment of Chronic Bleeding and Anemia with Systemic Bevacizumab Treatment in Hereditary Hemorrhagic Telangiectasia: A Retrospective Cohort(Türkiye Klinikleri, 2023) Durak, Emine; Ugur, Mehmet Can; Gediz, FusunAmaç: Kalıtsal hemorajik telenjiyektazi (HHT), anormal anjiyo- geneze neden olan otozomal dominant bir hastal ıktır. Son y ıllarda, özel- likle şiddetli HHT formlarının tedavisi için hedefe yönelik tedavilere olan ilgi artmaktadır. Bu tedavi seçeneklerinden biri de vasküler endotelyal bü- yüme faktörü inhibitörü bevasizumabtır. Bu çalışmanın amacı, HHT tanısı alan hastalarda sistemik bevasizumab kullanımının kronik kanama seyri ve anemi tedavisindeki etkisini ara ştırmaktır. Gereç ve Yöntemler: Bevasi- zumab kullanan hastaların tedavi yanıtı ve yan etkileri retrospektif olarak değerlendirildi. Bulgular: Hastaların yaş ortalaması 51,5 yıl idi. Ortalama tedavi süresi 15,4 (4-25 ay) aydı. Tüm hastalara 2 hafta arayla ilk 4 doz 5 mg/kg intravenöz bevasizumab uygulandı. Bevasizumab idamesi 5 mg/kg dozunda aylık periyotlarla devam etti. Bevasizumab tedavisi ile hemoglo- bin değerlerinde artış, epistaksis şiddet skorunda parenteral demir ve erit- rosit transfüzyon gereksiniminde azalma sağlandı. Gözlenen yan etkiler, 1 hastada alerjik döküntü ve 1 hastada artralji idi. Yan etkiler nedeniyle hiç- bir hastada tedavinin kesilmesi gerekmedi. Sonuç: Bevasizumab, kontrol edilmediği takdirde ölümcül olabilen HHT’de umut verici bir tedavi seçe- neğidir. Bununla birlikte, tedavi protokolleri ve advers olayların yönetimi konusunda global konsensus sağlamak için daha kapsamlı çalışmalara ih- tiyaç vardır.Article Citation - WoS: 5Citation - Scopus: 5The Effects of Playing Digital Games on Children's Pain, Fear, and Anxiety Levels During Suturing: a Randomized Controlled Study(Wolters Kluwer Medknow Publications, 2023-07) Kavlakcı, Muhammet; Öğce, Filiz; Yavan, TulayOBJECTIVE: The aim was to determine the effects of digital game play on children's pain, fear, and anxiety levels during suturing. METHODS: Data were obtained from 84 children between the ages of 8 and 17 years at the pediatric emergency department between January 16 and March 19, 2020, using the Socio-Demographic and Clinical Characteristics Form, the Wong-Baker Faces Pain Rating Scale (WBFPS), the Visual Analogue Scale (VAS), the Fear of Medical Procedures Scale (FMPS), and the State-Trait Anxiety Inventory for Children (STAI-CH). A four-block randomization system was used. The study group (n = 42) played digital games during the suturing procedure, unlike the control group (n = 42). Ethical permissions were obtained from the ethical committee, hospital, and families. RESULTS: Before the suturing procedure, there was no statistically significant difference between the groups' mean scores. The intervention group was found to have statistically significantly lower WBFPS and VAS pain scores than the control group during the suturing procedure, and after the procedure, statistically significantly lower WBFPS, VAS, FMPS, and STAI-CH mean scores than the control group. CONCLUSIONS: The digital game-playing approach applied before and during the suture procedure was found to be effective in reducing children's pain, fear, and anxiety levels.Article Citation - WoS: 1Citation - Scopus: 1Shotgun Lipidomics Elucidates the Lipidome Alterations of the Mcl-1 Inhibitor S63845 in Aml Cell Lines With a Focus on Sphingolipids(Istanbul University Press, 2022-12-30) Yandım, Melis Kartal; Bilgin, MesutObjective: Acute myeloid leukemia (AML) is a vigorous type of leukemia requiring effective treatment. Myeloid cell leukemia-1 (Mcl-1) is an anti-apoptotic molecule that is upregulated in AML and is studied as a target for treatment. The specific Mcl-1 inhibitor, S63845, has antiproliferative effects on AML cells. Bioactive sphingolipids have crucial roles in cells and regulate Mcl-1 stability. This study aimed to elucidate the changes in lipid profiles of AML cell lines in response to Mcl-1 inhibitor S63845 treatment, with a special focus on sphingolipids. Materials and Methods: The cytotoxic effects of S63845 were identified in the AML cell lines MV4-11, HL60, and KG1 using the MTT cell proliferation assay. Lipidome analysis was conducted by quantitative shotgun lipidomics covering 378 individual lipid species in 26 classes within the major lipid categories. Results: The IC50 values of S63845 have been calculated as 7 nM for MV4-11, 53 nM for HL60, and 479 nM for KG1. The lipidome results reveal the S63845 treatment to increase ceramide (Cer) levels in the MV4-11 and KG1 cell lines at the expense of downstream sphingolipids while increasing the hexosylceramide (HexCer) levels in the HL60 cell line at the expense of the Cer and sphingomyelin (SM). Conclusion: This study showed S63845 to be able to suppress cell proliferation by altering lipid compositions in AML cell lines. More importantly, the study suggested S63845 to differentially affect the lipid profiles of AML cell lines.Article Investigation of Neuraminidase 1 Gene Association in Henoch-Schönlein Purpura (hsp) With Renal Involvement(Pamukkale University, 2022-07-01) Yılmaz, N.B.; Ertan, P.; Yüksel, S.; Neşe, N.; Dinç Horasan, Gönül; Berdeli, A.H.; Horasan, Gönül Dinç; Bahçeci, Nezihe BilgePurpose: HSP is a common small vessel vasculitis. It is the most common cause of non-thrombocytopenic purpura in childhood. The role of genes in etiopathogenesis of the disease, which has not yet been clearly elucidated, is being emphasized. Many genes called sialidases are being studied and is thought that the NEU1 gene may be particularly important in the etiopathogenesis of HSP. The aim of this study is to investigate the role of the NEU1 gene in the etiopathogenesis of HSP and its relation to renal involvement. Materials and methods: Fifty patients followed in the Celal Bayar University Hafsa Sultan Hospital Pediatric Nephrology Department, with the diagnosis of HSP renal involvement were included into the study. For the control group, age and gender matched 50 cases were accepted among the outpatients admitted to Pediatric Department without any chronic diseases. NEU1 gene mutation analysis was performed in blood samples of both patient and control groups by using the Sanger DNA sequencing method. Results: NEU1 genetic mutation was not detected in any HSP patient with renal involvement and control group. Conclusion: In our study, the NEU 1 gene was not found to be associated with HSP nephritis. No changes were detected in the investigated regions of the NEU1 gene. © 2022, Pamukkale University. All rights reserved.