01. Araştırma Çıktıları | TR-Dizin | WoS | Scopus | PubMed

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24 Saatlik İdrarda Protein Atılımının Tanılayıcı Kullanımı ve Advers Perinatal Sonuçlar ile Doğum Zamanı için Protein-kreatinin Oranı
(2021) Gölbaşı, Hakan; Ömeroğlu, İbrahim; Dereli Akdeniz, Didem; Ekin, Atalay; Gölbaşı, Ceren
Amaç: Çal›flmam›zda, proteinürisi olan veya olmayan hipertansif gebelerde perinatal ve neonatal sonuçlar› de¤erlendirmeyi amaçla- d›k. Spot üriner protein ile kreatinin oran›n›n (P/C) ve 24-saatlik protein at›l›m›n›n sonuçlar üzerindeki prediktivitesini karfl›laflt›r- d›k. Yöntem: Gebeli¤in 20. ve 37. haftalar› aras›nda yeni hipertansiyon tan›s› alm›fl 230 gebe retrospektif olarak çal›flmaya dahil edildi. Al›- nan 24 saatlik idrar ve P/C ile belirlenen protein seviyesine göre hastalar iki gruba ayr›ld›. Proteinüri varl›¤› ve seviyesi, P/C oran›y- la olan iliflkisi ve bu bulgular ile perinatal sonuçlar aras›ndaki iliflki de¤erlendirildi. Bulgular: Do¤um esnas›ndaki gestasyonel yafl ve gecikme dönemi (hipertansiyon tan›s› ile do¤um aras›ndaki süre), ?300 mg/24 saat ve P/C ?0.3 de¤erlerine sahip gebelerde anlaml› flekilde daha er- kendi. Advers neonatal sonuçlar, proteinürisi ?300 mg/24 saat ve P/C?0.3 olan hastalarda anlaml›yd›. 24 saatlik idrarda üriner pro- tein seviyeleri, P/C ?0.3 de¤erine sahip gebelerde anlaml› flekilde daha yüksekti ve 24 saatlik proteinüri ile P/C aras›nda anlaml› fle- kilde pozitif bir korelasyon bulundu (r=0.382, p<0.001). Sonuç: Çal›flmam›z, hipertansif gebelerde 24 saatte ?300 mg’lik bir protein kayb› ve spot idrarda ?0.3’lük bir P/C oran›n›n advers perinatal sonuçlarla iliflkili oldu¤unu ortaya koymufltur. Ayr›ca, hi- pertansif gebelerde ?300 mg/gün seviyesinde proteinürinin ve ?0.3’lük spot idrar P/C oran›n›n bu gebeleri erken do¤um riskine e¤ilimli k›ld›¤›n› tespit ettik.
Citation - WoS: 1
Citation - Scopus: 1
25-Hydroxyvitamin Levels in Sjögren’s Syndrome: Is It the Right Time to Dismiss the Case or Not
(Walter de Gruyter GmbH, 2024) Sımsır, Ilgın Yıldırım; Tanigor, Goksel; Karabulut, Gonca; Barutcuoglu, Burcu; Yılmaz, Zevcet
Objectives: This study aimed to investigate whether patients with primary Sjögren syndrome (SjS) have different levels of 25 OH-D3 (vitamin D) when compared to healthy populations and whether differences in 25 OH-D3 correlated with disease activity or markers. Methods: Eighty-eight female patients with SjS and 3,338 age-matched healthy female controls were included in this study. 25 OH-D3 levels were compared with healthy controls. Then the patients were stratified according to their 25 OH-D3 levels, either insufficient/deficient or normal (<50 nmol/L or ≥50 nmol/L). The disease activity was evaluated using The EULAR SjS disease activity index (ESSDAI) and its components. Correlation analyses were also performed for a possible correlation with disease characteristics and markers of activity. Results: No differences in 25 OH-D3 levels were found between SjS and healthy populations (p>0.05). No correla- tions were found between patient characteristics or labo- ratory values (p>0.05). Conclusions: This study did not find a link between disease characteristics and disease activity and 25 OH-D3 levels. Prospective studies with more patients should be conducted to reach a conclusion.
Citation - Scopus: 3
3d Printing With Bacterial Cellulose-Based Bioactive Composites for Design Applications
(Education and research in Computer Aided Architectural Design in Europe, 2022) Turhan G.D.; Afsar S.; Ozel B.; Doyuran A.; Varinlioglu G.; Bengisu, Murat
The bacterial cellulose (BC) biofilms are explored in design applications as replacements to petroleum-based materials in order to overcome the irreversible effects of the Anthropocene. Unlike biomaterials, designers as mediators could collaborate with bioactive polymers as a form of wetware to manufacture living design products with the aid of novel developments in biology and engineering. Past and ongoing experiments in the literature show that BC has a strong nanofibril structure that provides adhesion for attachment to plant cellulose-based networks and it could grow on the surfaces of the desired geometry thanks to its inherited, yet, controllable bio-intelligence. This research explores BC-based bioactive composites as wetware within the context of digital fabrication in which the methodology involves distinct, yet integrated, three main stages: Digital design and G-code generation (software stage); BC cultivation and printable bioactive composite formulation (wetware stage); digital fabrication with a customized 3D printer (hardware stage). The results have shown that the interaction of BC and plant-based cellulose fibers ofjute yarns has enhanced the structural load-bearing capacity of the form against compressive forces, while pure BC is known only by its tensile strength. Since the outcomes were fabricated with the use of a bioactive material, the degradation process also adds a fourth dimension: Time, by which the research findings could further establish a bio-upcycling process of wastes towards biosynthesis of valuable products. Moreover, developing a BC-based bioactive filament indicates potentially a feasible next step in the evolution of multiscale perspectives on the growth of habitable living structures that could reinforce the interaction between nature and architecture through collaboration with software, hardware, and wetware in innovative and sustainable ways. © 2022, Education and research in Computer Aided Architectural Design in Europe. All rights reserved.
3d Printing With Bacterial Cellulose-Based Bioactive Composites for Design Applications
(Ecaade-education & research computer aided architectural design europe, 2022) Turhan, Gözde Damla; Afşar, Seçil; Özel, Berfin; Doyuran, Aslıhan; Varinlioglu, Güzden; Bengisu, Murat
The bacterial cellulose (BC) biofilms are explored in design applications as replacements to petroleum-based materials in order to overcome the irreversible effects of the Anthropocene. Unlike biomaterials, designers as mediators could collaborate with bioactive polymers as a form of wetware to manufacture living design products with the aid of novel developments in biology and engineering. Past and ongoing experiments in the literature show that BC has a strong nanofibril structure that provides adhesion for attachment to plant cellulose-based networks and it could grow on the surfaces of the desired geometry thanks to its inherited, yet, controllable bio-intelligence. This research explores BC-based bioactive composites as wetware within the context of digital fabrication in which the methodology involves distinct, yet integrated, three main stages: Digital design and G-code generation (software stage); BC cultivation and printable bioactive composite formulation (wetware stage); digital fabrication with a customized 3D printer (hardware stage). The results have shown that the interaction of BC and plant-based cellulose fibers of jute yarns has enhanced the structural load-bearing capacity of the form against compressive forces, while pure BC is known only by its tensile strength. Since the outcomes were fabricated with the use of a bioactive material, the degradation process also adds a fourth dimension: Time, by which the research findings could further establish a bio-upcycling process of wastes towards biosynthesis of valuable products. Moreover, developing a BC-based bioactive filament indicates potentially a feasible next step in the evolution of multiscale perspectives on the growth of habitable living structures that could reinforce the interaction between nature and architecture through collaboration with software, hardware, and wetware in innovative and sustainable ways.
Citation - WoS: 2
Accidental Humor in Consecutive Interpreting: Accidentally Useful Food for Thought
(Istanbul Univ, Fac Letters, 2021) Yetkin Karakoç, Nihal
Unlike humor translation, humor in translation, caused by translation errors, is underresearched in translation studies. Accidental humor, as a subgroup of humor, has been analyzed in translation contexts, but to the best knowledge of the author, not in the interpreting context. This descriptive study, being the first of its kind dealing with the accidental humor in consecutive interpreting, is intended to fill the gap in the verbal manifestations of resources in the English-Turkish interpreting context along with the possible reasons for resources of accidental humor in consecutive interpreting class. As a result of the study, a total of 38 accidentally humorous instances are found. The verbal resources are analyzed and divided into main and sub-categories, and for each, examples from the corpus are discussed. In addition, new linguistic resources were discovered, which may be exclusive to this context. The detected items are by no means meant to be exhaustive, as also stated by the former studies, but nevertheless enables greater understanding about the types and possible causes of accidental humor which may occur during the consecutive interpreting process. Given the dearth of literature on accidental humor in consecutive interpreting, this study may provide some insight for remedial teaching/learning in interpreting studies, as well as linguistics. Future studies may examine, among the others, verbal resources in the simultaneous interpreting context and compare the verbal resources in depth in consecutive with those in simultaneous interpreting.
Citation - WoS: 6
Citation - Scopus: 5
Acrylamide-Encapsulated Glucose Oxidase Inhibits Breast Cancer Cell Viability
(Walter De Gruyter Gmbh, 2020) Rrustemi, Trendelina; Geyik, Oyku Gonul; Ozkaya, Ali Burak; Ozturk, Taylan Kurtulus; Yuce, Zeynep; Kilinc, Ali
Objectives: Cancer cells modulate metabolic pathways to ensure continuity of energy, macromolecules and redoxhomeostasis. Although these vulnerabilities are often targeted individually, targeting all with an enzyme may prove a novel approach. However, therapeutic enzymes are prone to proteolytic degradation and neutralizing antibodies leading to a reduced half-life and effectiveness. We hypothesized that glucose oxidase (GOX) enzyme that catalyzes oxidation of glucose and production of hydrogen peroxide, may hit all these targets by depleting glucose; crippling anabolic pathways and producing reactive oxygen species (ROS); unbalancing redox homeostasis. Methods: We encapsulated GOX in an acrylamide layer and then performed activity assays in denaturizing settings to determine protection provided by encapsulation. Afterwards, we tested the effects of encapsulated (enGOX) and free (fGOX) enzyme on MCF-7 breast cancer cells. Results: GOX preserved 70% of its activity following encapsulation. When fGOX and enGOX treated with guanidinium chloride, fGOX lost approximately 72% of its activity, while enGOX only lost 30%. Both forms demonstrated remarkable resilience against degradation by proteinase K and inhibited viability of MCF-7 cells in an activity-dependent manner. Conclusions: Encapsulation provided protection to GOX against denaturation without reducing its activity, which would prolong half-life of the enzyme when administered intravenously.
Citation - WoS: 1
Activating Co-Creation Methodologies of 3d Printing With Biocomposites Developed From Local Organic Wastes
(Ecaade-education & research computer aided architectural design europe, 2022) Afsar, Seçil; Estevez, Alberto T.; Abdallah, Yomna K.; Turhan, Gözde Damla; Özel, Berfin; Doyuran, Aslıhan
Compared to the take-make-waste-oriented linear economy model, the circular model has been studied since the 1980s. Due to consumption-oriented lifestyles along with having a tendency of considering waste materials as trash, studies on sustainable materials management (SMM) have remained at a theoretical level or created temporary and limited impacts. To ensure SMM supports The European Green Deal, there is a necessity of developing top-down and bottom-up strategies simultaneously, which can be metaphorized as digging a tunnel from two different directions to meet in the middle of a mountain. In parallel with the New European Bauhaus concept, this research aims to create a case study for boosting bottom-up and data-driven methodologies to produce short-loop products made of bio-based biocomposite materials from local food & organic wastes. The Architecture departments of two universities from different countries collaborated to practice these design democratization methodologies using data transfer paths. The 3D printable models, firmware code, and detailed explanation of working with a customized 3D printer paste extruder were shared using online tools. Accordingly, the bio-based biocomposite recipe from eggshell, xanthan gum, and citric acid, which can be provided from local shops, food & organic wastes, was investigated concurrently to enhance its printability feature for generating interior design elements such as a vase or vertical gardening unit. While sharing each step from open-source platforms with adding snapshots and videos allows further development between two universities, it also makes room for other researchers/makers/designers to replicate the process/product. By combining modern manufacturing and traditional crafting methods with materials produced with DIY techniques from local resources, and using global data transfer platforms to transfer data instead of products themselves, this research seeks to unlock the value of co-creative design practices for SMM.
Citation - Scopus: 2
Activating Co-Creation Methodologies of 3d Printing With Biocomposites Developed From Local Organic Wastes
(Education and research in Computer Aided Architectural Design in Europe, 2022) Afsar S.; Estevez A.T.; Abdallah Y.K.; Turhan G.D.; Oze B.; Doyuran A.
Compared to the take-make-waste-oriented linear economy model, the circular model has been studied since the 1980s. Due to consumption-oriented lifestyles along with having a tendency of considering waste materials as trash, studies on sustainable materials management (SMM) have remained at a theoretical level or created temporary and limited impacts. To ensure SMM supports The European Green Deal, there is a necessity of developing top-down and bottom-up strategies simultaneously, which can be metaphorized as digging a tunnel from two different directions to meet in the middle of a mountain. In parallel with the New European Bauhaus concept, this research aims to create a case study for boosting bottom-up and data-driven methodologies to produce short-loop products made of bio-based biocomposite materials from local food & organic wastes. The Architecture departments of two universities from different countries collaborated to practice these design democratization methodologies using data transfer paths. The 3D printable models, firmware code, and detailed explanation of working with a customized 3D printer paste extruder were shared using online tools. Accordingly, the bio-based biocomposite recipe from eggshell, xanthan gum, and citric acid, which can be provided from local shops, food & organic wastes, was investigated concurrently to enhance its printability feature for generating interior design elements such as a vase or vertical gardening unit. While sharing each step from open-source platforms with adding snapshots and videos allows further development between two universities, it also makes room for other researchers/makers/designers to replicate the process/product. By combining modern manufacturing and traditional crafting methods with materials produced with DIY techniques from local resources, and using global data transfer platforms to transfer data instead of products themselves, this research seeks to unlock the value of co-creative design practices for SMM. © 2022, Education and research in Computer Aided Architectural Design in Europe. All rights reserved.
Citation - WoS: 2
Citation - Scopus: 1
Adjuvant Effects of Chemotherapeutics and Metformin on Mfe-319 Endometrial Carcinoma Cell Line
(Editura Acad Romane, 2020) Aydemir, Isil; Uluer, Elgin Turkoz; Korkmaz, Oya; Tuglu, Mehmet Ibrahim; Inan, Sevinc
We aimed to investigate the cytotoxicity of Metformin, Cisplatin, and Paclitaxel on MFE-319 endometrial carcinoma cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and immunocytochemistry assays. Half maximal inhibitory concentration (IC50) doses of three drugs alone and in the dual combinations were applied to the cells. Immunocytochemical method was performed for the cell survival and for phosphatidylinositol 3-kinase (PI3K), phosphorylated extracellular regulated kinases (pErk)-1/2, Akt-1, phosphorylated Akt (pAkt)-1/2/3 cell growth markers and angiogenic vascular endothelial growth factor (VEGF). Immunoreactivities were evaluated using H-score and analyzed using the one-way analysis of variance (ANOVA) test for statistics. It was found that these drugs caused a decrease in the immunoreactivities of these markers. Particularly, dual combination of Paclitaxel and Cisplatin decreased the immunoreactivities of PI3K, pErk-1/2, Akt-1, and pAkt-1/2/3. Cisplatin and Paclitaxel were more effective than Metformin; on the other hand, Metformin has been shown to enhance the efficacy of these two drugs. In vitro or in vivo further studies are needed to investigate the efficacy of these three drugs via PI3K/Akt signal pathway.
Aerospace 3d Textile Preform Analysis: Experimental and Mesomodeling
(Polish Society of Composite Materials, 2023) Saboktakin, Abbasali; Safaei, H.
For the purpose of predicting how textile preforms affect the quality of the composite material and its performance, the stitched textile preform must be characterized. Experimental compaction analysis and finite element analysis of textile preforms are the main subjects of this paper. The formability parameters of a preform can be changed by the stitching process, according to research on the mechanical properties of preforms conducted during compression testing. The load-deformation response, which is depicted in detail, had the greatest influence on preform deformation. Less fiber bundle undulation in the plane direction and more stitching thread undulation in the thickness direction were observed during compression of the stitched preform, whereas the stitching thread improved the resistance of the preform to compression loading. © 2023, Polish Society of Composite Materials. All rights reserved.
Citation - Scopus: 1
Affecting Factors in Medication Adherence in Kidney Transplant Recipients
(Aves, 2023) Ordin, Yaprak Sarigol; Karayurt, Ozgul; Ertan, Nermin; Edeer, Aylin Durmaz; Yildiz, Serkan
Objective: This study aimed to determine the risk factors related to immunosuppressive medication adherence in kidney transplant recipients. Methods: The study adopted a retrospective design. It was carried out on 208 kidney transplant recipients. The social cognitive theory was used for understanding and addressing the issues of nonadherence to immunosuppressive medication. Data were collected with a self-reported-Immunosuppressant Therapy Adherence Scale and biological assays. Descriptive analyses and logistic regression were used to analyze data. Results: According to the self-reported assessment, medication adherence of the patients receiving transplants from live donors was 0.503 times lower than that of the patients receiving transplants from deceased donors. The biological assays did not show any affecting factors in medication adherence. Conclusion: The results of the study showed the importance of combining methods to assess medication adherence. In the self-reported assessment, live donor transplantation was found to negatively affect medication adherence. Turkiye is the country with the highest number of kidney transplantations from live donors in the world. Health professionals must be more careful in assessing medication adherence after live donor transplantations.
Citation - Scopus: 1
Affective Computing for Game User Research
(Education and research in Computer Aided Architectural Design in Europe, 2023) Varinlioğlu, Güzden; Tu, H.; Nagakura, T.
This study examines the spatial and emotional experience facilitated by immersive gaming environments and detected by wearable technologies. We designed, implemented, and tested a serious board game involving simulating the urban networks of the trade routes of the past. We recorded the traces of the players’ collective movements using a ceiling-mounted video camera to record the positions of the markers, and a video camera facing the players to record the players’ behaviors. Wearable devices enabled the recording of players’ emotions during their decision-making process. We captured their emotions with three tools: a GSR to capture participants’ emotional state and arousal level, an EEG to record the changes in their brain activity; and a think-aloud protocol to understand their conscious decisions. Finally, we analyzed the gamers’ measured "feelings" and compared these with the expressed feelings. The longer-term goal is to explore architectural design with special attention to feelings experienced within it by means of affective computing methods such as wearable technologies and biosensors, as well as gamification. © 2023, Education and research in Computer Aided Architectural Design in Europe. All rights reserved.
Affective Computing for Game User Research
(Ecaade-education & research computer aided architectural design europe, 2023) Varınlıoğlu, Güzden; Tu, Han; Nagakura, Takehiko
This study examines the spatial and emotional experience facilitated by immersive gaming environments and detected by wearable technologies. We designed, implemented, and tested a serious board game involving simulating the urban networks of the trade routes of the past. We recorded the traces of the players' collective movements using a ceiling-mounted video camera to record the positions of the markers, and a video camera facing the players to record the players' behaviors. Wearable devices enabled the recording of players' emotions during their decision-making process. We captured their emotions with three tools: a GSR to capture participants' emotional state and arousal level, an EEG to record the changes in their brain activity; and a think-aloud protocol to understand their conscious decisions. Finally, we analyzed the gamers' measured feelings and compared these with the expressed feelings. The longer-term goal is to explore architectural design with special attention to feelings experienced within it by means of affective computing methods such as wearable technologies and biosensors, as well as gamification.
Agile Approach as an Answer To Internal Uncertainty: a Case Study in Large-Scale Manufacturing
(Inderscience Publishers, 2023) Özgen, A.; Gençer, Mehmet
The success of agile product development in the software industry has attracted the attention of new product development (NPD) community. Agile approach has the potential to overcome the uncertainty that dominates today’s business conditions. However, there is little research regarding the performance of agile practices, most of which were developed in the software industry, in other contexts. Through a case study methodology, we explore how agile practices address organisational uncertainty in a large-scale manufacturing firm. The study, based on semi-structured interviews, covers two NPD projects with a total of 12 sub-teams. Our results indicate that in a large organisation, internal sources of NPD uncertainty dominate external sources. The three factors in organisational uncertainties are interdependency, team volatility and distributed teams. Furthermore, we develop a model to explain how various agile practices can provide the NPD teams with capabilities to mitigate these organisational uncertainties. Copyright © 2023 Inderscience Enterprises Ltd.
Aile Hekimlerinin Sağlıklı Yaşam Biçimi Davranışlarının ve Klinik Uygulamalarında Yaşam Biçimi Tıbbının Değerlendirilmesi
(Galenos Publishing House, 2025) Semin, Ilgi; Kazdağlı, Hasan; Gökdemir, Özden
Amaç: Bu çalışmanın amacı, aile hekimlerinin sağlıklı yaşam biçimi davranışlarını değerlendirmek ve klinik uygulamalarında yaşam biçimi tıbbına yönelik katılımlarını incelemektir. Yöntemler: Temmuz 2018 ile Ağustos 2019 tarihleri arasında aile hekimlerinden veri toplamak amacıyla çevrimiçi bir anket geliştirildi. Tanımlayıcı ve korelasyonel bir araştırma deseni kullanıldı. Çalışmada uygulanan anket, “katılımcıların kişisel bilgileri” ve “Sağlıklı Yaşam Biçimleri Davranış Ölçeği-II”i içermektedir. Kesitsel bir çalışma olarak tasarlanan bu araştırmada, örneklem kolayda örnekleme metodu ile sekiz ülkeden belirlenmiştir, ve anketler katılımcılara elektronik ortamda uygulanmıştır. Katılımcılara anketin süresi, veri saklama işlemleri, saklama süresi, araştırmacıların kimliği ve çalışmanın amacı hakkında bilgi verilmiştir. Bulgular: Çalışmaya 131 aile hekimi katılmış olup, erkek katılımcı oranı daha yüksek bulunmuştur. Sigara içme sıklığının Türk aile hekimleri arasında anlamlı derecede yüksek olduğu tespit edilmiştir (p=0,013). Ancak, ölçek toplam puanı veya altı boyutta alınan puanlar açısından aile hekimleri arasında istatistiksel olarak anlamlı bir fark bulunmamıştır. Sonuç: Çoğu aile hekimi, bu alanda daha fazla eğitim almak istediklerini belirtmiştir, bu da tıp fakültelerinin müfredatına eğitim müdahalelerin dahil edilmesi gerekliliğini göstermektedir. Ayrıca, iyi yapılandırılmış web tabanlı kitlesel çevrimiçi kurslar, aile hekimlerinin sağlıklı yaşam biçimi davranışları ve yaşam biçimi tıbbı uygulamalarına yönelik eğitim gereksinimlerini karşılamada etkili bir çözüm olabilir.
Citation - WoS: 19
Citation - Scopus: 23
Analysis of a Two-Party Supply Chain With Random Disruptions
(Elsevier Science Bv, 2012) Sargut, F. Zeynep; Qi, Lian
We study a continuous-review inventory problem of a two-echelon supply chain with random disruptions, identify properties of the optimal cost function, compare the optimal order quantity with the classical economic order quantity, analyze the sensitivity of the optimal solution, and explore the conditions under which zero-inventory ordering policy is preferred. (C) 2011 Elsevier B.V. All rights reserved.
Analysis of Factors Which Affect the Color Preferences of Consumers in Clothing Sector: Turkish Case
(Ege Universitesi, 2014) Atilgan, Turan; Kanat, Seher; Kuleli, Seda
The meeting rate of consumer desires and expectations is one of the most significant competition factors in clothing sector which is an unstable and dynamic sector. Therefore, the enterprises must give the necessary importance to consumers' desires and expectations. The consumers are affected by many factors such as quality, price and design while making a selection between different clothing brands. Design factor comes into prominence in recent years and color is one of the most significant factors of design. As it is already known, colors affect the buying behavior of consumers. This study aims to analyze the factors which affect the color preferences of consumers in clothing sector. In accordance with the aim of the research, a questionnaire form consisting of 20 questions is prepared. This survey is conducted within March 2014 at the hinterland of Izmir province residents by using face to face interview method. After the conduction of the survey, collected data are evaluated with SPSS 16.0 program. According to the results of our research, Turkish consumers give great importance to the colors and color harmony of garments which they have bought. Black, blue and navy blue are the most preferred garment colors whereas orange, pink and yellow are the least preferred ones. In addition to these, relationships between the demographic properties of consumers and color preferences are analyzed and sectoral suggestions are made.
Citation - WoS: 2
Citation - Scopus: 2
Analysis of Nucleotide Changes in Rt-Pcr Primer/Probe Binding Regions in Sars-Cov Isolates Reported From Turkey
(Ankara Microbiology Soc, 2021) Demir, Ayse Banu; Bulgurcu, Alihan; Appak, Ozgur; Sayiner, Ayca Arzu
The SARS-CoV-2 virus, which caused the COVID-19 epidemic, caused more than 55 million cases and nearly 1.5 million deaths worldwide. For the microbiological diagnosis of the disease, the most valid method is detecting the presence of the viral genome by real-time reverse transcription polymerase chain reaction (rRT-PCR). However, due to the nature of the RNA viruses, frequent mutations may affect the sensitivity of the analyses made on the genetic material of the virus, such as PCR. In this study, we aimed to investigate the mutations in the primer-probe binding regions of the rRT-PCR panels used in COVID-19 diagnosis. SARS-CoV-2 whole genome sequence data (n= 194) isolated from COVID-19 cases in Turkey and uploaded on GISAID database from the centers in Istanbul (n= 78), Ankara (n= 58), Kars (n= 47), Bursa (n= 2), Adiyaman (n= 2), Erciyes (n= 1) and Kocaeli (n= 1) between March 17-September 14, 2020 were analyzed. In order to determine the nucleotide changes, SARS-CoV-2 sequences from Turkey were compared to the reference genome sequence (NC_045512.1) present in GenBank website. The constructed data set was aligned using the MAFFT program and was checked manually if the sequences were in the same frame by using the AliView program. Primer-probe binding sites of the thirteen SARS-CoV-2 rRT-PCR panels from seven different institutes (US CDC, China CDC, Charite CDC, Pasteur, HKU, Thailand, NIID) that are being used in COVID-19 diagnosis were evaluated in terms of nucleotide changes within the corresponding regions compared to the reference genome. Sequence diversities in the viral genomes were determined via positional nucleotide numerical calculator and entropy calculator modules and nucleotide and entropy changes in primer-probe binding regions for each rRT-PCR panel were examined. Among thirteen different primer-probe panels, nucleotide changes in the target regions of the seven primer-probe panels were determined. When viral sequences with nucleotide changes in the primer-probe binding regions were examined, the most common changes were observed in the China CDC N-forward primer and US CDC N3-forward primer binding regions. It is important that the kits to be used as diagnostic tests are designed specific to the regions with less nucleotide changes. Nucleotide changes may not be critical for DNA amplification for most PCR panels, but should be carefully monitored as they may affect the sensitivity of the assay. If the risk of alteration of the designed region is high, the primer - probe binding sites should be checked frequently and updated when necessary.
Citation - Scopus: 2
Analysis of the Factors Affecting Organizational Commitment: an Empirical Application
(Deniz Publication, 2021) Ceylan, C.; Taçoğlu, C.; Kartal, H.
The purpose of this study is to explore the factors affecting organizational commitment, to analyze relations between these factors by introducing a new model, and to reveal how these factors specifically affect work commitment and intention to quit the job. The factors were pooled after extensive literature research. A two-phase pilot study was applied, along with normality tests, factor analysis, discriminant validity, and regression analysis. The final form of the survey was conducted with 205 participants actively employed in an organization. Important findings of the study suggest that work commitment is positively affected by organizational trust, the importance of the job, affective commitment, normative commitment, and negatively affected by self-confidence. Organizational trust and affective commitment negatively affect intention to quit, whereas self-confidence and talent positively affect intention to quit. By introducing an originally proposed organizational commitment model, this study presents an up-to-date analysis of some overlooked factors in the literature and suggests new factors potentially affecting organizational commitment, work commitment, and intention to quit. The outputs of this study can be utilized by organizations in making strategic decisions about indubitably one of the most precious assets of organizations: employees. © 2021, Author. All rights reserved.
Citation - WoS: 10
Citation - Scopus: 11
An Analysis of the Sustainability Disclosures of Textile and Apparel Companies in Turkey
(Ege Univ, 2019) Saygili, Ebru; Saygılı, Arıkan Tarık; Yargi, Seher Goren
The aim of this study is to discuss recent sustainability developments in the textile industry and to explore the disclosures of textile and apparel companies in Turkey. The economic, environmental and social sustainability disclosures of 34 companies were analyzed in relation to Global Reporting Initiative (GRI) standards. The results of the analysis revealed that the most frequently mentioned sustainability issues were related to employees, water, product, market, supplier, education, economic, energy and waste. A majority of companies provided economic disclosures about economic performance and anti-competition; environmental disclosures about water and effluents and energy; and social sustainability disclosures about training, education, employment, health and safety, diversity and equal opportunity, marketing and labeling.

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